Serodiagnosis of equine infectious anemia by agar gel immunodiffusion and ELISA using a recombinant p26 viral protein expressed in Escherichia coli as antigen.
Abstract: We used a p26 recombinant protein (p26r) from equine infectious-anemia virus (EIAV) expressed in Escherichia coli as antigen to standardize an agar-gel immunodiffusion (AGIDp26r) test and an indirect ELISA (ELISAp26r) for the detection of antibodies against EIAV in 720 equine sera from Brazil. We evaluated the tests's relative diagnostic sensitivities (relSe) and relative diagnostic specificities (relSp) against a commercial AGID kit (Idexx, USA). We used three sera panels: panel A--196 AGID-negative sera from an AIE non-endemic controlled area; panel B--194 AGID-negative sera from an AIE endemic area and panel C--330 AGID-positive sera from an AIE endemic area. ELISAp26r cut-off value was defined with TG-ROC using sera from panels A and C. AGIDp26r showed an agreement of 100% with the commercial kit. When applied to sera from panels A and C, ELISAp26r showed an agreement of 100% with the kit, but, although relSe was 100% for panel C, the ELISAp26r had relSp of 93.3%.
Publication Date: 2006-11-15 PubMed ID: 17109980DOI: 10.1016/j.prevetmed.2006.10.009Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article is about using a p26 protein and E.coli to standardize tests to detect antibodies against Equine Infectious Anemia Virus in horse serum from Brazil. The results were compared to a commercial kit. The study found that both tests showed a high level of agreement with the commercial kit.
Research Context
- This study was conducted to evaluate the effectiveness of using a p26 protein from the Equine Infectious Anemia Virus (EIAV) expressed in Escherichia coli as an antigen.
- The antigen was used to standardize two tests: an Agar-Gel Immunodiffusion (AGIDp26r) test and an indirect Enzyme-Linked Immunosorbent Assay (ELISAp26r).
- The tests were used to detect antibodies against EIAV in the serum of 720 horses from Brazil.
Study Design and Methodology
- Three panels of serum were used in the study: Panel A which included 196 AGID-negative serum from a non-endemic area for EIAV; Panel B which included 194 AGID-negative serum from an endemic area for EIAV; and Panel C which included 330 AGID-positive serum from an endemic area for EIAV.
- The relative diagnostic sensitivities (relSe) and relative diagnostic specificities (relSp) of the two tests were evaluated against a commercial AGID kit by Idexx, USA.
- The cut-off value for ELISAp26r was defined using TG-ROC and serum from Panels A and C.
Research Findings
- The AGIDp26r test showed a 100% agreement with the commercial Idexx kit.
- The ELISAp26r test showed a 100% agreement with the Idexx kit when it was applied to serum from Panels A and C.
- For Panel C serum, the ELISAp26r demonstrated a 100% relSe.
- However, the ELISAp26r only had a relSp of 93.3% indicating that there were some false positive results.
Cite This Article
APA
Piza AS, Pereira AR, Terreran MT, Mozzer O, Tanuri A, Brandão PE, Richtzenhain LJ.
(2006).
Serodiagnosis of equine infectious anemia by agar gel immunodiffusion and ELISA using a recombinant p26 viral protein expressed in Escherichia coli as antigen.
Prev Vet Med, 78(3-4), 239-245.
https://doi.org/10.1016/j.prevetmed.2006.10.009 Publication
Researcher Affiliations
- Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, CEP 05508-270, São Paulo, SP, Brazil.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Antigens, Viral / immunology
- Brazil / epidemiology
- Enzyme-Linked Immunosorbent Assay / methods
- Enzyme-Linked Immunosorbent Assay / standards
- Enzyme-Linked Immunosorbent Assay / veterinary
- Equine Infectious Anemia / diagnosis
- Equine Infectious Anemia / epidemiology
- Escherichia coli / virology
- Horses
- Immunodiffusion / methods
- Immunodiffusion / standards
- Immunodiffusion / veterinary
- Reagent Kits, Diagnostic / standards
- Reagent Kits, Diagnostic / veterinary
- Sensitivity and Specificity
- Seroepidemiologic Studies
- Time Factors
- Viral Core Proteins / immunology
Citations
This article has been cited 5 times.- Hu Z, Guo K, Du C, Sun J, Naletoski I, Chu X, Lin Y, Wang X, Barrandeguy M, Samuel M, Wang W, Lau PI, Wernery U, Raghavan R, Wang X. Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia. Appl Microbiol Biotechnol 2023 May;107(10):3305-3317.
- Alnaeem AA, Hemida MG. Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016. Vet World 2019 May;12(5):719-723.
- Cruz F, Fores P, Ireland J, Moreno MA, Newton R. Freedom from equine infectious anaemia virus infection in Spanish Purebred horses. Vet Rec Open 2015;2(1):e000074.
- Singha H, Goyal SK, Malik P, Khurana SK, Singh RK. Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus. Indian J Virol 2013 Dec;24(3):349-56.
- Wang J, Qiu J, Wang M, Wu X, Li X, Zhang H. Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus. Virol J 2025 Jun 24;22(1):205.
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