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Home / Equine Research Bank / J Vet Res / Seroprevalence of Antibodies Against Anaplasma Phagocytophil...
Journal of veterinary research2020; 64(3); 413-419; doi: 10.2478/jvetres-2020-0045

Seroprevalence of Antibodies Against Anaplasma Phagocytophilum and Borrelia Burgdorferi in Horses (Equus Caballus) from Northern Algeria.

Abstract: Horses (Equus caballus) are susceptible to tick-borne diseases. Two of them, Lyme borreliosis due to Borrelia burgdorferi and granulocytic anaplasmosis due to Anaplasma phagocytophilum were investigated in Algerian horses. The diseases have been less extensively studied in horses and results pertinent to Algeria have not been published. Methods: Blood samples were obtained from 128 horses. IgG antibodies directed against Anaplasma phagocytophilum and Borrelia burgdorferi were detected by an indirect immunofluorescence antibody test (IFAT) and ELISA. The potential effects of age, gender, breed, and health status on seropositivity were also evaluated. Results: Using IFAT, 28 (21.8%) and 25 (19.5%) animals were positive for B. burgdorferi and A. phagocytophilum, respectively. Using ELISA, 19 (14.8%) and 33 (25.9%) animals were positive for these bacteria. Conclusions: The study shows that horses in Algeria are exposed or co-exposed to tick-transmitted zoonotic bacterial species.
© 2020 A. Laamari et al. published by Sciendo.
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Publication Date: 2020-07-14 PubMed ID: 32984632PubMed Central: PMC7497754DOI: 10.2478/jvetres-2020-0045Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study involves researching the presence of diseases in horses in North Algeria, specifically those caused by the parasites Anaplasma phagocytophilum and Borrelia burgdorferi, which are predominantly transmitted by ticks. The study found a significant number of horses had been exposed to these parasites, indicating a possible health risk for the animals.

Objectives and Methodology

The primary aim of this epidemiological study was to monitor the seroprevalence, or the level of a pathogen in a population as measured in blood serum, of two specific diseases in horses in North Algeria. The diseases in question are Lyme borreliosis, caused by the Borrelia burgdorferi bacterium and granulocytic anaplasmosis, attributable to the Anaplasma phagocytophilum bacterium.

  • The research team took blood samples from 128 horses for this study.
  • The methods utilized to identify the presence of the relevant antibodies against the bacteria included the indirect immunofluorescence antibody test (IFAT) and the ELISA test, both widely used in seroprevalence studies.
  • The team also evaluated whether extrinsic factors such as age, breed, gender, and overall health status could influence seropositivity, the condition of having a detectable level of antibodies in the blood serum.

Results and Conclusions

The results established noteworthy exposure among horses to the above-mentioned infections, suggesting vulnerability to tick-borne diseases.

  • Based on IFAT, Borrelia burgdorferi was found in 21.8% of the horses sampled while Anaplasma phagocytophilum affected 19.5%.
  • ELISA test results indicated 14.8% horses were affected by Borrelia burgdorferi and 25.9% by Anaplasma phagocytophilum.
  • The study indicates that horses in Algeria are at risk of exposure or co-exposure to tick-transmitted bacterial species, with important health implications for these animals. This prevalence also suggests possible zoonotic transmission, as these diseases could potentially spread to humans through tick bites.

Cite This Article

APA
Laamari A, Azzag N, Tennah S, Derdour SY, China B, Boꯚllah R, Ghalmi F. (2020). Seroprevalence of Antibodies Against Anaplasma Phagocytophilum and Borrelia Burgdorferi in Horses (Equus Caballus) from Northern Algeria. J Vet Res, 64(3), 413-419. https://doi.org/10.2478/jvetres-2020-0045

Publication

Journal of veterinary research
ISSN: 2450-7393
NlmUniqueID: 101696630
Country: Poland
Language: English
Volume: 64
Issue: 3
Pages: 413-419

Researcher Affiliations

Laamari, Abdelouahab
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.
Azzag, Naouelle
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.
Tennah, Safia
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.
Derdour, Salima-Yamina
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.
China, Bernard
  • Sciensano, Quality of Laboratories, B-1050 Brussels, Belgium.
Boꯚllah, Ryhan
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.
Ghalmi, Farida
  • Research Laboratory Management of Local Animal Resources, Higher National Veterinary School, El Alia, Oued Smar, 1615 Algiers, Algeria.

