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Journal of clinical microbiology1997; 35(1); 169-173; doi: 10.1128/jcm.35.1.169-173.1997

Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays.

Abstract: Serum samples from dogs and equids suspected of having canine or equine borreliosis, respectively, were analyzed in polyvalent enzyme-linked immunosorbent assays (ELISAs) with whole-cell or recombinant antigens of Borrelia burgdorferi sensu stricto. Purified preparations of recombinant antigens included outer surface protein A (OspA), OspB, OspC, OspE, OspF, and p41-G (a fragment of flagellin). Of the 36 dog sera that reacted positively to whole-cell antigen, 32 (88.9%) contained antibodies to one or more recombinant antigens. Reactivities to OspF (88.9% positive) and p41-G (75% positive) were most prevalent. In analyses of 30 equid sera positive in an ELISA with whole cells, 24 (80%) contained antibodies to one or more recombinant antigens. Seropositivities in ELISAs with p41-G (50% positive) and OspF (46.7% positive) were more than twofold greater than in ELISAs with OspA, OspB, or OspC (10 to 20% positive). In parallel tests of eight canine and three equine sera, there was good agreement in results of Western blot (immunoblot) analyses and ELISAs. Although dog and equid sera with antibodies to whole-cell B. burgdorferi frequently reacted positively to one or more recombinant antigens, the inclusion of OspF and p41-G antigens in ELISAs was most useful in the serologic diagnosis of canine and equine borreliosis.
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Publication Date: 1997-01-01 PubMed ID: 8968901PubMed Central: PMC229532DOI: 10.1128/jcm.35.1.169-173.1997Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the use of recombinant antigens in the diagnosis of canine and equine borreliosis through enzyme-linked immunosorbent assays (ELISAs). The research found that the majority of serum samples from dogs and equids suspected of borreliosis contained antibodies reacting positively to the recombinant antigens, particularly outer surface protein A, OspF and p41-G, suggesting their potential usefulness in diagnosing the disease.

Analysing Serum Samples

  • This study began by collecting serum samples from dogs and equids (horses, ponies, etc.) that were suspected of having canine or equine borreliosis, respectively. These are conditions caused by a type of bacteria called Borrelia burgdorferi.
  • The researchers then analyzed these samples using enzyme-linked immunosorbent assays (ELISAs) — widely-used tests that detect and measure antibodies in the blood.
  • The ELISAs in question here were polyvalent, meaning they could identify antibodies against more than one antigen. They used either whole-cell or recombinant antigens of B. burgdorferi.

Recombinant Antigens

  • Recombinant antigens used in the ELISAs included purified preparations of proteins from the outer surface of B. burgdorferi bacteria (OspA, OspB, OspC, OspE, OspF), and a fragment of a protein called flagellin (p41-G).
  • The antigens are “recombinant” because they’re produced using genetic engineering techniques, allowing scientists to create pure samples of specific proteins for use in tests.

Diagnostic Efficacy

  • The majority of the dog and equine sera tested contained antibodies against at least one of these antigens, indicating exposure to B. burgdorferi.
  • The most common antibodies identified in the samples were against OspF and p41-G.
  • These results were also confirmed using Western blot (another method to detect specific proteins in a blood sample).

Conclusion

  • The study suggested that for the serologic diagnosis of canine and equine borreliosis, the inclusion of OspF and p41-G antigens in ELISAs is particularly useful.
  • This research helps to refine and inform diagnostic techniques for borreliosis – a condition that is a significant health concern for both animals and humans.

Cite This Article

APA
Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. (1997). Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. J Clin Microbiol, 35(1), 169-173. https://doi.org/10.1128/jcm.35.1.169-173.1997

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 35
Issue: 1
Pages: 169-173

Researcher Affiliations

Magnarelli, L A
  • Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504, USA.
Flavell, R A
    Padula, S J
      Anderson, J F
        Fikrig, E

          MeSH Terms

          • Animals
          • Antigens, Bacterial / immunology
          • Borrelia burgdorferi Group / immunology
          • Borrelia burgdorferi Group / isolation & purification
          • Dog Diseases / diagnosis
          • Dogs
          • Enzyme-Linked Immunosorbent Assay / methods
          • Equidae
          • Lyme Disease / diagnosis
          • Lyme Disease / veterinary
          • Recombinant Proteins / immunology
          • Serologic Tests

          Grant Funding

          • AI-49988 / NIAID NIH HHS
          • CCU-106581 / PHS HHS
          • P0-1-AI-30548 / NIAID NIH HHS

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          Citations

          This article has been cited 12 times.
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