Relationship between hepatic grayish-white solid nodules in horses imported from Canada and larval Echinococcus multilocularis infection.
Abstract: Histopathological and genetic examinations were conducted on grayish-white solid hepatic nodules in 150 horses imported from Canada, in order to investigate larval Echinococcus multilocularis infection. Ten of the 150 horses (6.7%) were diagnosed with alveolar hydatid disease. The sequences of the mitochondrial cytochrome b genes obtained from all 10 polymerase chain reaction positive samples had 99 to 100% identity with the European haplotype E1 of E. multilocularis. Therefore, we concluded that the infections likely originated in Canada. Relation entre les nodules hépatiques solides blanc-grisâtre trouvés chez des chevaux importés du Canada et l’infection larvaire à Echinococcus multilocularis . Des examens histopathologiques et génétiques ont été effectués sur des nodules hépatiques solides blanc-grisâtre observés chez 150 chevaux importés du Canada afin d’étudier l’infection larvaire à Echinococcus multilocularis. Dix des 150 chevaux (6,7 %) ont reçu un diagnostic de maladie hydatique alvéolaire. Les séquences des gènes mitochondriaux du cytochrome b obtenus à partir des 10 échantillons positifs par réaction d’amplification en chaîne par la polymérase ont montré une identité de 99 à 100 % avec l’haplotype européen E1 d’E. multilocularis. L’haplotype d’E. multilocularis obtenu à partir de cette étude suggère que les infections sont probablement originaires du Canada.(Traduit par Dr Serge Messier).
Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.
Publication Date: 2021-03-12 PubMed ID: 33692585PubMed Central: PMC7877675
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- Journal Article
Summary
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This research examines the relationship between the occurrence of grayish-white solid liver nodules in horses imported from Canada and their infection by Echinococcus multilocularis larvae. The study establishes that 6.7% of the sample horses were diagnosed with alveolar hydatid disease, an infection commonly caused by the mentioned parasite.
Methodology and Findings
- The researchers carried out histopathological and genetic examinations on liver nodules found in 150 horses imported from Canada.
- The objective behind the examination was to better understand the correlation between these liver manifestations and a parasitic infection primarily caused by the larvae of Echinococuus multilocularis.
- Out of the total sample of 150 horses, 10 of them (equating to 6.7%) were conclusively diagnosed with what is referred to as ‘alveolar hydatid disease’. This disease is essentially a parasitic infection characterized by the presence of alveolar echinococcosis in the liver.
Genetic Analysis
- A genetic analysis was conducted on the biopsied samples of the horses that tested positive for the disease. These were analyzed for sequences of mitochondrial cytochrome b genes.
- The mitochondrial cytochrome b gene sequences found in the positive samples showed 99 to 100% match to the European haplotype E1 of Echinococcus multilocularis.
- This is a significant finding since it suggests a strong possibility of the infection originating in Canada, meaning the horses contracted the disease prior to being imported.
Conclusion
- Based on the genetic match to the European haplotype E1 of Echinococcus multilocularis and the presence of these liver nodules in such a significant number of horses, the study concludes that the infection most likely originated in Canada.
- This indicates a need for further investigation into the prevalence and control of Echinococuus multilocularis infection in Canadian horses, as well as in imported horse livestock in general.
Cite This Article
APA
Hifumi T, Tanaka T, Hernandez EP, Akioka K, Yamada K, Imamura Y, Hatai H, Miyoshi N.
(2021).
Relationship between hepatic grayish-white solid nodules in horses imported from Canada and larval Echinococcus multilocularis infection.
Can Vet J, 62(3), 285-288.
Publication
Researcher Affiliations
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
- Transboundary Animal Diseases Research Center (Hifumi), Laboratory of Veterinary Histopathology (Hifumi, Hatai, Miyoshi), Laboratory of Infectious Diseases (Tanaka, Hernandez), Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Kumamoto Prefectural Meat Inspection Office, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto, 861-1344, Japan (Akioka, Yamada, Imamura).
