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Archives of Razi Institute2022; 77(3); 1105-1111; doi: 10.22092/ARI.2022.357270.2009

Serological and Histopathological Investigation of Chlamydia abortus in Aborted Ewes in Wasit, Iraq.

Abstract: is one of the most important pathogens, which causes a marked economic loss in small ruminants, in particular sheep, worldwide. This study aimed to detect the prevalence of in the sera of aborted ewes in Wasit province, Iraq, using the enzyme-linked immunosorbent assay (ELISA), followed by the investigation of the main histopathological alterations that occurred in some organs of the dead newborns. Out of 180 tested samples by ELISA, 32.22% of the evaluated animals showed positive reactions to IgG antibodies toward . Concerning the titers of the infection of seropositive ewes, there were significant increases in values of moderate level of antibody titer (55.17%), compared to mild (32.76%) and severe (12.07%) levels of infection. History data showed a significant variation (<0.05) in the existence of seropositive ewes with other field animals. However, significant increases (<0.05) were reported in the seropositive ewes existed with goat (91.38%) and cattle (84.48%), while significant decreases (<0.05) were observed in positive ewes found in donkeys (8.62%), horses (3.45%), and camels (0%). Regarding the histopathology results, the findings of the small intestine showed marked necrosis of intestinal villous, hyperplastic tissues, and necrosis in some mucosal glands; however, in the liver, there was small ductal proliferation with mild portal fibrosis, necrotic debris, and focal areas of hemorrhage in the parenchyma. In conclusion, the findings of this study represent the first Iraqi data concerning detection in aborted ewes; however, further studies in other regions and animal species are necessary to know the actual prevalence of organisms and initiate active measures for control and prevention.
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Publication Date: 2022-06-30 PubMed ID: 36618285PubMed Central: PMC9759238DOI: 10.22092/ARI.2022.357270.2009Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on identifying the prevalence of Chlamydia abortus, a significant cause of economic loss in small ruminants like sheep, in Wasit province, Iraq. Using antibody tests and examination of newborn organ damage, researchers found that approximately one in three tested sheep had been exposed to the bacterium, with goats and cattle frequently cohabitating with the affected sheep.

Antibody Testing and Histopathology

  • The researchers used an enzyme-linked immunosorbent assay (ELISA), a test that detects and measures antibodies in the blood, to identify exposure to Chlamydia abortus in the blood serum of sheep who had spontaneously aborted their lambs.
  • Out of 180 tested samples, 32.22% demonstrated positive antibody reactions to Chlamydia abortus, indicating infection or past exposure.
  • Antibody levels varied in the infected sheep, with 55.17% displaying a moderate level of antibodies, 32.76% displaying a mild reaction, and 12.07% indicating severe infection.
  • The researchers also conducted a histopathology investigation, observing organ and tissue damage in the stillborn lambs caused by the bacterial infection which showed marked effects on the liver and small intestines.

Cohabitation Impacts

  • In examining the living conditions of the infected ewes, the study uncovered significant variations in the presence of Chlamydia abortus in sheep cohabiting with other types of livestock.
  • High prevalence was found in farms where sheep lived with goats (91.38%) and cattle (84.48%).
  • In contrast, lower prevalence was found when sheep were cohabitating with donkeys (8.62%), horses (3.45%), and camels (0%).

Significance of the Research

  • This study provides the first data on Chlamydia abortus prevalence in aborted ewes in Iraq.
  • The researchers conclude that further study in other regions and animal species is necessary to understand the true prevalence of the pathogen and develop effective control and prevention measures.

Cite This Article

APA
Ali HHM, Al-Bayati LH. (2022). Serological and Histopathological Investigation of Chlamydia abortus in Aborted Ewes in Wasit, Iraq. Arch Razi Inst, 77(3), 1105-1111. https://doi.org/10.22092/ARI.2022.357270.2009

Publication

Archives of Razi Institute
ISSN: 2008-9872
NlmUniqueID: 101549567
Country: Iran
Language: English
Volume: 77
Issue: 3
Pages: 1105-1111

Researcher Affiliations

Ali, H H M
  • Department of Pathology, College of Medicine, Wasit University, Wasit, Iraq.
Al-Bayati, L H
  • Department of Microbiology, College of Medicine, Wasit University, Wasit, Iraq.

MeSH Terms

  • Pregnancy
  • Cattle
  • Sheep
  • Animals
  • Female
  • Horses
  • Iraq / epidemiology
  • Sheep Diseases / epidemiology
  • Abortion, Veterinary / epidemiology
  • Goats
  • Cattle Diseases
  • Goat Diseases
  • Horse Diseases

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Citations

This article has been cited 1 times.
  1. Nogarol C, Marchino M, Scala S, Belvedere M, Renna G, Vitale N, Mandola ML. Seroprevalence and Risk Factors Associated with Chlamydia abortus Infection in Sheep and Goats in North-Western Italy. Animals (Basel) 2024 Jan 17;14(2).
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