Selection of peptides for serological detection of equine infectious anemia.
Abstract: Equine infectious anemia caused by equine infectious anemia virus is an important disease due to its high severity and incidence in animals. We used a phage display library to isolate peptides that can be considered potential markers for equine infectious anemia diagnosis. We selected peptides using IgG purified from a pool comprised of 20 sera from animals naturally infected with equine infectious anemia virus. The diagnostic potential of these peptides was investigated by ELISA, Western blot and dot blot with purified IgG and serum samples. Based on the results, we chose a peptide mimetic for glycoprotein gp45 epitopes of equine infectious anemia virus, with potential for use as an antigen in indirect diagnostic assays. Synthesis of this peptide has possible applications for the development of new diagnostic tools for this disease.
Publication Date: 2012-08-13 PubMed ID: 22653674DOI: 10.4238/2012.May.24.2Google Scholar: Lookup
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- Journal Article
Summary
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This research article deals with the identification of peptides for the easier diagnosis of equine infectious anemia, a severe disease in animals. The peptides, which act as potential markers, were isolated using a phage display library, and their diagnostic potential was evaluated using various assays.
Isolation of peptides
- The researchers started their study by using a phage display library. This is a method where bacteriophages, viruses that infect bacteria, are used to express a variety of peptides on their surfaces.
- Through this technique, they isolated peptides that could be potential markers for the diagnosis of equine infectious anemia. These are indicative substances that could show if an animal is infected with the disease.
Selection of peptides
- The peptides were selected using IgG, a type of antibody, which was purified from a pool of 20 sera. The serum is the clotted part of the blood and the pool comprised of samples from animals naturally infected with the equine infectious anemia virus.
- This helps give their research relevance since the peptides were selected using real, naturally occurring samples.
Diagnostic potential of peptides
- The potential of these selected peptides for diagnosis was then evaluated using various assays including ELISA, Western blot, and dot blot.
- These are scientific methods used to detect the presence of antibodies or antigens in a sample. In this case, they helped determine how effective these peptides could be for diagnosing equine infectious anemia.
Peptide mimic for gp45 epitopes
- Based on the results, the researchers decided on a peptide that mimics the glycoprotein gp45 epitopes of the equine infectious anemia virus.
- Epitopes are the parts of an antigen that an antibody binds to. By choosing a peptide that mimics these epitopes, it’s possible to indirectly detect the virus – a useful tool for the diagnosis of the disease.
Application of the research
- Synthesis of this selected peptide could potentially be used in the development of new diagnostic tools for equine infectious anemia.
- This would help in faster and more efficient detection of the disease, aiding in the treatment and management of affected animals.
Cite This Article
APA
Santos EM, Cardoso R, Souza GR, Goulart LR, Heinemann MB, Leite RC, Reis JK.
(2012).
Selection of peptides for serological detection of equine infectious anemia.
Genet Mol Res, 11(3), 2182-2199.
https://doi.org/10.4238/2012.May.24.2 Publication
Researcher Affiliations
- Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. elmaira27@yahoo.com.br
MeSH Terms
- Amino Acid Sequence
- Animals
- Blotting, Western
- Computational Biology
- Enzyme-Linked Immunosorbent Assay
- Equine Infectious Anemia / blood
- Equine Infectious Anemia / diagnosis
- Horses / blood
- Horses / virology
- Immunoglobulin G / immunology
- Infectious Anemia Virus, Equine / isolation & purification
- Molecular Sequence Data
- Peptide Library
- Peptides / chemistry
Citations
This article has been cited 2 times.- Hurwitz AM, Huang W, Estes MK, Atmar RL, Palzkill T. Deep sequencing of phage-displayed peptide libraries reveals sequence motif that detects norovirus.. Protein Eng Des Sel 2017 Feb;30(2):129-139.
- Singha H, Goyal SK, Malik P, Khurana SK, Singh RK. Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus.. Indian J Virol 2013 Dec;24(3):349-56.
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