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Home / Equine Research Bank / J Reprod Dev / Season does not have a deleterious effect on proportions of ...
The Journal of reproduction and development2020; 66(3); 215-221; doi: 10.1262/jrd.2019-072

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins.

Abstract: The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.
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Publication Date: 2020-02-13 PubMed ID: 32051351PubMed Central: PMC7297632DOI: 10.1262/jrd.2019-072Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates seasonal changes in the protein composition of stallion semen and its impact on sperm quality. The findings suggest that there are no significant seasonal differences in seminal plasma proteins and sperm viability, making it possible to collect and freeze stallion semen throughout the year.

Understanding the Research

  • The study aimed to understand how the composition of seminal plasma proteins in stallion semen changes across breeding and non-breeding seasons. Seminal plasma proteins perform crucial functions that facilitate fertilization, and any temporal alterations in these proteins play a role in the overall quality and fertility of the semen.
  • The researchers analyzed the levels of three protein groups in the seminal plasma: non-heparin-binding proteins, phosphorylcholine-binding proteins, and heparin-binding proteins. They also assessed the sperm quality based on several criteria, such as sperm kinematics, sperm morphology, mitochondrial membrane potential, the production of reactive oxygen species, chromatin integrity, and testosterone levels.

Findings of the Research

  • The study found negligible differences in the major protein groups between the breeding and non-breeding seasons. It observed a slightly higher level of non-heparin binding proteins and heparin-binding proteins in the non-breeding season, but these variations were insignificant.
  • Examination of the semen characteristics presented a different scenario. While the morphological attributes of the sperm remained relatively stable across seasons, the measurement of mitochondrial membrane potential and the production of reactive oxygen species showed lower sperm quality during the breeding season. However, the chromatin integrity and testosterone levels remained consistent irrespective of the season.

Implications of the Study

  • This study suggests that seasonality does not significantly affect the protein composition of stallion semen and, consequently, sperm quality. Thus, stallion ejaculates are suitable for freezing and utilization throughout the year.
  • While there were minor seasonal variations in sperm quality, these changes did not impair the sperm’s function during the non-breeding season. This finding supports the year-round collection and freezing of stallion semen, opening up new opportunities in equine breeding and fertility practices.

Cite This Article

APA
Johannisson A, Al-Essawe EM, Al-Saffar AK, Karkehabadi S, Lima-Verde I, Wulf M, Aurich C, Morrell JM. (2020). Season does not have a deleterious effect on proportions of stallion seminal plasma proteins. J Reprod Dev, 66(3), 215-221. https://doi.org/10.1262/jrd.2019-072

Publication

The Journal of reproduction and development
ISSN: 1348-4400
NlmUniqueID: 9438792
Country: Japan
Language: English
Volume: 66
Issue: 3
Pages: 215-221

Researcher Affiliations

Johannisson, Anders
  • Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, Sweden.
Al-Essawe, Essraa M
  • Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, Sweden.
  • Al-Nahrain University-High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Clinical Reproductive Physiology, Baghdad 10007, Iraq.
Al-Saffar, Anas Kh
  • Gastroenterology and Hepatology Unit, Department of Medical Sciences, Uppsala University, Uppsala 75185, Sweden.
  • Department of Surgery and Obstetrics, College of Veterinary Medicine, Baghdad University, Baghdad 10081, Iraq.
Karkehabadi, Saeid
  • Swedish University of Agricultural Sciences, Molecular Sciences, Uppsala 75007, Sweden.
Lima-Verde, Isabel
  • Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, Sweden.
Wulf, Manuela
  • Graf Lehndorff Institute for Equine Sciences, Vetmeduni, Vienna, Austria, and Neustadt (Dosse) 16845, Germany.
Aurich, Christine
  • Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna 1210, Austria.
Morrell, Jane M
  • Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala 75007, Sweden.

MeSH Terms

  • Animals
  • Horses
  • Male
  • Membrane Potential, Mitochondrial / physiology
  • Reactive Oxygen Species / metabolism
  • Seasons
  • Semen Analysis / veterinary
  • Semen Preservation
  • Seminal Plasma Proteins / metabolism
  • Sperm Motility / physiology
  • Spermatozoa / metabolism

Conflict of Interest Statement

The authors do not have any conflicts of interest to declare.

