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Journal of veterinary internal medicine2017; 31(4); 1178-1187; doi: 10.1111/jvim.14725

Repeatability of Oral Sugar Test Results, Glucagon-Like Peptide-1 Measurements, and Serum High-Molecular-Weight Adiponectin Concentrations in Horses.

Abstract: Repeatability of the oral sugar test (OST) has not been evaluated. Objective: We hypothesized that OST glucose, insulin, active (aGLP-1) and total (tGLP-1) glucagon-like peptide 1, and high-molecular-weight (HMW) adiponectin results would be repeatable. Methods: Fifty-three horses from a Tennessee research facility (n = 23) and private practice in Missouri (n = 30), including animals with medical histories of equine metabolic syndrome. Methods: Two OSTs were performed 7-14 days apart and plasma glucose and insulin concentrations were measured at 0, 60, and 75 minutes; a positive result was defined as detection of an insulin concentration >45 μU/mL at 60 or 75 minutes. Plasma aGLP-1 and serum tGLP-1 concentrations at 75 minutes and serum HMW adiponectin concentrations at 0 minute were measured in the Missouri group. Bland-Altman analyses were performed. Results: No adverse events were reported. Bland-Altman analysis indicated mean ± SD bias of 1.5 ± 14.8 μU/mL (95% confidence interval [CI], -27.6 to 30.5 μU/mL) and 1.2 ± 16.1 μU/mL (95% CI, -30.4 to 32.8 μU/mL) for insulin concentrations at 60 and 75 minutes, respectively. There was 91 and 83% agreement in test interpretation between test days for OST insulin results for all horses in the Tennessee and Missouri groups, respectively. Conclusions: Repeatability of the OST was acceptable when values obtained from Bland-Altman analyses were evaluated, and there was good agreement in binary (negative/positive) test interpretation for insulin concentrations. However, wide 95% CIs were detected for insulin concentrations.
Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2017-05-24 PubMed ID: 28543933PubMed Central: PMC5508339DOI: 10.1111/jvim.14725Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study assessed the repeatability of the oral sugar test (OST) results in horses, focusing on measurements of glucose, insulin, glucagon-like peptide 1 (both active and total amounts), and high-molecular-weight adiponectin. The study found that OST results were consistent and the agreement in test interpretation between tests for insulin concentrations was high, although a wide 95% confidence interval was observed for insulin concentrations.

Objective and Hypothesis

  • The research aimed to determine the repeat reliability of the oral sugar test (OST), a tool used for monitoring glucose and insulin concentrations in horses. The researchers hypothesized that the results from tests on glucose, insulin, active and total glucagon-like peptide 1, and high-molecular-weight adiponectin would be consistently repeatable.

Methodology

  • The study involved 53 horses from a research facility in Tennessee and a private practice in Missouri.
  • An OST was performed twice on each horse within a 7-14 day interval. Blood samples were extracted at three specific intervals (0, 60, and 75 minutes), and the glucose and insulin concentrations were measured. A positive result was defined as an insulin concentration higher than 45 µU/mL at either 60 or 75 minutes.
  • For the Missouri group, the concentrations of active and total glucagon-like peptide-1 at 75 minutes and the concentration of high-molecular-weight adiponectin at 0 minute were also measured.
  • Data analysis involved the use of the Bland-Altman analysis method.

Results

  • No adverse events were noted during the study.
  • The Bland-Altman analysis showed similar results for the insulin concentrations at the 60 and 75-minute marks (with a mean bias observed).
  • There was a high rate of agreement in interpreting the test results between the two test days in both the Tennessee (91%) and Missouri (83%) groups, with regards to OST insulin results.

Conclusions

  • The study established acceptable repeatability of the OST when Bland-Altman analyses were considered. This indicates the potential reliability of the OST for continuous testing of horses.
  • There was good consistency observed in the interpretation of negative or positive test results for insulin levels, suggesting this could be a robust binary (negative/positive) test.
  • However, a variable wide 95% confidence interval was noted for insulin concentrations, which implies potential variance in results and calls for continued study and refining of the test.

Cite This Article

APA
Frank N, Walsh DM. (2017). Repeatability of Oral Sugar Test Results, Glucagon-Like Peptide-1 Measurements, and Serum High-Molecular-Weight Adiponectin Concentrations in Horses. J Vet Intern Med, 31(4), 1178-1187. https://doi.org/10.1111/jvim.14725

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 31
Issue: 4
Pages: 1178-1187

Researcher Affiliations

Frank, N
  • Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
Walsh, D M
  • Homestead Veterinary Hospital, Pacific, MO.

MeSH Terms

  • Adiponectin / blood
  • Animals
  • Blood Glucose / analysis
  • Female
  • Glucagon-Like Peptide 1 / blood
  • Glucose Tolerance Test / statistics & numerical data
  • Glucose Tolerance Test / veterinary
  • Horses / blood
  • Insulin / blood
  • Male
  • Reproducibility of Results

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Citations

This article has been cited 12 times.
  1. Stefanovski D, Robinson MA, Van Eps A. Effect of a GLP-1 mimetic on the insulin response to oral sugar testing in horses. BMC Vet Res 2022 Jul 29;18(1):294.
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