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Journal of animal physiology and animal nutrition2017; 102(1); e380-e386; doi: 10.1111/jpn.12756

Renal energy excretion of horses depends on renal hippuric acid and nitrogen excretion.

Abstract: The prediction of renal energy excretion is crucial in a metabolizable energy system for horses. Phenolic acids from forage cell walls may affect renal energy losses by increasing hippuric acid excretion. Therefore, the relationships were investigated between renal energy, nitrogen (N) and hippuric acid excretion of four adult ponies (230-384 kg body weight (BW)) consuming diets based on fresh grass, grass silage, grass cobs (heat-dried, finely chopped, pressed grass), alfalfa hay, straw, extruded straw and soybean meal. Feed intake was measured; urine and faeces were quantitatively collected for three days. Feed was analysed for crude nutrients, gross energy, amino acids and neutral-detergent-insoluble crude protein (CP); faeces were analysed for crude nutrients and cross energy; urine was analysed for N, hippuric acid, creatinine and gross energy. Renal energy excretion (y; kJ/kg BW ) correlated with renal N excretion (x ; g/kg BW ) and renal hippuric acid excretion (x ; g/kg BW ): y = 14.4 + 30.2x +20.7x (r = .95; n = 30; p < .05). Renal hippuric acid excretion was highest after intake of fresh grass and lowest after intake of soybean meal. The ratio of hippuric acid to creatinine in urine and the excretion of hippuric acid per gram of dry matter intake was significantly higher for fresh grass than for all other rations. There was no relationship between aromatic amino acid intake and renal hippuric acid excretion. The results of the present study and literature data suggest that feed can be categorized into four groups with regard to the energy losses per gram CP intake: (i) protein supplements (e.g., soybean meal): 4.2-4.9 kJ/g CP intake (ii) alfalfa hay, grains, dried sugar beet pulp: 6.4 kJ/g CP intake, (iii) hay, preserved grass products, straw: 5.2-12.3 kJ/g CP intake (mean 8) and (iv) fresh grass. For group (iii) a negative relationship was observed between renal energy losses per gram of CP and the content of CP or neutral-detergent-insoluble CP in dry matter.
Publication Date: 2017-06-13 PubMed ID: 28608590DOI: 10.1111/jpn.12756Google Scholar: Lookup
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Summary

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The study explores how the dietary intake of horses, particularly differing feeds, impacts renal energy, nitrogen, and hippuric acid excretion. It finds the highest hippuric acid excretion with grass intake and the lowest with soybean meal consumption.

Study Overview

  • The study focuses on predicting renal energy excretion, critical in determining the metabolizable energy system for horses.
  • The source of the feed, namely fresh grass, grass silage, grass cobs, alfalfa hay, straw, extruded straw and soybean meal, was manipulated to evaluate their effects on renal energy, nitrogen, and hippuric acid excretion.
  • Using four adult ponies of varying body weight, the researchers administered these various diets and subsequently analysed the feed, urine, and faeces for three days to measure different parameters.

Key Results

  • The researchers observed a correlation between renal energy excretion and renal nitrogen excretion, and hippuric acid excretion. Renal hippuric acid excretion was highest after the intake of fresh grass and lowest after the intake of soybean meal.
  • The excretion of hippuric acid per gram of dry matter intake was significantly higher after consuming fresh grass compared to other rations. However, there was no detected relationship between aromatic amino acid intake and renal hippuric acid excretion.

Feed Categorisation

  • Based on the study results and existing literature data, feeds were proposed to be categorised into four groups. Each group represented differentiated energy losses per gram crude protein (CP) intake. For instance, protein supplements, including soybean meal, demonstrated 4.2-4.9 kJ/g CP intake energy loss.
  • Notably, for the group including hay, preserved grass products, and straw, the researchers spotted a negative relationship between renal energy losses per gram of CP and the content of CP, or neutral-detergent-insoluble CP in dry matter.

Implications

  • The research findings offer insights into optimising horse nutrition by understanding how various feed types impact renal energy excretion.
  • It also establishes an understanding of how certain feed influences the urinary excretion of hippuric acid, which is a crucial parameter in nutritional adjustment for horses.

Cite This Article

APA
Hipp B, Südekum KH, Zeyner A, Goren G, Kienzle E. (2017). Renal energy excretion of horses depends on renal hippuric acid and nitrogen excretion. J Anim Physiol Anim Nutr (Berl), 102(1), e380-e386. https://doi.org/10.1111/jpn.12756

Publication

ISSN: 1439-0396
NlmUniqueID: 101126979
Country: Germany
Language: English
Volume: 102
Issue: 1
Pages: e380-e386

Researcher Affiliations

Hipp, B
  • Chair of Animal Nutrition and Dietetics, Department of Veterinary Science, Ludwig-Maximilians-University, Munich, Germany.
Südekum, K-H
  • Institute of Animal Science, University of Bonn, Bonn, Germany.
Zeyner, A
  • Institute of Agricultural and Nutritional Sciences, Animal Nutrition Group, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Goren, G
  • Chair of Animal Nutrition and Dietetics, Department of Veterinary Science, Ludwig-Maximilians-University, Munich, Germany.
Kienzle, E
  • Chair of Animal Nutrition and Dietetics, Department of Veterinary Science, Ludwig-Maximilians-University, Munich, Germany.

MeSH Terms

  • Animal Feed / analysis
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Cross-Over Studies
  • Diet
  • Dietary Proteins
  • Dietary Supplements
  • Digestion
  • Female
  • Hippurates / metabolism
  • Horses / physiology
  • Kidney / metabolism
  • Male
  • Medicago sativa
  • Nitrogen / chemistry
  • Nitrogen / metabolism
  • Poaceae
  • Soybeans

Citations

This article has been cited 3 times.
  1. Saastamoinen M, Särkijärvi S, Suomala H. Protein Source and Intake Effects on Diet Digestibility and N Excretion in Horses-A Risk of Environmental N Load of Horses. Animals (Basel) 2021 Dec 15;11(12).
    doi: 10.3390/ani11123568pubmed: 34944342google scholar: lookup
  2. Ebert M, Moore-Colyer MJS. The energy requirements of racehorses in training. Transl Anim Sci 2020 Oct;4(4):txaa196.
    doi: 10.1093/tas/txaa196pubmed: 33367221google scholar: lookup
  3. Ebert M, Moore-Colyer MJS. The energy requirements of performance horses in training. Transl Anim Sci 2020 Apr;4(2):txaa032.
    doi: 10.1093/tas/txaa032pubmed: 32705030google scholar: lookup