MeSH Terms
- Animals
- Canada
- Echinococcosis / veterinary
- Echinococcosis, Hepatic / veterinary
- Echinococcus multilocularis / genetics
- Horse Diseases
- Horses
- Larva
References
This article includes 15 references
- Deplazes P, Eckert J. Veterinary aspects of alveolar echinococcosis — A zoonosis of public health significance.. Vet Parasitol 2001;98:65–87.
- Moro P, Schantz PM. Echinococcosis: A review.. Int J Infect Dis 2009;13:125–133.
- Massolo A, Liccioli S, Budke C, Klein C. Echinococcus multilocularis in North America: The great unknown.. Parasite 2014;21:73.
- Peregrine AS, Jenkins EJ, Barnes B. Alveolar hydatid disease (Echinococcus multilocularis) in the liver of a Canadian dog in British Columbia, a newly endemic region.. Can Vet J 2012;53:870–874.
- Gesy K, Hill JE, Schwantje H, Liccioli S, Jenkins EJ. Establishment of a European-type strain of Echinococcus multilocularis in Canadian wildlife.. Parasitology 2013;140:1133–1137.
- Catalano S, Lejeune M, Liccioli S. Echinococcus multilocularis in urban coyotes, Alberta, Canada.. Emerg Infect Dis 2012;18:1625–1628.
- Goto Y, Sato K, Yahagi K. Frequent isolation of Echinococcus multilocularis from the livers of racehorses slaughtered in Yamagata, Japan.. Jpn J Infect Dis 2010;63:449–451.
- Shiryou Uma Kankei. Ministry of Agriculture, Forestry and Fisheries 2017. 2016. [Last accessed January 21, 2021]. (in Japanese) [homepage on the Internet] [updated 2017 March]. Available from: https://www.maff.go.jp/j/chikusan/kikaku/lin/attach/pdf/sonota-25.pdf.
- Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T. Detection of Echinococcus multilocularis in the definitive host: Coprodiagnosis by PCR as an alternative to necropsy.. J Clin Microbiol 1998;36:1871–1876.
- Gesy KM, Jenkins EJ. Introduced and native haplotypes of Echinococcus multilocularis in wildlife in Saskatchewan, Canada.. J Wildl Dis 2015;51:743–748.
- Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing.. Mol Biochem Parasitol 1992;54:165–173.
- Simsek S, Roinioti E, Eroksuz H. First report of Echinococcus equinus in a donkey in Turkey.. Korean J Parasitol 2015;53:731–735.
- Echinococcosis in Japan, 1999–2018 [homepage on the Internet] [Last accessed January 21, 2021];Infectious Agents Surveillance Report. 40:33–34. [updated 2019 March]. Available from: https://www.niid.go.jp/niid/en/iasr-vol33-e/865-iasr/8695-469te.html.
- Nakao M, Xiao N, Okamoto M, Yanagida T, Sako Y, Ito A. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis.. Parasitol Int 2009;58:384–389.
- Gesy KM, Schurer JM, Massolo A. Unexpected diversity of the cestode Echinococcus multilocularis in wildlife in Canada.. Int J Parasitol Parasites Wildl 2014;3:81–87.
Citations
This article has been cited 4 times.- Hifumi T, Tanaka T, Suzu I, Sato M, Akioka K, Fujimata C, Shinkai R, Maeda T, Kusakisako K, Ikadai H, Miyoshi N. Molecular phylogenetic analysis of Echinococcus multilocularis from horses raised in Canada or Japan, using mitochondrial cytochrome b gene-targeted PCR. Food Waterborne Parasitol 2024 Mar;34:e00219.
- Delling C, Helm C, Heinze P, Friedman M, Böttcher D. First report of infection with metacestode stages of Echinococcus multilocularis in a kulan (Equus hemionus kulan) from Slovakia. Int J Parasitol Parasites Wildl 2023 Dec;22:80-83.
- Hifumi T, Tanaka T, Sato M, Akioka K, Fujimata C, Miyoshi N. Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay. Vet Anim Sci 2023 Jun;20:100291.
- Robbins WT, Galeuzzi O, Graham K, Greenwood SJ, Jones MEB, Buote M, Conboy GA. Echinococcus multilocularis infection in a red fox (Vulpes vulpes) on Prince Edward Island, Canada. Can Vet J 2022 Sep;63(9):962-966.
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