References

This article includes 30 references
  1. Kareskoski M, Katila T. Components of stallion seminal plasma and the effects of seminal plasma on sperm longevity.. Anim Reprod Sci 2008 Sep;107(3-4):249-56.
    pubmed: 18556156doi: 10.1016/j.anireprosci.2008.04.013google scholar: lookup
  2. Calvete JJ, Nessau S, Mann K, Sanz L, Sieme H, Klug E, Töpfer-Petersen E. Isolation and biochemical characterisation of stallion seminal-plasma proteins.. Reprod Domest Anim 1994; 29: 411–426.
  3. Calvete JJ, Raida M, Gentzel M, Urbanke C, Sanz L, Töpfer-Petersen E. Isolation and characterization of heparin- and phosphorylcholine-binding proteins of boar and stallion seminal plasma. Primary structure of porcine pB1.. FEBS Lett 1997 Apr 28;407(2):201-6.
    pubmed: 9166899doi: 10.1016/s0014-5793(97)00344-xgoogle scholar: lookup
  4. Kareskoski AM, del Alamo MM, Güvenc K, Reilas T, Calvete JJ, Rodriguez-Martinez H, Andersson M, Katila T. Protein composition of seminal plasma in fractionated stallion ejaculates.. Reprod Domest Anim 2011 Feb;46(1):e79-84.
    pubmed: 20546178doi: 10.1111/j.1439-0531.2010.01641.xgoogle scholar: lookup
  5. Love CC, Brinsko SP, Rigby SL, Thompson JA, Blanchard TL, Varner DD. Relationship of seminal plasma level and extender type to sperm motility and DNA integrity.. Theriogenology 2005 Apr 1;63(6):1584-91.
    pubmed: 15763103doi: 10.1016/j.theriogenology.2004.05.030google scholar: lookup
  6. Morrell JM, Pihl J, Dalin AM, Johannisson A. Restoration of seminal plasma to stallion spermatozoa selected by colloid centrifugation increases sperm progressive motility but is detrimental to chromatin integrity.. Theriogenology 2012 Jul 15;78(2):345-52.
    pubmed: 22494676doi: 10.1016/j.theriogenology.2012.02.009google scholar: lookup
  7. Kareskoski M, Sankari S, Johannisson A, Kindahl H, Andersson M, Katila T. The association of the presence of seminal plasma and its components with sperm longevity in fractionated stallion ejaculates.. Reprod Domest Anim 2011 Dec;46(6):1073-81.
    pubmed: 21535237doi: 10.1111/j.1439-0531.2011.01789.xgoogle scholar: lookup
  8. Vidament M, Dupere AM, Julienne P, Evain A, Noue P, Palmer E. Equine frozen semen: freezability and fertility field results.. Theriogenology 1997 Oct 15;48(6):907-17.
    pubmed: 16728182doi: 10.1016/s0093-691x(97)00319-1google scholar: lookup
  9. Mráčková M, Zavadilová M, Sedlinska M. Assessment of the effect of selected components of equine seminal plasma on semen freezability.. Maced Vet Rev 2015; 38: 91–96.
  10. Usuga A, Rojano B, Restrepo B, Restrepo G. Effect of seminal plasma components on the quality of fresh and cryopreserved stallion semen.. J Eq Vet Sci 2017; 58: 103–111.
  11. Usuga A, Rojano BA, Restrepo G. Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen.. Reprod Fertil Dev 2018 Mar;30(3):563-569.
    pubmed: 28855043doi: 10.1071/rd17044google scholar: lookup
  12. Wach-Gygax L, Burger D, Malama E, Bollwein H, Fleisch A, Jeannerat E, Thomas S, Schuler G, Janett F. Seasonal changes of DNA fragmentation and quality of raw and cold-stored stallion spermatozoa.. Theriogenology 2017 Sep 1;99:98-104.
    pubmed: 28708505doi: 10.1016/j.theriogenology.2017.05.025google scholar: lookup
  13. Janett F, Thun R, Niederer K, Burger D, Hässig M. Seasonal changes in semen quality and freezability in the Warmblood stallion.. Theriogenology 2003 Aug;60(3):453-61.
    pubmed: 12763159doi: 10.1016/s0093-691x(03)00046-3google scholar: lookup
  14. Aurich C. Reprint of: Seasonal influences on cooled-shipped and frozen-thawed stallion semen.. J Eq Vet Sci 2016; 43: S1–S5.
  15. Al-Essawe EM, Johannisson A, Wulf M, Aurich C, Morrell JM. Improved cryosurvival of stallion spermatozoa after colloid centrifugation is independent of the addition of seminal plasma.. Cryobiology 2018 Apr;81:145-152.
    pubmed: 29397923doi: 10.1016/j.cryobiol.2018.01.009google scholar: lookup
  16. Varilová T, Seménková H, Horák P, Madera M, Pacáková V, Tichá M, Stulík K. Affinity liquid chromatography and capillary electrophoresis of seminal plasma proteins.. J Sep Sci 2006 May;29(8):1110-5.
    pubmed: 16830725doi: 10.1002/jssc.200500405google scholar: lookup
