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Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease.
Abstract: The response of parenchymal strips from guinea-pig lungs to tracheobronchial lavage fluid (TBLF) collected from 8 normal horses and from 8 affected with summer pasture-associated obstructive pulmonary disease (SPAOPD) was determined. TBLF was collected during the summer (July) and winter (February) seasons. The serum/TBLF urea nitrogen ratio was used to standardize the mediator concentration in the TBLF. Four strips were used from each guinea-pig. The first strip did not receive any antagonist and served as the control. The second, third and fourth strips received antagonists of PGE2, LTD4 and PAF, respectively at 10(-6) mol/L for 30 min. The tissues were then precontracted with a dose of histamine (10(-5) mol/L) and their responses to 1 ml of TBLF were determined. The study showed that TBLF obtained in the summer from unaffected horses produced a significantly greater relaxation than that from the affected horses, whereas TBLF obtained in the winter from unaffected or affected horses did not cause a significantly different degree of relaxation. Among the antagonist-treated strips, only those exposed to the PGE2 blocker showed a significant reduction in the relaxation caused by TBLF obtained in the summer from SPAOPD horses. This suggests that PGE2 is an important mediator present in the summer in the TBLF from horses affected with SPAOPD.
Publication Date: 1998-12-30 PubMed ID: 9868763DOI: 10.1023/a:1006131217140Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research focuses on how guinea-pigs’ lung tissue reacts to a fluid extracted from horses suffering from a specific lung disease. The results suggest that a certain agent, known as PGE2, plays an integral role in this reaction, especially during the summer season.
Objective of the Study
- The main purpose of this research was to understand the reaction of lung tissues (referred to as parenchymal strips) from guinea-pigs when exposed to tracheobronchial lavage fluid (TBLF), sourced from both normal horses and those suffering from a condition known as summer pasture-associated obstructive pulmonary disease (SPAODP).
Methodology
- TBLF was collected from a total of 16 horses, 8 of which were healthy, and 8 that were suffering from SPAOPD during the summer (July) and winter (February) seasons.
- To standardize the concentration of bioactive substances or mediators in the TBLF, the researchers used the serum/TBLF urea nitrogen ratio.
- Four strips of lung tissue were used from each guinea-pig. The first strip acted as a control and received no treatment. The other three strips were treated with various antagonists of PGE2, LTD4, and PAF substances, to block their action.
- The lung tissues were first precontracted with histamine and their response to TBLF was then evaluated.
Findings
- The research found that the TBLF obtained in summer from healthy horses caused a significantly higher relaxation response in the guinea pig’s lung tissue compared to that from SPAOPD-affected horses.
- However, TBLF gathered in winter from either healthy or affected horses didn’t cause significantly different relaxation responses.
- Amongst all the tissues treated with antagonists, those exposed to the PGE2 blocker showed a marked reduction in the relaxation response to TBLF obtained in summer from SPAOPD-affected horses.
Conclusion
- The results of the study indicate that PGE2 is an influential mediator in tracheobronchial lavage fluid taken from horses affected with SPAOPD, especially during the summer. It significantly impacts the relaxation response in the guinea pig’s lung tissue when exposed to this fluid.
Cite This Article
APA
Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP.
(1998).
Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease.
Vet Res Commun, 22(7), 493-503.
https://doi.org/10.1023/a:1006131217140 Publication
Researcher Affiliations
- Department of Veterinary Physiology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
MeSH Terms
- Animal Feed / adverse effects
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Bronchoalveolar Lavage Fluid
- Dinoprostone / antagonists & inhibitors
- Guinea Pigs
- Histamine / pharmacology
- Horse Diseases / blood
- Horse Diseases / etiology
- Horse Diseases / physiopathology
- Horses
- In Vitro Techniques
- Leukotriene D4 / antagonists & inhibitors
- Lung / drug effects
- Lung / physiology
- Lung Diseases, Obstructive / etiology
- Lung Diseases, Obstructive / physiopathology
- Lung Diseases, Obstructive / veterinary
- Muscle Contraction / drug effects
- Muscle Relaxation / drug effects
- Muscle, Smooth / drug effects
- Muscle, Smooth / physiology
- Phospholipid Ethers / pharmacology
- Platelet Activating Factor / antagonists & inhibitors
- Platelet Aggregation Inhibitors / pharmacology
- Poaceae / adverse effects
- Prostaglandin Antagonists / pharmacology
- Quinolines / pharmacology
- Reference Values
- Seasons
- Trachea / physiology
- Trachea / physiopathology
- Xanthenes / pharmacology
- Xanthones
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