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The Journal of veterinary medical science2017; 79(7); 1294-1300; doi: 10.1292/jvms.17-0133

Sensitive radioimmunoassay of total thyroxine (T4) in horses using a simple extraction method.

Abstract: Most thyroid hormone determinations in animals are based on immunoassays adapted from those used to test human samples, which may not reflect the actual values of thyroid hormone in horses because of the presence of binding proteins. The aims of the present study were i) to establish a novel radioimmunoassay (RIA) using a more simple and convenient method to separate binding proteins for the measurement of total thyroxine (T4) in horses and ii) to validate the assay by comparing total T4 concentrations in yearling horses raised in different climates. Blood samples were collected from trained yearlings in Hokkaido (temperate climate) and Miyazaki (subtropical climate) in Japan and from adult horses in estrus and diestrus. T4 was extracted from both serum and plasma using modified acid ethanol cryo-precipitation and sodium acetate ethanol methods. Circulating total T4 concentrations were determined by RIA. T4 concentration by sodium acetate ethanol was appropriately detectable rather than sodium salicylate method and was the same as for acid ethanol method. Furthermore, this sodium acetate ethanol method required fewer extraction steps than the other methods. Circulating T4 concentrations in yearlings were 225.98 ± 20.89 ng/ml, which was higher than the previous reference values. With respect to climate, T4 levels in Hokkaido yearlings tended to be higher than those in Miyazaki yearlings throughout the study period. These results indicated that this RIA protocol using a modified sodium acetate ethanol separation technique might be an appropriate tool for specific measurement of total T4 in horses.
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Publication Date: 2017-07-11 PubMed ID: 28603213PubMed Central: PMC5559379DOI: 10.1292/jvms.17-0133Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a study that develops and validates a simpler, more convenient method for measuring total thyroxine (T4), a thyroid hormone, in horses. Their method is a novel radioimmunoassay (RIA) that avoids problems with existing techniques focused on human samples that don’t account for binding proteins in animals. It also compares hormone concentrations in horses from different climatic conditions.

Research Objectives

  • The primary aim of this study was to establish a new radioimmunoassay (RIA) that uses a simpler, more accessible method to separate binding proteins when measuring total thyroxine (T4) levels in horses.
  • The second aim was to validate this new assay by comparing the total T4 concentrations observed in yearling horses raised in different climates.

Methodology

  • Blood samples were collected from trained yearlings in Hokkaido and Miyazaki in Japan, two areas with varying climates: temperate and subtropical, respectively. Samples were also taken from adult horses in estrus and diestrus.
  • The researchers separated T4 from both serum and plasma using modified acid ethanol cryo-precipitation and sodium acetate ethanol methods.
  • Circulating total T4 concentrations were then determined by the newly developed RIA.

Findings

  • T4 concentration was more detectable using the sodium acetate ethanol method compared to other methods. Additionally, this method required fewer extraction steps.
  • The study found that circulating T4 concentrations in yearlings were higher than previous reference values.
  • The researchers observed that T4 levels in Hokkaido yearlings were higher overall than those from Miyazaki yearlings throughout the duration of the study.

Conclusion

  • Based on their results, the researchers suggested that the RIA protocol they employed, featuring a modified sodium acetate ethanol separation technique, could be an appropriate tool for specifically measuring total T4 levels in horses.

Cite This Article

APA
Tangyuenyong S, Nambo Y, Nagaoka K, Tanaka T, Watanabe G. (2017). Sensitive radioimmunoassay of total thyroxine (T4) in horses using a simple extraction method. J Vet Med Sci, 79(7), 1294-1300. https://doi.org/10.1292/jvms.17-0133

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 79
Issue: 7
Pages: 1294-1300

Researcher Affiliations

Tangyuenyong, Siriwan
  • Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Nambo, Yasuo
  • Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-0834, Japan.
Nagaoka, Kentaro
  • Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Tanaka, Tomomi
  • Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
  • Laboratory of Veterinary Reproduction, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Watanabe, Gen
  • Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
  • Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.

MeSH Terms

  • Animals
  • Diestrus / blood
  • Estrus / blood
  • Female
  • Horses / blood
  • Male
  • Radioimmunoassay / methods
  • Radioimmunoassay / veterinary
  • Thyroxine / blood

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