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BMC research notes2016; 9; 353; doi: 10.1186/s13104-016-2161-9

RNA expression of TLR10 in normal equine tissues.

Abstract: Toll like receptors are one of the major innate immune system pathogen recognition systems. There is little data on the expression of the TLR10 member of this family in the horse. Results: This paper describes the genetic structure of the Equine TLR10 gene and its RNA expression in a range of horse tissues. It describes the phylogenetic analysis of the Equine TLR1,6,10,2 annotations in the horse genome, firmly identifying them in their corresponding gene clades compared to other species and firmly placing the horse gene with other TLR10 genes from odd-toed ungulates. Additional 3' transcript extensions to that annotated for TLR10 in the horse genome have been identified by analysis of RNAseq data. RNA expression of the equine TLR10 gene was highest in peripheral blood mononucleocytes and lymphoid tissue (lymph nodes and spleen), however some expression was detected in all tissues tested (jejunum, caudal mesenteric lymph nodes, bronchial lymph node, spleen, lung, colon, kidney and liver). Additional data on RNAseq expression of all equine TLR genes (1-4 and 6-10) demonstrate higher expression of TLR4 than other equine TLRs in all tissues. Conclusions: The equine TLR10 gene displays significant homology to other mammalian TLR10 genes and could be reasonably assumed to have similar fuctions. Its RNA level expression is higher in resting state PBMCs in horses than in other tissues.
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Publication Date: 2016-07-19 PubMed ID: 27435589PubMed Central: PMC4952062DOI: 10.1186/s13104-016-2161-9Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the RNA expression of TLR10 in different horse tissues, with the highest expression noted in peripheral blood mononucleocytes and lymphoid tissue. The result hints that the equine TLR10 gene may function in a similar manner to other mammalian TLR10 genes as it shows significant homology.

Background

  • The study aimed at understanding the role of Toll-like receptors (TLRs) in horses, especially TLR10, a member of the TLR family, for which there is limited knowledge. TLRs are crucial components of the innate immune system responsible for recognizing and responding to pathogens.

Results

  • The research details the Equine TLR10 gene’s genetic makeup and its RNA expression across various horse tissues. It provides insight into the horse genome concerning the Equine TLR1,6,10,2 through extensive phylogenetic analysis. This analysis confirmed their place within gene clades compared to other species, solidly placing the horse gene with other TLR10 genes from odd-toed ungulates.
  • Further analysis of RNAseq data revealed additional 3′ transcript extensions for TLR10 in the horse genome.
  • The study assessed the RNA expression of the equine TLR10 gene in multiple tissues including jejunum, caudal mesenteric lymph nodes, bronchial lymph node, spleen, lung, colon, kidney, and liver. The highest RNA expression levels were observed in peripheral blood mononucleocytes and lymphoid tissues such as lymph nodes and spleen. A degree of expression was detected in all tissues tested.
  • The RNAseq expression data for all equine TLR genes (including 1-4 and 6-10) demonstrates higher expression of TLR4 than other equine TLRs in all tissues.

Conclusion

  • The study concluded that the equine TLR10 gene is significantly similar to other mammalian TLR10 genes. This suggests that it is likely to possess similar functions.
  • The study also found that RNA level expression of this gene is higher in a horse’s resting state PBMCs than in other tissues.

Cite This Article

APA
Tarlinton RE, Alder L, Moreton J, Maboni G, Emes RD, Tötemeyer S. (2016). RNA expression of TLR10 in normal equine tissues. BMC Res Notes, 9, 353. https://doi.org/10.1186/s13104-016-2161-9

Publication

BMC research notes
ISSN: 1756-0500
NlmUniqueID: 101462768
Country: England
Language: English
Volume: 9
Pages: 353
PII: 353

Researcher Affiliations

Tarlinton, Rachael E
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK. rachael.tarlinton@nottingham.ac.uk.
Alder, Lauren
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
Moreton, Joanna
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
  • Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
Maboni, Grazieli
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
Emes, Richard D
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
  • Advanced Data Analysis Centre, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
Tötemeyer, Sabine
  • School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

MeSH Terms

  • Animals
  • Gene Expression
  • Genome
  • Horses / classification
  • Horses / genetics
  • Horses / immunology
  • Immunity, Innate
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Phylogeny
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • RNA, Messenger / genetics
  • RNA, Messenger / immunology
  • Sequence Analysis, RNA
  • Sequence Homology, Amino Acid
  • Spleen / immunology
  • Spleen / metabolism
  • Toll-Like Receptor 10 / genetics
  • Toll-Like Receptor 10 / immunology

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Citations

This article has been cited 6 times.
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