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Home / Equine Research Bank / Ann Biomed Eng / Repair of extensive articular cartilage defects in horses by...
Annals of biomedical engineering2004; 32(1); 57-69; doi: 10.1023/b:abme.0000007791.81433.1a

Repair of extensive articular cartilage defects in horses by autologous chondrocyte transplantation.

Abstract: Damaged adult articular cartilage has very limited capacity to heal. Autologous chondrocyte transplantation (ACT) has been used clinically and studied in experimental animals in an attempt to provide biologically based cartilage regeneration. This study evaluated cartilage repair following ACT in a large animal model over a period of 2 years. Articular cartilage defects (10 mm in diameter, full-thickness) were created in the minor load-bearing area on the lateral talus of tibiotarsal joints of eight adult horses. In each animal, the right joint was repaired using autologous chondrocytes injected beneath the periosteum, as in the original ACT procedure (Brittberg, M., A. Lindahl, A. Nilsson, C. Ohlsson, O. Isaksson, and L. Peterson N. Engl. J. Med. 331:889-895, 1994): the left joint remained untreated to serve as a control. Clinical and pathological evaluation was within the range of normal for all horses at both time points. Compared to untreated defects, ACT resulted in significantly improved defect filling with a well-integrated neocartilage and comparable expression of cartilage-specific markers. The histological score (Peterson, L., T. Minas, M. Brittberg, A. Nilsson, E. Sjogren-Jansson, and A. Lindahl Clin. Orthop. 374:212-234, 2000) (10.4 +/- 0.9 for ACT and 5.6 +/- 3.9 for controls, all animals, p = 0.016) indicated that ACT contributed to the reparative process. For the first time, the efficacy of ACT was demonstrated in a large animal model supporting the potential of ACT for cartilage regeneration in patients.
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Publication Date: 2004-02-18 PubMed ID: 14964722DOI: 10.1023/b:abme.0000007791.81433.1aGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper examines the effects of using autologous chondrocyte transplantation to repair a significant amount of damage to articular cartilage in horses, finding that it improves the healing process.

Introduction to the Study

  • The paper is a research study that investigates the effectiveness of autologous chondrocyte transplantation (ACT) in repairing extensive cartilage damage in horses.
  • Articular cartilage with extensive damage is capable of very limited healing in adulthood, hence experimental and clinical applications of ACT are being conducted to facilitate bio-based regeneration of cartilage.
  • The prolonged study duration of 2 years allows for comprehensive evaluation of postoperative recovery and the long-term effects of ACT.

Methodology

  • Researchers created articular cartilage defects deliberately, each 10mm in diameter and full-thickness, in the lateral talus region of the tibiotarsal joints in eight horses, an area that bears minor load in the body structure.
  • In each horse, the right joint was treated using ACT in which autologous chondrocytes were injected into the area beneath the periosteum; this is the original ACT procedure.
  • The left joint in each horse, with the same level of defect, was not treated and served as a control for comparing the effectiveness of ACT.

Findings

  • After the period of two years, all the horses were clinically and pathologically evaluated and found to be within the normal limits.
  • Compared to the untreated control joints, the ACT-treated joints showed better defect filling, with the formation of a well-integrated neocartilage that exhibited equivalent expression of cartilage-specific markers, indicating successful ACT.
  • The histological scoring system, a method to quantify the effectiveness of the ACT, showed a better score for the ACT-treated joints compared to the untreated controls (10.4 +/- 0.9 vs 5.6 +/- 3.9). This statistically significant difference suggests that ACT positively impacted the regenerative process of cartilage.

Conclusion

  • This study is crucial as it demonstrates the successful application and effectiveness of autologous chondrocyte transplantation in a large animal model for the first time.
  • The findings hold promise for the potential use of ACT in human patients for the purpose of cartilage regeneration.

Cite This Article

APA
Litzke LE, Wagner E, Baumgaertner W, Hetzel U, Josimović-Alasević O, Libera J. (2004). Repair of extensive articular cartilage defects in horses by autologous chondrocyte transplantation. Ann Biomed Eng, 32(1), 57-69. https://doi.org/10.1023/b:abme.0000007791.81433.1a

Publication

Annals of biomedical engineering
ISSN: 0090-6964
NlmUniqueID: 0361512
Country: United States
Language: English
Volume: 32
Issue: 1
Pages: 57-69

Researcher Affiliations

Litzke, L E
  • co.don AG, Warthestrasse 21, Teltow, Germany.
Wagner, E
    Baumgaertner, W
      Hetzel, U
        Josimović-Alasević, O
          Libera, J

            MeSH Terms

            • Animals
            • Cartilage Diseases / surgery
            • Cartilage, Articular / injuries
            • Cartilage, Articular / pathology
            • Cartilage, Articular / surgery
            • Cell Transplantation / methods
            • Chondrocytes / pathology
            • Chondrocytes / transplantation
            • Collagen / metabolism
            • Culture Techniques / methods
            • Disease Models, Animal
            • Hindlimb / injuries
            • Hindlimb / pathology
            • Hindlimb / surgery
            • Horses
            • Transplantation, Autologous / methods
            • Treatment Outcome
            • Wound Healing / physiology

            Citations

            This article has been cited 12 times.
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