Scanning electron microscope observations of hoof horn from horses with brittle feet.

This is a study on the microscopic examination of hoof clippings from horses with brittle feet, identifying two types of defects and the impact of biotin treatment and limestone addition to the diet on these defects.

Objective of the Study

• The aim of this study was to examine, using a scanning electron microscope, the hoof clippings from horses with brittle feet to identify any potential defects on a microscopic scale. The research also aimed to evaluate the effects of biotin treatment and the inclusion of powdered limestone in the horse’s diet on these defects.

Observations and Findings

• Through scanning electron microscope, the researchers observed two types of defects in the hoof clippings of horses suffering from brittle feet.
• The first defect displayed a loss of structure and horn in the stratum externum, which is the horse’s outermost layer of the hoof. This defect responded favorably to biotin treatment, showing an apparent restoration of the hoof’s structure.
• The second defect showed poor attachment of the horn squames, structures that contribute to the toughness of the horse’s hoof. This defect did not respond to biotin treatment.

Impact of Dietary Adjustments

• The researchers then evaluated the effect of additional dietary changes on this second defect. The study found that incorporating powdered limestone into the horses’ diet brought about an improvement in this defect.
• This suggests that horses’ diet, specifically the deficiency of some nutrients, might play a significant role in hoof health. Further suggesting that dietary adjustment might be an effective approach in addressing certain hoof defects.

Conclusion and Implications

• The study provides valuable insight into the microscopic characteristics of brittle feet in horses, highlighting two specific defects that can contribute to the condition.
• This research is significant in that it outlines potential treatment options for horses with brittle feet, including biotin treatment and dietary adjustments with the inclusion of powdered limestone. This could open avenues for further research into the role of diet and specific nutrients in animal hoof health.