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Home / Equine Research Bank / Equine Vet J / Serological detection of equid herpesvirus 1 infections of t...
Equine veterinary journal1976; 8(2); 58-65; doi: 10.1111/j.2042-3306.1976.tb03291.x

Serological detection of equid herpesvirus 1 infections of the respiratory tract.

Abstract: An investigation was made of 3 serological tests (virus neutralization, complement fixation and indirect immunofluorescence), which are applicable to epidemiological studies of infections by Equid herpesvirus 1 (EHV-1). Sera from gnotobiotic foals inoculated intranasally with various strains of EHV-1 were unable in some cases to neutralize heterologous strains and these results were not consistent with the existence of clearly-defined subtypes of EHV-1, as previously proposed. The cross-reactions in complement-fixation tests paralleled those with neutralization but immunofluorescence tests were found to be both more sensitive and more broadly reactive than the other two. Complement-fixing antibodies declined more rapidly following experimental infection than did those measured by neutralization or immunofluorescence. The results are discussed in relation to the diagnosis of EHV-1 infection and the significance they may have for the epidemiology of this disease.
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Publication Date: 1976-04-01 PubMed ID: 177281DOI: 10.1111/j.2042-3306.1976.tb03291.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the comparison of three serological tests, namely virus neutralization, complement fixation, and indirect immunofluorescence, to study Equid herpesvirus 1 (EHV-1) infections in horses. The goal was to evaluate their sensitivity and efficiency in detecting the virus, with implications for diagnosing EHV-1 and understanding its epidemiology.

Introduction and Methodology

  • This research undertakes an investigation of three serological tests – virus neutralization, complement fixation, and indirect immunofluorescence – to study the epidemiology of EHV-1 infections.
  • Serological tests are diagnostic methods used to identify antibodies in the serum, providing insight into an individual’s exposure to a particular infectious agent, in this case, EHV-1.
  • The researchers used sera (the clear liquid part of the blood) from gnotobiotic foals (foals in a completely sterile environment) that were intranasally inoculated with different strains of EHV-1.

Findings and Results

  • The sera from foals could not neutralize certain heterologous strains. This outcome aligns inconsistently with the existence of distinctly defined EHV-1 subtypes.
  • These results show that cross-reactions in the complement-fixation test were similar to those found in neutralization. However, the indirect immunofluorescence tests proved more sensitive and reactive than the other two tests.
  • It was also found that complement-fixing antibodies declined more rapidly after the infection than those detected by neutralization or immunofluorescence.
  • This suggests that while the indirect immunofluorescence test may be more broadly applicable and more sensitive to EHV-1, the complement-fixation test provides higher specificity for detecting and tracking the stage of EHV-1 infection.

Implications and Conclusion

  • The study sheds important light on the diagnosis methods of EHV-1 infection, which can influence the treatment and management of outbreaks in equine populations.
  • The results’ implications may impact understanding of the epidemiology of EHV-1 – detailing how the virus spreads among horses, its prevalence, and how it might be halted.

Cite This Article

APA
Thomson GR, Mumford JA, Campbell J, Griffiths L, Clapham P. (1976). Serological detection of equid herpesvirus 1 infections of the respiratory tract. Equine Vet J, 8(2), 58-65. https://doi.org/10.1111/j.2042-3306.1976.tb03291.x

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 8
Issue: 2
Pages: 58-65

Researcher Affiliations

Thomson, G R
    Mumford, J A
      Campbell, J
        Griffiths, L
          Clapham, P

            MeSH Terms

            • Animals
            • Complement Fixation Tests
            • Cross Reactions
            • Fluorescent Antibody Technique
            • Herpesviridae
            • Herpesviridae Infections / diagnosis
            • Herpesviridae Infections / immunology
            • Herpesviridae Infections / veterinary
            • Herpesvirus 1, Equid / immunology
            • Horse Diseases / diagnosis
            • Horse Diseases / immunology
            • Horses
            • Neutralization Tests
            • Respiratory Tract Infections / diagnosis
            • Respiratory Tract Infections / immunology
            • Respiratory Tract Infections / veterinary

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