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Archives of virology1983; 77(2-4); 249-258; doi: 10.1007/BF01309272

Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1.

Abstract: DNA was prepared from 43 equine herpesvirus type 1 (EHV 1) isolates, 11 of which were from horses with respiratory disease, 22 from aborted equine foetuses, and 10 from foals that died perinatally. The restriction endonuclease DNA fingerprints of 10 of the 11 respiratory isolates, known with certainty to have been recovered from horses with respiratory disease, were entirely different from all but 3 of the 32 foetal or perinatal foal isolates. The exceptional respiratory isolate, EHV 1 Army 183, had a foetal (F) strain fingerprint but this virus cannot be said with certainty to have been isolated from the respiratory tract. The 3 exceptional foetal isolates, had respiratory (R) strain fingerprints, and were recovered from single sporadic abortions. There are no exceptions to the view that only R strains have been isolated from naturally occurring respiratory disease. Also it is clear that major epizootics of abortion (abortion storms) and of perinatal foal mortality are caused by F strains. The data together with an analysis of the epidemiological patterns, particularly in Australia, strongly support the view that F and R strains be regarded as separate species, EHV 1 and 4 respectively.
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Publication Date: 1983-01-01 PubMed ID: 6314938DOI: 10.1007/BF01309272Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the differences in DNA composition of various strains of equine herpesvirus type 1 (EHV 1), separated into those caused respiratory disease in horses, those that caused abortion in horse fetuses, and those that led to death in foals born around their due date. The results suggest that these varying forms of EHV 1 might in fact be separate species.

DNA Collection and Analysis

  • The researchers prepared DNA from 43 isolates of EHV 1, with each isolate coming from a different group of horses – 11 from horses with respiratory disease, 22 from aborted horse fetuses and 10 from foals that died around their due date.
  • They then compared the DNA “fingerprints” of these strains to observe any noticeable differences.

Divergent DNA Fingerprints

  • The DNA fingerprints recorded for 10 out of 11 respiratory isolates were different from those found in all but 3 of the 32 fetal or newborn foal isolates. The fingerprints in this context refers to the unique patterns of DNA fragments produced by restriction endonucleases, enzymes that cut DNA at specific sequences.
  • One respiratory isolate, EHV 1 Army 183, unexpectedly had a foetal strain fingerprint, but it wasn’t confirmed whether this virus was isolated from the respiratory tract, adding an element of uncertainty to the observation.
  • Additionally, three of the foetal isolates had respiratory strain fingerprints, though these strains were recovered from isolated, sporadic abortions, making these exceptions noteworthy.

Suggestions for Strain Classification

  • Within their findings, there was no deviation from the belief that only respiratory (R) strains have been isolated from naturally occurring respiratory disease.
  • Moreover, it appeared that major outbreaks of horse abortion and perinatal foal death were caused exclusively by foetal (F) strains of EHV 1.
  • Basing on the data and the analysis of associated disease patterns, particularly in Australia, the researchers strongly recommend that F and R strains should be considered as separate species, redesignated as EHV 1 and 4, respectively.

Cite This Article

APA
Studdert MJ. (1983). Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. Arch Virol, 77(2-4), 249-258. https://doi.org/10.1007/BF01309272

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 77
Issue: 2-4
Pages: 249-258

Researcher Affiliations

Studdert, M J

    MeSH Terms

    • Abortion, Veterinary / microbiology
    • Animals
    • Bacterial Proteins
    • Base Sequence
    • DNA Restriction Enzymes
    • DNA, Viral / analysis
    • Deoxyribonuclease BamHI
    • Deoxyribonuclease EcoRI
    • Deoxyribonucleases, Type II Site-Specific
    • Female
    • Fetus / microbiology
    • Herpesviridae / analysis
    • Herpesviridae Infections / microbiology
    • Herpesviridae Infections / veterinary
    • Herpesvirus 1, Equid / analysis
    • Herpesvirus 1, Equid / isolation & purification
    • Horse Diseases / microbiology
    • Horses / microbiology
    • Pregnancy
    • Respiratory Tract Infections / microbiology
    • Respiratory Tract Infections / veterinary

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    Citations

    This article has been cited 12 times.
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    2. Cleri DJ, Ricketti AJ, Porwancher RB, Ramos-Bonner LS, Vernaleo JR. Viral hemorrhagic fevers: current status of endemic disease and strategies for control. Infect Dis Clin North Am 2006 Jun;20(2):359-93, x.
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    4. Crabb BS, Studdert MJ. Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host. J Virol 1993 Oct;67(10):6332-8.
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    5. Bouchey D, Evermann J, Jacob RJ. Molecular pathogenesis of equine coital exanthema (ECE): temperature sensitivity (TS) and restriction endonuclease (RE) fragment profiles of several field isolates. Arch Virol 1987;92(3-4):293-9.
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