Relationship among seminal characteristics, fertility and suitability for semen preservation in draft stallions.
Abstract: Seminal characteristics, fertility and the response to semen preservation (liquid storage and cryopreservation) were evaluated in 4 Draft stallions (Percheron 2, Breton 2). Seminal characteristics (gel-free volume, sperm concentration, sperm morphology, percentage of motile spermatozoa) were assessed in 5 ejaculates from each of the 4 stallions. The fertility of the stallions was calculated retrospectively as the accumulated pregnancy rate over 3 breeding seasons. Five ejaculates from each of the stallions were subjected to liquid storage at 5 degrees C. The percentage of motile spermatozoa (PMS) was assessed at 0, 24, 48 and 72 hr. Cryopreservation was done with a glucose-EDTA-lactose egg yolk extender and a skim milk-based extender containing clarified egg yolk (4 replicates per stallion). PMS and the percentage of spermatozoa with an intact plasma membrane (IPM) were evaluated before and after freezing. Although semen characteristics and fertility were within a physiological range, significant differences among stallions were found for gel-free volume, percentage of morphologically normal spermatozoa, PMS, and fertility but there was no clear relationship between these parameters. The response to liquid storage of diluted semen samples differed among stallions and best results were obtained in a stallion with the highest initial motility. The freezability of semen from the 4 stallions was poor with both of the freezing protocols (PMS range 13.8%-26.3%; IPM range 19.5%-38.0%). Results of liquid storage and cryopreservation were not correlated in individual stallions and were not related to their fertility. Since predictability for the response to semen preservation is low, the suitability of individual stallions for semen preservation has to be examined carefully.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1995-04-01 PubMed ID: 7492637DOI: 10.1292/jvms.57.225Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research paper investigates the relationship between seminal characteristics, fertility, and the suitability for semen preservation in 4 Draft stallions. Despite differences between the stallions in seminal volume, sperm morphology and motility, and fertility, no clear link was found between these factors. The study shows that the ability to preserve semen differs from stallion to stallion and is not related to fertility. It concludes that the suitability of individual stallions for semen preservation must be carefully assessed.
Sampling and Assessment
- The researchers evaluated the seminal characteristics, fertility, and response to semen preservation in 4 Draft stallions of Percheron and Breton breeds.
- Characteristics of semen such as gel-free volume, sperm concentration, sperm morphology, and the percentage of motile sperm were assessed in 5 ejaculates from each of the 4 stallions.
- Stallion fertility was retrospectively calculated based on the accumulated pregnancy rate over three breeding seasons.
Semen Preservation Study
- Five ejaculates from each stallion were subjected to liquid storage at 5 degrees Celsius.
- The percentage of motile sperm was assessed at 0, 24, 48, and 72 hours.
- Cryopreservation was performed using a glucose-EDTA-lactose egg yolk extender and a skim milk-based extender containing clarified egg yolk, with four replicates per stallion.
- The percentage of sperm with intact plasma membranes (IPM) and motile sperm were evaluated before and after freezing.
Findings and Conclusion
- Semen characteristics and fertility varied among the four stallions, but there was no evident relationship between these parameters.
- The response to liquid storage differed among stallions, and the best results were obtained from a stallion with the highest initial motility.
- The researchers found poor results for semen freezability using both freezing protocols (PMS range 13.8%-26.3%; IPM range 19.5%-38.0%).
- Results of liquid storage and cryopreservation did not correlate in individual stallions and were not related to their fertility.
- The study concluded that the response to semen preservation is difficult to predict, thus a thorough examination is needed to determine individual stallions’ suitability for semen preservation.
Cite This Article
APA
Torres-Boggino F, Sato K, Oka A, Kanno Y, Hochi S, Oguri N, Braun J.
(1995).
Relationship among seminal characteristics, fertility and suitability for semen preservation in draft stallions.
J Vet Med Sci, 57(2), 225-229.
https://doi.org/10.1292/jvms.57.225 Publication
Researcher Affiliations
- Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
MeSH Terms
- Animals
- Cryopreservation
- Ejaculation
- Fertility
- Horses
- Male
- Semen / physiology
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Count
- Sperm Motility
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
