FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Equine veterinary journal1989; 21(5); 325-331; doi: 10.1111/j.2042-3306.1989.tb02681.x

Sequential clinical and synovial fluid changes associated with acute infectious arthritis in the horse.

Abstract: Infectious arthritis was induced experimentally in one tarsocrural joint of six horses by intra-articular injection of 1 ml Staphylococcus-saline suspension containing 9 x 10(4) to 3 x 10(6) organisms. The corresponding contralateral joint was injected with 1 ml of saline and served as a control. The progression of the induced infectious arthritis was assessed over a nine-day period by clinical examination and sequential synovial fluid analysis with pH and lactate measurements. Changes in synovial fluid were present before clinical signs of infectious arthritis were manifested. The diagnostic value of different synovial fluid parameters at various stages of infection was determined. Cellular changes initially preceded the biochemical changes. Total leucocyte counts showed a significant increase within 24 h (up to 100 x 10(9)/litre) with great variability in subsequent measurements. Neutrophilia over 90 per cent and pH under 6.9 were the most consistent findings in the infected synovia. Increased total protein was also significant and was progressive throughout the experiment. Serum and synovial glucose difference and synovial lactate had more diagnostic value in the acute stages than in the chronic stages. The control joints elicited an inflammatory response manifested by increased leucocyte count, moderate neutrophilia, slightly increased total protein concentration, and slightly decreased pH, but all reactions were minor in comparison to those in the infected joints.
Get Full Text
Publication Date: 1989-09-01 PubMed ID: 2776718DOI: 10.1111/j.2042-3306.1989.tb02681.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the progression of infectious arthritis induced experimentally in horses. The results showed that changes in synovial fluid could be detected before clinical signs of the disease, with certain parameters more diagnostic in the early stages of the infection.

Methodology

  • Six horses were injected with a Staphylococcus-saline suspension to induce infectious arthritis in one of their tarsocrural joints.
  • The respective contralateral joint was injected with saline only and served as a control.
  • Scientists performed a sequence of clinical examinations and synovial fluid analyses over nine days, studying changes in pH and lactate levels.

Findings

  • Notably, changes in synovial fluid were evident before the clinical presentation of the disease.
  • The research team observed that cellular alterations appeared before biochemical changes.
  • Total leucocyte (white blood cell) counts increased significantly within 24 hours after the induction, while subsequent measurements witnessed high variability.
  • They found that neutrophilia over 90% and pH under 6.9 in the infected synovial fluid were the most consistent findings.
  • There was also a meaningful and progressive increase in the total protein over the time.

Diagnostic Value

  • The difference between serum and synovial glucose and synovial lactate levels were found to provide more diagnostic value in the acute stages of infection than in the chronic stages.
  • Meanwhile, the control joints also showed an inflammatory response, reflected by increased leucocyte count, moderate neutrophilia, slightly elevated total protein concentration, and slightly reduced pH.
  • Nonetheless, these reactions were minimal compared to the responses observed in infected joints.

Implications

  • The research contributes valuable information for the early diagnosis of infectious arthritis in horses, potentially allowing for more effective treatment.
  • The observed alterations in synovial fluid parameters could act as indicators for the onset of disease, even before clinical symptoms develop.
  • However, further studies are necessary to confirm these markers and to improve our understanding of the disease progression.

Cite This Article

APA
Tulamo RM, Bramlage LR, Gabel AA. (1989). Sequential clinical and synovial fluid changes associated with acute infectious arthritis in the horse. Equine Vet J, 21(5), 325-331. https://doi.org/10.1111/j.2042-3306.1989.tb02681.x

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 21
Issue: 5
Pages: 325-331

Researcher Affiliations

Tulamo, R M
  • College of Veterinary Medicine, Department of Clinical Sciences, Helsinki, Finland.
Bramlage, L R
    Gabel, A A

