Sequential concentrations of placental growth factor and haptoglobin, and their relation to oestrone sulphate and progesterone in pregnant Spanish Purebred mare.
Abstract: The objectives of this study were to establish reference values for serum concentrations of placental growth factor (PlGF) and haptoglobin (Hp), and to analyze whether the levels of oestrone sulphate (E1S) and progesterone (P4) are physiologically involved in the dynamic modifications of the above parameters in pregnant mares. A total of 30 healthy Spanish Purebred mares ranging in age 9.33 ± 3.31 years were studied during the 11 months of gestation. Serum concentrations of PlGF were detected by EIA, Hp using commercial Phase Haptoglobin assay and E1S and P4 levels through RIA. The serum concentrations of PlGF ranged between 31.70 and 223.60 ng/mL, with a mean value of 57.64 ± 18.05 ng/mL. Serum PlGF levels increased significantly during the 1st and 2nd months, reaching the maximum value in the 3rd month and the minimum value in the 10th month. Hp concentrations increased progressively and significantly from the 5th until the 10th month of gestation (P < 0.05), decreasing in the 11th month of pregnancy. E1S increased significantly from the 3rd until the 7th month, decreasing progressively towards the end of gestation. P4 increased significantly in the 3rd and 4th month and decreased significantly in the 6th and 7th (P < 0.05), with variable oscillations during last months of pregnancy. PlGF and Hp were significantly and negatively correlated (r = -0.27; P < 0.05). In the healthy mare, PlGF and Hp act asynchronously and independent of steroid E1S and P4.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication Date: 2018-04-27 PubMed ID: 29730538DOI: 10.1016/j.theriogenology.2018.04.033Google Scholar: Lookup
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Summary
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The research investigates the levels of two particular proteins, placental growth factor (PlGF) and haptoglobin (Hp), in pregnant Spanish Purebred mares, and explores their relations with hormones oestrone sulphate (ES) and progesterone (P) throughout the length of pregnancy.
About the Study
- The study aims to determine reference values for serum concentrations of PlGF and Hp, and examine their relationship with steroid hormones, ES and P, during the gestation period in healthy Spanish Purebred mares.
- A sample size of 30 healthy mares were studied over the course of their 11-month gestation period.
- The concentrations of PlGF and Hp in the serum were detected using various assays while the levels of ES and P were established through Radioimmunoassay (RIA).
Findings and Results
- They established that PlGF serum concentrations ranged between 31.70 and 223.60 ng/mL, with an average value of 57.64±18.05 ng/mL.
- It observed that serum PlGF levels noticeably elevated during the initial two months of pregnancy, reaching the peak in the third month and the lowest level in the tenth month.
- Hp concentrations showed a significant and progressive increase from the fifth month until the tenth month of pregnancy, decreasing in the last month of pregnancy.
- ES levels rose significantly from the third month up until the seventh month, but then slowly decreased towards the end of gestation.
- Progesterone levels significantly increased in the third and fourth months and fell significantly again in the sixth and seventh months, fluctuating variably towards the last stages of pregnancy.
Conclusion and Further Analysis
- It was detected that PlGF and Hp had a significant and negative correlation.
- The findings suggest that in healthy mares, the factors PlGF and Hp behave asynchronously and operate independently of the hormones ES and P.
- The study presents a valuable contribution to our understanding of physiological changes and their interactions during equine pregnancy, as well as providing a reference for further research into reproductive biology in horses.
Cite This Article
APA
Satué K, Marcilla M, Medica P, Ferlazzo A, Fazio E.
(2018).
Sequential concentrations of placental growth factor and haptoglobin, and their relation to oestrone sulphate and progesterone in pregnant Spanish Purebred mare.
Theriogenology, 115, 77-83.
https://doi.org/10.1016/j.theriogenology.2018.04.033 Publication
Researcher Affiliations
- Department of Animal Medicine and Surgery, Faculty of Veterinary, Cardenal Herrera-CEU University, Valencia, Spain. Electronic address: ksatue@uchceu.es.
- Department of Animal Medicine and Surgery, Faculty of Veterinary, Cardenal Herrera-CEU University, Valencia, Spain.
- Department of Veterinary Sciences, Unit Veterinary Physiology, Polo Universitario Annunziata, Messina University, Italy.
- Department of Veterinary Sciences, Unit Veterinary Physiology, Polo Universitario Annunziata, Messina University, Italy.
- Department of Veterinary Sciences, Unit Veterinary Physiology, Polo Universitario Annunziata, Messina University, Italy.
MeSH Terms
- Animals
- Estrone / analogs & derivatives
- Estrone / blood
- Female
- Gestational Age
- Haptoglobins / analysis
- Horses / blood
- Placenta Growth Factor / blood
- Pregnancy
- Pregnancy, Animal / blood
- Progesterone / blood
- Reference Values
Citations
This article has been cited 5 times.- Scoggin KE, Adlan F, Fedorka CE, Rakha SI, Stout TAE, Troedsson MHT, Ali HE. Gestation-Stage Related Changes in the IGF System Components in the Equine Placenta. Biomolecules 2025 Aug 6;15(8).
- Ozturan HG, Aslan S, Zabitler Tepik F, Darbaz I, Sayiner S, Wehrend A. Concentrations of Progesterone (P(4)), Anti-Müllerian Hormone (AMH), and Haptoglobin (Hp) in Pregnant and Non-Pregnant Ewes and Their Association with Fetal Mortality, Maternal Weight, and Twinning Rate. Vet Sci 2025 May 12;12(5).
- Veronesi MC, Cotticelli A, Pividori I, Giombolini M, Corazzin M, Ellero L, Peric T. From Pre-Foaling to Late Pregnancy: Cortisol, DHEA(S), Progesterone, 17-β-Estradiol, and Allopregnanolone Hair Concentration Profiles in Standardbred Mares. Animals (Basel) 2025 Jan 23;15(3).
- Satué K, Fazio E, La Fauci D, Medica P. Changes of Hepcidin, Ferritin and Iron Levels in Cycling Purebred Spanish Mares. Animals (Basel) 2023 Mar 31;13(7).
- Satué K, Calvo A, Muñoz A, Fazio E, Medica P. Interrelationship between reproductive hormones and acute phase proteins during estrous cycle and pregnancy in Spanish purebred broodmares. Vet Anim Sci 2021 Dec;14:100212.
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