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The Canadian veterinary journal = La revue veterinaire canadienne2018; 59(9); 988-992;

Seminal plasma does not aid in the transport of phenolsulfonphthalein across the uterotubal junction in mares.

Abstract: This study tested the hypothesis that the presence of prostaglandin E2 in seminal plasma would aid in the transport of phenolsulfonphthalein (PSP) across the uterotubal junction. Five mares in estrus were inseminated during estrus with PSP dissolved in phosphate-buffered saline and during the subsequent estrus with PSP added to a standard insemination dose. Serum and urine samples were obtained at hours 0, 1, 2, and 3 following treatment and examined for the presence of PSP. Phenolsulfonphthalein could not be detected in any of the urine samples collected from mares following either treatment. None of the serum samples collected following intrauterine installation of PSP in PBS contained PSP. Phenolsulfonphthalein was detected in serum samples from 1 mare following insemination with semen containing PSP. Components in seminal plasma such as PGE2 did not facilitate the transport of PSP across the uterotubal junction as had been hypothesized. Cette étude a testé l’hypothèse voulant que la présence de la prostaglandine E2 dans le plasma séminal facilite le transport de la phénolsulfonphtaléine (PSP) au travers de la jonction utéro-tubaire. Cinq juments en oestrus ont été inséminées avec de la PSP dissoute dans une solution saline tamponnée au phosphate et, durant l’oestrus subséquent, avec de la PSP ajoutée à une dose d’insémination standard. Des prélèvements de sérum et d’urine ont été obtenus aux heures 0, 1, 2 et 3 ainsi qu’après le traitement et examinés pour déceler la présence de la PSP. La phénolsulfonphtaléine n’a pas pu être détectée dans aucun des échantillons d’urine prélevés auprès des juments après l’un ou l’autre des traitements. Aucun des échantillons de sérum prélevés après l’installation intra-utérine de la PSP dans PBS ne contenait de PSP. La phénolsulfonphtaléine a été détectée dans des échantillons de sérum provenant d’une jument après l’insémination avec du sperme contenant de la PSP. Des composants dans le plasma séminal comme le PGE2 n’ont pas facilité le transport de la PSP au travers de la jonction utéro-tubaire conformément à l’hypothèse émise.(Traduit par Isabelle Vallières).
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Publication Date: 2018-09-11 PubMed ID: 30197442PubMed Central: PMC6091138
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study has concluded that significant compounds in seminal plasma, such as prostaglandin E2, do not contribute to the transport of a test compound (phenolsulfonphthalein or PSP) across the uterotubal junction in mares.

Experiment Design

  • Five mares in estrus (a period of sexual receptivity in female horses) were selected as subjects for the study.
  • A compound known as phenolsulfonphthalein (PSP) was used as a marker compound and transported to the uterotubal junction via two methods – dissolved in a phosphate-buffered saline and incorporated within a standard insemination dose.
  • The timing was meticulous, with seminal doses being administered during the mares’ estrus period and subsequently during the next estrus period for the second method.

Data Collection

  • Serum and urine samples were collected at strategic hours immediately following treatment – at time (T) 0, 1, 2, and 3 hours post-treatment.
  • Examination of these samples was then carried out for the detection of PSP.

Research Findings

  • The study discovered that it was not possible to detect PSP in any of the urine samples originating from the mares after either treatment.
  • The same was true for serum samples collected after the treatment involving a solution of PSP in phosphate-buffered saline. No PSP was found.
  • However, PSP was detected in serum samples from one mare after insemination using semen containing PSP.

Conclusion

  • This led researchers to conclude that seminal plasma components, such as PGE2, did not facilitate the transport of PSP across the uterotubal junction as initially hypothesized.
  • This finding is likely to influence future understanding of uterotubal transport mechanisms and potentially reproductive biology in horses.

Cite This Article

APA
Ross KA, Kolb DS, Macedo A, Anderson M, Klein C. (2018). Seminal plasma does not aid in the transport of phenolsulfonphthalein across the uterotubal junction in mares. Can Vet J, 59(9), 988-992.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 59
Issue: 9
Pages: 988-992

Researcher Affiliations

Ross, Kayla A
  • University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).
Kolb, David S
  • University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).
Macedo, Alysson
  • University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).
Anderson, Marion
  • University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).
Klein, Claudia
  • University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).

MeSH Terms

  • Adnexal Diseases / diagnosis
  • Adnexal Diseases / veterinary
  • Animals
  • Dinoprostone
  • Estrus
  • Female
  • Horse Diseases / diagnosis
  • Horses
  • Insemination, Artificial / veterinary
  • Male
  • Oviducts / physiopathology
  • Phenolphthaleins / blood
  • Phenolphthaleins / urine
  • Phenolsulfonphthalein / administration & dosage
  • Phenolsulfonphthalein / analysis
  • Semen / chemistry

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Citations

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