Relationship between arthroscopic joint evaluation and the levels of Coll2-1, Coll2-1NO(2), and myeloperoxidase in the blood and synovial fluid of horses affected with osteochondrosis of the tarsocrural joint.
Abstract: To evaluate the levels of plasmatic and synovial Coll2-1, Coll2-1NO(2) and myeloperoxidase (MPO) in horses with osteochondral lesions of the tarsocrural joint and to investigate how these levels relate to arthroscopic findings of inflammation and degeneration. Methods: Venous blood and synovial fluid samples were collected from 63 horses presented for arthroscopic removal of osteochondral fragments in the tarsocrural joint. Prior to removal of the osteochondral fragment, an exploration of the joint was performed and an inflammatory and degenerative score was determined. The blood and synovial levels of Coll2-1, Coll2-1NO(2) and MPO were also measured. The effects of the arthroscopic evaluation (inflammatory and degenerative classes) on the blood and synovial markers were evaluated using a linear model (GLM procedure), and correlations between biochemical markers in the blood and synovial fluid and the arthroscopic evaluation (inflammatory and degenerative classes) were established (Pearson's correlations). Results: Significantly higher levels of Coll2-1 were detected in synovial fluid of higher degenerative classes. There was a significant correlation between the degenerative score and the synovial levels of Coll2-1 (r=0.27). According to the logistic regression model, there was a significant effect of the degenerative class on synovial levels of Coll2-1. Conclusions: Coll2-1 correlates well with the degenerative state of tarsocrural joints as evaluated by arthroscopy. This marker can therefore be classified as a burden-of-disease marker in the assessment of joint disease in horses.
Copyright © 2011 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Publication Date: 2011-08-16 PubMed ID: 21884810DOI: 10.1016/j.joca.2011.08.002Google Scholar: Lookup
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Summary
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The research paper investigates the relationship between the presence of certain substances in the blood and synovial fluid of horses and arthroscopic findings of inflammation and degeneration in their tarsocrural joints, which are affected by osteochondrosis.
Research Methodology
- The study involves the collection of venous blood and synovial fluid samples from 63 horses proposed for arthroscopic removal of osteochondral fragments in the tarsocrural joint.
- Arthroscopic exploration of the joint was carried out before the removal of osteochondral fragments to ascertain an inflammation and degeneration score.
- Subsequently, Coll2-1, Coll2-1NO(2), and myeloperoxidase (MPO) levels in blood and synovial fluid samples of horses were evaluated.
- The impact of the inflammatory and degenerative classes observed during arthroscopic examination on these biochemical markers were studied using a statistical tool called General Linear Model (GLM).
- Lastly, by utilizing Pearson’s correlation factor, correlations between the noted biochemical markers and the findings from the arthroscopic evaluation (both inflammatory and degenerative) were established.
Research Findings
- Noteworthy higher levels of Coll2-1 were identified in the synovial fluid of horses that had higher degenerative rates, indicating a connection between this biochemical marker and joint degeneration.
- Statistically significant correlation (r=0.27) was observed between the degenerative score determined through arthroscopy and synovial fluid levels of Coll2-1.
- According to the logistic regression model, a statistically significant impact of the degenerative class on the synovial levels of Coll2-1 was reported.
Research Conclusion
- The primary conclusion of the study is that the degenerative state of the tarsocrural joints in horses, as evaluated by arthroscopy, correlates well with the presence of Coll2-1 in the synovial fluid.
- This reveals the potential of employing this marker, Coll2-1, as a “burden-of-disease” indicator for assessing the severity of joint diseases in horses.
Cite This Article
APA
Verwilghen DR, Enzerink E, Martens A, Franck T, Balligand M, Henrotin Y, Detilleux J, Serteyn D.
(2011).
Relationship between arthroscopic joint evaluation and the levels of Coll2-1, Coll2-1NO(2), and myeloperoxidase in the blood and synovial fluid of horses affected with osteochondrosis of the tarsocrural joint.
Osteoarthritis Cartilage, 19(11), 1323-1329.
https://doi.org/10.1016/j.joca.2011.08.002 Publication
Researcher Affiliations
- Department of Companion Animals and Equids, Faculty of Veterinary Medicine of Liege, Sart-Tilman B41, B-4000 Liege, Belgium. denis@proamhorses.eu
MeSH Terms
- Animals
- Arthroscopy
- Biomarkers / metabolism
- Collagen Type II / metabolism
- Hindlimb / metabolism
- Horse Diseases / classification
- Horse Diseases / diagnosis
- Horse Diseases / metabolism
- Horses
- Joint Diseases / classification
- Joint Diseases / diagnosis
- Joint Diseases / metabolism
- Joint Diseases / veterinary
- Osteochondrosis / classification
- Osteochondrosis / diagnosis
- Osteochondrosis / metabolism
- Osteochondrosis / veterinary
- Peptide Fragments / metabolism
- Peroxidase / metabolism
- Synovial Fluid / chemistry
- Tarsal Joints / metabolism
- Tarsal Joints / pathology
Citations
This article has been cited 3 times.- Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in Horses - Molecular Background of its Pathogenesis and Perspectives for Progenitor Stem Cell Therapy.. Stem Cell Rev Rep 2019 Jun;15(3):374-390.
- Legrand CB, Lambert CJ, Comblain FV, Sanchez C, Henrotin YE. Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models.. Cartilage 2017 Jul;8(3):211-233.
- Niemelä T, Virén T, Liukkonen J, Argüelles D, te Moller NC, Puhakka PH, Jurvelin JS, Tulamo RM, Töyräs J. Application of optical coherence tomography enhances reproducibility of arthroscopic evaluation of equine joints.. Acta Vet Scand 2014 Jan 10;56(1):3.
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