      MeSH Terms

      • Acute Disease
      • Animals
      • Arthritis, Infectious / complications
      • Arthritis, Infectious / diagnosis
      • Arthritis, Infectious / veterinary
      • Blood Glucose / analysis
      • Glucose / analysis
      • Horse Diseases / diagnosis
      • Horse Diseases / etiology
      • Horses
      • Hydrogen-Ion Concentration
      • Lactates / analysis
      • Lameness, Animal / etiology
      • Leukocyte Count / veterinary
      • Neutrophils
      • Proteins / analysis
      • Staphylococcal Infections / complications
      • Staphylococcal Infections / diagnosis
      • Staphylococcal Infections / veterinary
      • Synovial Fluid / analysis
      • Synovial Fluid / cytology
      • Synovial Fluid / microbiology

      Citations

      This article has been cited 11 times.
      1. Stein C. Effects of pH on opioid receptor activation and implications for drug design. Biophys J 2024 Dec 17;123(24):4158-4166.
        doi: 10.1016/j.bpj.2024.07.007pubmed: 38970252google scholar: lookup
      2. Kearney CM, Korthagen NM, Plomp SGM, Labberté MC, de Grauw JC, van Weeren PR, Brama PAJ. A Translational Model for Repeated Episodes of Joint Inflammation: Welfare, Clinical and Synovial Fluid Biomarker Assessment. Animals (Basel) 2023 Oct 12;13(20).
        doi: 10.3390/ani13203190pubmed: 37893914google scholar: lookup
      3. Kearney CM, Khatab S, van Buul GM, Plomp SGM, Korthagen NM, Labberté MC, Goodrich LR, Kisiday JD, Van Weeren PR, van Osch GJVM, Brama PAJ. Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation. Front Vet Sci 2022;9:907616.
        doi: 10.3389/fvets.2022.907616pubmed: 35812845google scholar: lookup
      4. Avellar HK, Lutter JD, Ganta CK, Beard W, Smith JR, Jonnalagadda N, Peloquin S, Kang Q, Ayub K. In vitro antimicrobial activity of equine platelet lysate and mesenchymal stromal cells against common clinical pathogens. Can J Vet Res 2022 Jan;86(1):59-64.
        pubmed: 34975224
      5. Haralambus R, Florczyk A, Sigl E, Gültekin S, Vogl C, Brandt S, Schnierer M, Gamerith C, Jenner F. Detection of synovial sepsis in horses using enzymes as biomarkers. Equine Vet J 2022 May;54(3):513-522.
        doi: 10.1111/evj.13459pubmed: 33977535google scholar: lookup
      6. Klein R, Nagy O, Tóthová C, Chovanová F. Clinical and Diagnostic Significance of Lactate Dehydrogenase and Its Isoenzymes in Animals. Vet Med Int 2020;2020:5346483.
        doi: 10.1155/2020/5346483pubmed: 32607139google scholar: lookup
      7. Stack JD, Cousty M, Steele E, Handel I, Lechartier A, Vinardell T, David F. Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment. Front Vet Sci 2019;6:325.
        doi: 10.3389/fvets.2019.00325pubmed: 31632987google scholar: lookup
      8. Niemelä TM, Tulamo RM, Carmona JU, López C. Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses. Acta Vet Scand 2019 May 30;61(1):24.
        doi: 10.1186/s13028-019-0460-6pubmed: 31146775google scholar: lookup
      9. Koziy RV, Yoshimura S, Dickinson R, Rybicka JM, Moshynskyy I, Ngeleka M, Bracamonte JL, Simko E. Use of standard diagnostic techniques to determine eradication of infection in experimental equine septic arthritis. Can J Vet Res 2019 Jan;83(1):24-33.
        pubmed: 30670899
      10. Moreira JJ, Moraes AP, Brossi PM, Machado TS, Michelacci YM, Massoco CO, Baccarin RY. Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints. J Vet Sci 2015;16(1):47-55.
        doi: 10.4142/jvs.2015.16.1.47pubmed: 25269714google scholar: lookup
      11. MacWilliams PS, Friedrichs KR. Laboratory evaluation and interpretation of synovial fluid. Vet Clin North Am Small Anim Pract 2003 Jan;33(1):153-78.
        doi: 10.1016/s0195-5616(02)00083-9pubmed: 12512381google scholar: lookup
      Subscribe to our newsletter
      Join 99,970 horse owners like you who receive our email updates on horse health and nutrition.