  17. Madej M, Norrby M, Madsen M, Johannisson A, Hansen C, Madej A. The effect of boar seminal plasma on the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells.. Reprod Domest Anim 2012 Feb;47(1):113-24.
    pubmed: 21615801doi: 10.1111/j.1439-0531.2011.01809.xgoogle scholar: lookup
  18. Lagerlöf N. Changes in the spermatozoa and in the testes of bulls with impaired or enhanced fertility.. Acta Pathol Microbiol Scand 1934; (Supplement 19): 254–267.
  19. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.. J Androl 2002 Jan-Feb;23(1):25-43.
    pubmed: 11780920doi: 10.1002/j.1939-4640.2002.tb02599.xgoogle scholar: lookup
  20. Johannisson A, Morrell JM, Thorén J, Jönsson M, Dalin AM, Rodriguez-Martinez H. Colloidal centrifugation with Androcoll-E prolongs stallion sperm motility, viability and chromatin integrity.. Anim Reprod Sci 2009 Nov;116(1-2):119-28.
    pubmed: 19217226doi: 10.1016/j.anireprosci.2009.01.008google scholar: lookup
  21. Restrepo G, Rojano B, Usuga A. Relationship of cysteine-rich secretory protein-3 gene and protein with semen quality in stallions.. Reprod Domest Anim 2019 Jan;54(1):39-45.
    pubmed: 30099787doi: 10.1111/rda.13309google scholar: lookup
  22. Magdaleno L, Gasset M, Varea J, Schambony AM, Urbanke C, Raida M, Töpfer-Petersen E, Calvete JJ. Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family.. FEBS Lett 1997 Dec 29;420(2-3):179-85.
    pubmed: 9459306doi: 10.1016/s0014-5793(97)01514-7google scholar: lookup
  23. Novak S, Smith TA, Paradis F, Burwash L, Dyck MK, Foxcroft GR, Dixon WT. Biomarkers of in vivo fertility in sperm and seminal plasma of fertile stallions.. Theriogenology 2010 Oct 1;74(6):956-67.
    pubmed: 20580075doi: 10.1016/j.theriogenology.2010.04.025google scholar: lookup
  24. Brandon CI, Heusner GL, Caudle AB, Fayrer-Hosken RA. Two-dimensional polyacrylamide gel electrophoresis of equine seminal plasma proteins and their correlation with fertility.. Theriogenology 1999 Oct 1;52(5):863-73.
    pubmed: 10735126doi: 10.1016/s0093-691x(99)00178-8google scholar: lookup
  25. Rungruangsak J, Suwimonteerabutr J, Asawakarn B, Buranaamnumay K, Chantaravisoot N, Pisitkun T, Tummaruk P. Difference of seminal plasma proteins in good- and poor-freezability boar ejaculates.. Reprod Fertil Dev 2018; 30: 165–165.
  26. Fedorka CE, Scoggin KE, Woodward EM, Squires EL, Ball BA, Troedsson M. The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis.. Reprod Domest Anim 2017 Feb;52(1):89-96.
    pubmed: 27686063doi: 10.1111/rda.12813google scholar: lookup
  27. Aurich C, Gerlach T, Hoppen H-O, Aurich JE. Sexual activity influences the secretion of reproductive hormones in the stallion.. Reprod Domest Anim 1999; 34: 405–411.
  28. Blottner S, Warnke C, Tuchscherer A, Heinen V, Torner H. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season.. Anim Reprod Sci 2001 Jan 31;65(1-2):75-88.
    pubmed: 11182510doi: 10.1016/s0378-4320(00)00214-1google scholar: lookup
  29. Janett F, Thun R, Bettschen S, Burger D, Hassig M. Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.. Anim Reprod Sci 2003 Jul 15;77(3-4):213-21.
    pubmed: 12695055doi: 10.1016/s0378-4320(03)00039-3google scholar: lookup
  30. Morrell JM, Nongbua T, Valeanu S, Lima Verde I, Lundstedt-Enkel K, Edman A, Johannisson A. Sperm quality variables as indicators of bull fertility may be breed dependent.. Anim Reprod Sci 2017 Oct;185:42-52.
    pubmed: 28811063doi: 10.1016/j.anireprosci.2017.08.001google scholar: lookup

Citations

This article has been cited 2 times.
  1. Senra RL, Ramírez-López CJ, Magalhães-Júnior MJ, Neves JGDS, Barros E, Waddington B, Guimarães SEF, Guimarães JD, Baracat-Pereira MC. Kallikrein proteoforms and reproductive parameters in stallion are conditioned by climate. Sci Rep 2022 Nov 4;12(1):18690.
    doi: 10.1038/s41598-022-21350-wpubmed: 36333376google scholar: lookup
  2. Carvalho JC, Carvalho MG, Codognoto VM, Camargo LS, Kasimanickam R, Kastelic J, de Souza FF, Pinheiro Ferreira JC. Seasonal Changes in the Seminal Plasma Proteome of the Crab-Eating Fox (Cerdocyon thous). J Proteome Res 2026 Feb 6;25(2):723-734.
    doi: 10.1021/acs.jproteome.5c00694pubmed: 41474158google scholar: lookup
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