Restriction endonuclease analysis of equine herpesvirus-1 isolates recovered in Ontario, 1986-1992, from aborted, stillborn, and neonatal foals.
Abstract: Ninety-two equine herpesvirus type 1 isolates were recovered from aborted, stillborn, or neonatal foals from Ontario, Canada, from 1986 to 1992. From this total, 32 strains were randomly chosen for further study. Four or 5 isolates from each winter were selected, each from a different premises, and characterized by restriction enzyme analysis using BamHI, KpnI, BglII, HindIII, and EcoRI. Additional isolates from 2 premises and from a zebra foal were also assessed. For the strains isolated in 1986 and 1989-1992, the DNA pattern of 18 strains was similar to that of type 1P (Kentucky D) for BamHI and KpnI. None of the 32 strains studied could be differentiated by HindIII or EcoRI. Using BglII, an inconsistent fragment pattern and distribution were observed. Of the 8 strains isolated in 1987 and 1988, 7 were assigned into the 1B prototype group. The geographic distribution of 17 type 1P and 12 1B isolates was random across southern Ontario. These findings suggest that both electropherotypes can be recovered from horses in Ontario. The patterns of the additional equine isolates from the same premises were identical. The zebra isolate was different from the prototype equine herpesvirus type 1 and type 4 patterns and from all other equine isolates.
Publication Date: 1997-04-01 PubMed ID: 9211232DOI: 10.1177/104063879700900206Google Scholar: Lookup
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- Journal Article
Summary
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The research paper examines the specific characteristics of 92 strains of equine herpesvirus type 1 recovered from foals in Ontario, Canada between 1986 and 1992, highlighting how these strains differ by their DNA patterns produced by various restriction enzymes.
Selection and Analysis of Virus Strains
- The researchers analyzed 92 samples of equine herpesvirus type 1 isolated from aborted, stillborn, or neonatal foals in Ontario.
- From these, 32 strains were randomly selected for deeper inspection. Four or five strains from different premises each winter were chosen.
- The chosen samples were analyzed using restriction enzyme analysis with several enzymes, namely BamHI, KpnI, BglII, HindIII, and EcoRI.
- Additional isolates were also evaluated from two other premises and from a zebra foal.
Findings from DNA Pattern Analysis
- The DNA pattern of 18 strains, isolated in 1986 and from 1989 to 1992, similar to that of type 1P (Kentucky D) for BamHI and KpnI enzymes.
- HindIII or EcoRI enzymes couldn’t differentiate any of the 32 examined strains.
- An inconsistent fragment pattern and distribution were discovered when using BglII.
- Of the 8 strains identified in 1987 and 1988, 7 were grouped into the 1B prototype group.
Geographic Distribution and Special Cases
- Seventeen type 1P and twelve 1B isolates were distributed randomly across southern Ontario, indicating both electropherotypes can be found in the region.
- The patterns of additional equine isolates from the same premises were found identical.
- A standout finding was the zebra isolate, which differed from the standard equine herpesvirus type 1 and type 4 patterns and from all equine isolates.
The research lends insight into the diversity and behavior of equine herpesvirus type 1 and its geographical dispersion. Based on their DNA patterns, these isolates can be differentiated, which can potentially aid in future studies and disease management strategies.
Cite This Article
APA
Nagy E, Idamakanti N, Carman S.
(1997).
Restriction endonuclease analysis of equine herpesvirus-1 isolates recovered in Ontario, 1986-1992, from aborted, stillborn, and neonatal foals.
J Vet Diagn Invest, 9(2), 143-148.
https://doi.org/10.1177/104063879700900206 Publication
Researcher Affiliations
- Department of pathobiology, Ontario Veterinary College, University of Guelph, Canada.
MeSH Terms
- Abortion, Veterinary / virology
- Animals
- Animals, Newborn
- Cell Line
- DNA Restriction Enzymes
- DNA, Viral / analysis
- Female
- Fetal Death
- Fetus / virology
- Geography
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 1, Equid / classification
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases
- Horses
- Ontario
- Pregnancy
- Pregnancy Complications, Infectious / veterinary
- Pregnancy Complications, Infectious / virology
- Restriction Mapping
Citations
This article has been cited 2 times.- Rebelo AR, Carman S, Shapiro J, van Dreumel T, Hazlett M, Nagy É. Equid herpesvirus 9 (EHV-9) isolates from zebras in Ontario, Canada, 1989 to 2007. Can J Vet Res 2015 Apr;79(2):155-9.
- Brash ML, Nagy É, Pei Y, Carman S, Emery S, Smith AE, Turner PV. Acute hemorrhagic and necrotizing pneumonia, splenitis, and dermatitis in a pet rabbit caused by a novel herpesvirus (leporid herpesvirus-4). Can Vet J 2010 Dec;51(12):1383-6.
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