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BMC veterinary research2020; 16(1); 422; doi: 10.1186/s12917-020-02629-4

Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

Abstract: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 h after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). Results: Compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 h post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day. Conclusions: The results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.
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Publication Date: 2020-11-04 PubMed ID: 33148245PubMed Central: PMC7641807DOI: 10.1186/s12917-020-02629-4Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the changes in activity of Paraoxonase-1 (PON-1), an antioxidant enzyme, in horses during endotoxemia induced experimentally. The results indicated a decrease in enzyme activity post-infusion which normalizes on the tenth day, suggesting the potential use of PON-1 activity measurements in monitoring the progression and recovery from endotoxemia in horses.

Objective and Methodology

  • The research aimed to study the behavior of the antioxidant enzyme Paraoxonase-1 (PON-1) in horses subjected to induced endotoxemia. Previous studies had highlighted the potential value of PON-1 in diagnosing Systemic Inflammatory Response Syndrome (SIRS) and predicting outcomes in diseased horses. The research aimed to provide more insights into this possibility.
  • To understand PON-1 behaviour, it’s activity was measured in plasma samples obtained from six healthy mares. These samples were collected at multiple points in timeline, namely before and between 12 and 240 hours post intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS).

Results

  • After the infusion of LPS, a progressive yet transient decrease in PON-1 activity was observed starting from 24 hours post-infusion. This decreasing trend sank to the lowest level between 3 to 7 days after infusion.
  • Interestingly, this low enzyme activity bounced back to normalized pre-infusion levels on the tenth day after LPS infusion.

Conclusions and Implications

  • This study provided preliminary evidence that monitoring PON-1 activity might help assess progression and recovery from endotoxemia in horses. The transient decrease in activity followed by normalization could serve as a potential biomarker for Study Investigates Paraoxonase-1 (PON-1) Activity in Horses.
  • The authors recommended further studies on horses with naturally occurring sepsis to solidify the potential diagnostic value of PON-1 activity measurements.

Cite This Article

APA
Scavone D, Sgorbini M, Borges AS, Oliveira-Filho JP, Vitale V, Paltrinieri S. (2020). Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia. BMC Vet Res, 16(1), 422. https://doi.org/10.1186/s12917-020-02629-4

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 422
PII: 422

Researcher Affiliations

Scavone, Donatella
  • Department of Veterinary Medicine, Veterinary Teaching Hospital, University of Milan, Lodi, Italy.
Sgorbini, Micaela
  • Department of Veterinary Sciences, Veterinary Teaching Hospital, Univeristy of Pisa, Pisa, Italy. micaela.sgorbini@unipi.it.
Borges, Alexandre S
  • Department of Veterinary Clinical Science, School of Veterynary Medicine and Animal Sicence, Sao PAulo State University (Unesp), Botucatu, Brazil.
Oliveira-Filho, José P
  • Department of Veterinary Clinical Science, School of Veterynary Medicine and Animal Sicence, Sao PAulo State University (Unesp), Botucatu, Brazil.
Vitale, Valentina
  • School of Veterinary Science, Veterinary Teaching Hospital, University of Sydney, Camden, NSW, Australia.
Paltrinieri, Saverio
  • Department of Veterinary Medicine, Veterinary Teaching Hospital, University of Milan, Lodi, Italy.

MeSH Terms

  • Animals
  • Aryldialkylphosphatase / blood
  • Endotoxemia / blood
  • Endotoxemia / chemically induced
  • Endotoxemia / diagnosis
  • Endotoxemia / enzymology
  • Escherichia coli
  • Female
  • Horse Diseases / blood
  • Horse Diseases / diagnosis
  • Horse Diseases / enzymology
  • Horses
  • Lipopolysaccharides / administration & dosage

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 3 times.
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