Role of PGF2α in luteolysis based on inhibition of PGF2α synthesis in the mare.
Abstract: The effects of inhibition of PGF2α synthesis on luteolysis in mares and on the incidence of prolonged luteal activity were studied in controls and in a group treated with flunixin meglumine (FM), a PGF2α inhibitor (n = 6/group). The FM was given every 8 hours (1.0 mg/kg) on each of Days 14.0 to 16.7. Concentration (pg/mL) of PGF2α metabolite averaged over 8 hours of hourly blood sampling at the beginning of each day, was lower in the FM group than in the controls on Day 14 after ovulation (6.7 ± 1.3 vs. 13.8 ± 2.9, P < 0.05), Day 15 (15.0 ± 3.9 vs. 35.2 ± 10.4, P < 0.10), and Day 16 (21.9 ± 5.7 vs. 54.7 ± 11.4, P < 0.03). Concentration (ng/mL) of progesterone (P4) was greater in the FM group than in the controls on Day 14 (10.1 ± 0.9 vs. 7.7 ± 0.9, P < 0.08), Day 15 (9.2 ± 1.0 vs. 4.3 ± 1.0, P < 0.008), and Day 16 (5.6 ± 1.6 vs. 1.2 ± 0.4, P < 0.02). The interval from ovulation to the beginning of a decrease in P4 and to the end of luteolysis (P4 < 1 ng/mL) was each delayed (P < 0.03) by ~1 day in the FM group. Intervals involving the luteal phase were long (statistical outliers, P < 0.05) in two mares in the FM group, indicating prolonged luteal activity. Results supported the hypotheses that (1) inhibition of PGF2α synthesis interferes with luteolysis in mares and (2) inhibition of PGF2α at the expected time of luteolysis may lead to prolonged luteal activity.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication Date: 2013-08-13 PubMed ID: 23953743DOI: 10.1016/j.theriogenology.2013.07.008Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the effects of inhibiting PGF2α synthesis, with a specific compound – flunixin meglumine, on the process of luteolysis (degradation of the corpus luteum) and the occurrence of prolonged luteal activity in mares. The results suggest that interference with PGF2α synthesis disrupts luteolysis and its inhibition at certain stages could lead to prolonged luteal activity.
Study Design and Implementation
- The study was designed to assess the impact of inhibiting the synthesis of PGF2α, a key hormone involved in the reproductive cycle of mares. The inhibition was executed using a compound known as flunixin meglumine (FM).
- Two groups of mares formed the study sample. One group was the control group (no flunixin meglumine treatment), and the other group was treated with FM. Each group consisted of six mares.
- The FM was administered every eight hours into the bodies of the mares in the treatment group from Day 14.0 to Day 16.7 of the mares’ reproductive cycle.
Observations and Measurements
- Blood samples were taken repeatedly at hourly intervals at the start of each day of the treatment and the concentrations of PGF2α and progesterone (P4) were measured.
- The study found that the concentration of PGF2α was significantly lower in the FM-treated group compared with the control group on Days 14, 15, and 16 post-ovulation. Correspondingly, the concentration of progesterone was found to be higher in the FM-treated group on these same days.
- Additionally, the study also noted a delay in the decrease of P4 levels and the end of luteolysis in the FM-treated group compared to the control group.
Key Conclusions
- Through these measurements and comparisons, the researchers concluded that inhibiting PGF2α synthesis interferes with the process of luteolysis in mares.
- The study also suggests that blocking PGF2α at the expected time of luteolysis could lead to prolonged luteal activity, as evidenced by two mares in the FM-treated group showing statistically significant prolonged luteal phases.
Cite This Article
APA
Santos VG, Beg MA, Bettencourt EM, Ginther OJ.
(2013).
Role of PGF2α in luteolysis based on inhibition of PGF2α synthesis in the mare.
Theriogenology, 80(7), 812-820.
https://doi.org/10.1016/j.theriogenology.2013.07.008 Publication
Researcher Affiliations
- Eutheria Foundation, Cross Plains, Wisconsin, USA; ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Department of Veterinary Medicine, Escola de Ciências e Tecnologia, Universidade de Évora, Núcleo da Mitra, Évora, Portugal; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
MeSH Terms
- Animals
- Clonixin / analogs & derivatives
- Clonixin / pharmacology
- Dinoprost / metabolism
- Dinoprost / physiology
- Estrous Cycle / drug effects
- Estrous Cycle / metabolism
- Female
- Horses / metabolism
- Horses / physiology
- Luteolysis / drug effects
- Luteolysis / physiology
- Progesterone / blood
- Prostaglandin Antagonists / pharmacology
- Time Factors
Citations
This article has been cited 1 times.- Schlapp G, Goyeneche L, Fernández G, Menchaca A, Crispo M. Administration of the nonsteroidal anti-inflammatory drug tolfenamic acid at embryo transfer improves maintenance of pregnancy and embryo survival in recipient mice. J Assist Reprod Genet 2015 Feb;32(2):271-5.
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