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Sequence analyses of the p24 gene of Borna disease virus in naturally infected horse, donkey and sheep.
Abstract: By reverse transcriptase/PCR amplification and subsequent sequence determination of the p24 gene, the relatedness of Borna disease virus (BDV) in various naturally infected animal species was determined. These results are indicative of a common ancestral virus pool and a remarkably low species barrier of BDV. Comparison of 11 sequences to that of tissue culture adapted virus revealed that the homology among all isolates was at least 96.2% at the nucleotide level, and 97% at the amino acid level. Viral sequences from sheep, donkey and horse were found to be not more distantly related to each other than sequences from different infected horses. Tissue-specific virus variants were detected in one horse: the sequences established from infected cerebrum and kidney showed 10 mutations, whereas sequences obtained from parotid gland contained 20 mutations in comparison to the nucleotide sequence of MDCK cell adapted BDV.
Publication Date: 1994-12-01 PubMed ID: 7856315DOI: 10.1016/0168-1702(94)90128-7Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is about assessing the genetic relation of the Borna disease virus (BDV) across different naturally affected species – horses, sheep, and donkeys. It finds out that there is a strikingly low species barrier of BDV, and these species share a common ancestral virus pool.
Objective of the Study
This scientific study primarily aims to:
- Examine the relatedness of Borna disease virus (BDV) among different naturally affected species, namely horse, donkey, and sheep, by using the technique of reverse transcriptase Polymeric Chain Reaction (PCR) amplification and subsequent sequence determination of the p24 gene.
- Determine the genetic similarity of the BDV in these species at both the nucleotide and amino acid levels.
- Identify and contrast the tissue-specific variants of the virus within the same host species.
Methodology
The research has followed an elaborate methodology involving:
- Utilization of reverse transcriptase PCR for the amplification of the BDV sequences from the naturally-infected species.
- Post amplification, the sequence determination of the p24 gene, which is integral for BDV, was performed.
- The derived sequences of the virus from the different species were then put through comparison at both nucleotide and amino acid levels.
Findings of the Study
The findings of the research are manifold and provide significant insights into the genetic sequence and relatedness of BDV across different species.
- It was found that all the isolates had at least 96.2% homology at the nucleotide level and 97% at the amino acid level when compared with the tissue culture adapted virus.
- The sequences derived from the sheep, donkey, and horse were not more distantly related to each other than those from different infected horses.
- One horse showed tissue-specific virus variants: the sequences obtained from the infected cerebrum and kidney displayed 10 mutations, whereas sequences from the parotid gland showed 20 mutations when compared with the nucleotide sequence of the Madin-Darby Canine Kidney (MDCK) cell adapted BDV.
Conclusion
The study sheds light upon the highly similar nature of BDV in different species, indicating a remarkably low species barrier. The discovery of a common ancestral virus pool amongst these species prompts further research into the virus’ evolution and cross-species infectivity. The detection of tissue-specific virus mutations in the same host species is another significant finding that can steer future research.
Cite This Article
APA
Binz T, Lebelt J, Niemann H, Hagenau K.
(1994).
Sequence analyses of the p24 gene of Borna disease virus in naturally infected horse, donkey and sheep.
Virus Res, 34(3), 281-289.
https://doi.org/10.1016/0168-1702(94)90128-7 Publication
Researcher Affiliations
- Department of Microbiology, Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Borna Disease / virology
- Borna disease virus / genetics
- DNA, Viral
- Equidae
- Genes, Viral
- Genetic Variation
- Horse Diseases / virology
- Horses
- Molecular Sequence Data
- RNA, Viral / isolation & purification
- Sequence Alignment
- Sequence Homology
- Sheep
- Sheep Diseases / genetics
- Viral Proteins / genetics
Citations
This article has been cited 20 times.- Sunagawa J, Komorizono R, Park H, Hart WS, Thompson RN, Makino A, Tomonaga K, Iwami S, Yamaguchi R. Contact-number-driven virus evolution: A multi-level modeling framework for the evolution of acute or persistent RNA virus infection. PLoS Comput Biol 2023 May;19(5):e1011173.
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- Sauder C, Müller A, Cubitt B, Mayer J, Steinmetz J, Trabert W, Ziegler B, Wanke K, Mueller-Lantzsch N, de la Torre JC, Grässer FA. Detection of Borna disease virus (BDV) antibodies and BDV RNA in psychiatric patients: evidence for high sequence conservation of human blood-derived BDV RNA. J Virol 1996 Nov;70(11):7713-24.
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