Reproductive stage and sex steroid hormone levels influence the expression of mesenchymal stromal cell (MSC) markers in the equine endometrium.
Abstract: Mesenchymal stem or stromal cells (MSCs) play key roles in tissue homeostasis. In the cyclic equine endometrium, this may be regulated by changes in serum concentrations of sex steroid hormones. This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 h and 7days post-partum; n = 5 per stage) and were analyzed by RT-qPCR. MSC (CD29, CD44, CD73, CD90, CD105) and perivascular (CD146, NG2) markers were present in all samples irrespective of reproductive stage. Transcript levels of most markers were present at lowest levels on day 5 after ovulation and at 20 h post-partum. MSCs isolated from endometrial tissue (n = 6 mares) were cultured in the presence of progesterone (0.01-100 μM) and estradiol 17β (0.1-1 μM), and cell proliferation was analyzed using alamarBlue assay. Relative to cells incubated in steroid-depleted media, both progesterone and estradiol 17β moderately increased cell proliferation (1.1- and 1.2-fold, respectively) independently of the concentration used. In conclusion, our results suggest that levels of MSC markers in equine endometrium dynamically change across reproductive cycles and that MSC populations are in part regulated by sex steroids.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication Date: 2018-05-04 PubMed ID: 29775846DOI: 10.1016/j.theriogenology.2018.04.034Google Scholar: Lookup
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Summary
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This research explores the influence of reproductive stage and sex steroid hormone levels on the expression of markers found in mesenchymal stromal cells in the equine endometrium, aiming to better understand the role of these cells in tissue health.
Objective of the Study
- The focus of this study was to examine the changes in endometrial expression of mesenchymal stem or stromal cells (MSCs) markers during the reproductive cycles in mares. This includes the role of sex steroid hormones on endometrial MSC proliferation.
Methods Used to Conduct the Study
- Researchers collected endometrial biopsies from pony mares at different reproductive stages.
- These samples were then analysed by RT-qPCR, a molecular biology technique for detecting RNA expression.
- Mesenchymal stem cells (MSC) that were isolated from endometrial tissue were cultured in the presence of two hormones – progesterone and estradiol 17β.
- Cell proliferation in these cultures was analysed using alamarBlue assay – a method used to measure cell viability, cytotoxicity and proliferation.
Observations from the Study
- MSC and perivascular markers were present in all samples, independent of reproductive stage. The transcript levels of most of these markers were at their lowest on day 5 after ovulation and 20 hours post-partum.
- Both progesterone and estradiol 17β were found to moderately increase cell proliferation.
Conclusion of the Study
- The study concludes that the levels of MSC markers in equine endometrium are subject to change during the course of reproductive cycles.
- The study also reveals that MSC populations are partly regulated by sex steroids.
Cite This Article
APA
Rink BE, Kuhl J, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
(2018).
Reproductive stage and sex steroid hormone levels influence the expression of mesenchymal stromal cell (MSC) markers in the equine endometrium.
Theriogenology, 116, 34-40.
https://doi.org/10.1016/j.theriogenology.2018.04.034 Publication
Researcher Affiliations
- Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies; Artificial Inseminaton and Embryo Transfer, Department for Companion Animals and Horses, Vetmeduni Vienna, Vienna, Austria; The Roslin Institute, University of Edinburgh, Edinburgh, UK.
- Artificial Inseminaton and Embryo Transfer, Department for Companion Animals and Horses, Vetmeduni Vienna, Vienna, Austria.
- The Roslin Institute, University of Edinburgh, Edinburgh, UK.
- Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies.
- Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies.
- Artificial Inseminaton and Embryo Transfer, Department for Companion Animals and Horses, Vetmeduni Vienna, Vienna, Austria. Electronic address: Christine.Aurich@vetmeduni.ac.at.
- The Roslin Institute, University of Edinburgh, Edinburgh, UK.
MeSH Terms
- Animals
- Biomarkers / metabolism
- Cell Proliferation / drug effects
- Endometrium / cytology
- Endometrium / drug effects
- Endometrium / metabolism
- Estradiol / pharmacology
- Female
- Gonadal Steroid Hormones / metabolism
- Horses / metabolism
- Horses / physiology
- Mesenchymal Stem Cells / drug effects
- Mesenchymal Stem Cells / metabolism
- Progesterone / pharmacology
- RNA, Messenger / metabolism
- Sexual Maturation
Citations
This article has been cited 2 times.- Jang HO, Ahn TY, Ju JM, Bae SK, Kim HR, Kim DS. Odontogenic Differentiation-Induced Tooth Regeneration by Psoralea corylifolia L. Curr Issues Mol Biol 2022 May 19;44(5):2300-2308.
- Tian C, He J, An Y, Yang Z, Yan D, Pan H, Lv G, Li Y, Wang Y, Yang Y, Zhu G, He Z, Zhu X, Pan X. Bone marrow mesenchymal stem cells derived from juvenile macaques reversed ovarian ageing in elderly macaques. Stem Cell Res Ther 2021 Aug 18;12(1):460.
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