Conflict of Interest Statement

Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

References

This article includes 40 references
  1. Amusategui I, Sainz A, Tesouro M A. Serological evaluation of Anaplasma phagocytophilum infection in livestock in northwestern Spain.. Ann N Y Acad Sci 2006;1078:487–490.
    pubmed: 17114760
  2. Azzag N, Petit E, Gandoin C, Bouillin C, Ghalmi F, Haddad N, Boulouis H J. Prevalence of select vector-borne pathogens in stray and client-owned dogs from Algiers.. Comp Immunol Microbiol Infect Dis 2015;38:1–7.
    pubmed: 25638478
  3. Bensaid M, Belkahia H, Sayahi L, Aloui M, Jemli M H, Hadj Mohamed B, Sassi L, Darghouth M A, Djaïem A A, Bayoudh M, Messadi L. Première étude sérologique de la prévalence d’Anaplasma phagocytophilum chez le dromadaire Camelus dromedarius en Tunisie.. Bull Soc Pathol Exot 2014;107:1–6.
    pubmed: 24363017
  4. Bermann F, Davoust B, Fournier P E, Brisou-Lapointe A V, Brouqui P. Ehrlichia equi Anaplasma phagocytophila infection in an adult horse in France.. Vet Rec 2002;150:787–788.
    pubmed: 12135075
  5. Bhide M, Yilmaz Z, Golcu E, Torun S, Mikula I. Seroprevalence of anti-Borrelia burgdorferi antibodies in dogs and horses in Turkey.. Ann Agric Environ Med 2008;15:85–90.
    pubmed: 18581984
  6. Burgess B. British Columbia. Lyme disease in horses.. Can Vet J 1988;29:393–394.
    pmc: PMC1680893pubmed: 17423037
  7. Büscher G, Gandras R, Apel G, Friedhoff K T. The 1st case of ehrlichiosis in a horse in Germany.. Dtsch Tierarztl Wochenschr 1984;91:408–409.
    pubmed: 6397345
  8. Devilbiss B, Mohammed H O, Divers T J. Perception of equine practitioners regarding the occurrence of selected equine neurologic diseases in the Northeast over a 10-year period.. J Equine Vet Sci 2009;29:237–246.
  9. Durrani A Z, Goyal S M, Kamal N. Retrospective study on seroprevalence of Borrelia burgdorferi antibodies in horses in Minnesota.. T Equine Vet Sci 2011;31:427–429.
  10. Dzierzecka M, Kita J. The use of chosen serological diagnostic methods in Lyme disease in horses. Part I. Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA).. Pol J Vet Sci 2002;5:71–77.
    pubmed: 12189952
  11. Ebani V V, Bertelloni F, Pinzauti P, Cerri D. Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses.. Ann Agric Environ Med 2012;19:237–240.
    pubmed: 22742794
  12. Ebani V V. Serological evidence of Anaplasma phagocytophilum and spotted fever group Rickettsia spp. exposure in horses from central Italy.. Pathogens 2019;8:88.
    pmc: PMC6789761pubmed: 31247976
  13. Egenvall A, Franzén P, Gunnarsson A, Engvall E O, Vågsholm I, Wikström U B, Artursson K. Cross-sectional study of the seroprevalence to Borrelia burgdorferi sensu lato and granulocytic Ehrlichia spp and demographic, clinical and tick-exposure factors in Swedish horses.. Prev Vet Med 2001;49:191–208.
    pubmed: 11311953
  14. Elelu N. Tick-borne relapsing fever as a potential veterinary medical problem.. Vet Med Sci 2018;4:271–279.
    pmc: PMC6236141pubmed: 29943903
  15. Hermann M. Colitis X in the horse: 9 cases.. Schweiz Arch Tierheilkd 1985;127:385–396.
    pubmed: 4023687
  16. Imai D M, Barr B C, Daft B, Bertone J J, Feng S, Hodzic E, Johnston J M, Olsen K J, Barthold S W. Lyme neuroborreliosis in 2 horses.. Vet Pathol 2011;48:1151–1157.
    pubmed: 21285382
  17. Johnson A L, Divers T J, Chang Y F. Validation of an in-clinic enzyme-linked immunosorbent assay kit for diagnosis of Borrelia burgdorferi infection in horses.. J Vet Diagn Invest 2008;20:321–324.
    pubmed: 18460618
  18. Kiss T, Cadar D, Krupaci A F, Bordeanu A, Brudaşcă G F, Mihalca A D, Mircean V, Gliga L, Dumitrache M O, Spînu M. Serological reactivity to Borrelia burgdorferi sensu lato in dogs and horses from distinct areas in Romania.. Vector Borne Zoonotic Dis 2011;11:1259–1262.
    pubmed: 21612524
  19. Korbutiak E, Schneiders D. Equine granulocytic ehrlichiosis in the UK.. Vet Rec 1994;135:387–388.
    pubmed: 7831748
  20. Laus F, Veronesi F, Passamonti F, Paggi E, Cerquetella M, Hyatt D, Tesei B, Fioretti D P. Prevalence of tick borne pathogens in horses from Italy.. J Vet Med Sci 2013;75:715–720.
    pubmed: 23328633
  21. Leblond A, Pradier S, Pitel P H, Fortier G, Boireau P, Chadoeuf J, Sabatier P. Enquête épidémiologique sur l’anaplasmose équine Anaplasma phagocytophilum dans le Sud de la France.. Rev Sci Tech 2005;24:899–908.
    pubmed: 16642760
  22. Lee S H, Yun S H, Choi E, Park Y S, Lee S E, Cho G J, Kwon O D, Kwak D. Serological detection of Borrelia burgdorferi among horses in Korea.. Korean J Parasitol 2016;54:97–101.
    pmc: PMC4792323pubmed: 26951987
  23. Madigan J E, Gribble D. Equine ehrlichiosis in northern California: 49 cases (1968–1981). J Am Vet Med Assoc 1987;190:445–448.
    pubmed: 3558086
  24. Maloney E M, Lindenmayer J L. Seroprevalence and clinical signs of Lyme disease in Cape Cod horses.. Eq Pract 1992;14:15–19.
  25. Maurizi L, Marié J L, Aoun O, Courtin C, Gorsane S, Chal D, Davoust B. Seroprevalence survey of equine Lyme borreliosis in France and in sub-Saharan Africa.. Vector Borne Zoonotic Dis 2010;10:535–537.
    pubmed: 19877821
  26. Passamonti F, Veronesi F, Cappelli K, Capomaccio S, Reginato A, Miglio A, Vardi D M, Stefanetti V, Coletti M, Bazzica C, Pepe M. Polysynovitis in a horse due to Borrelia burgdorferi sensu lato infection – Case study.. Ann Agric Environ Med 2015;22:247–250.
    pubmed: 26094517
  27. Petrie A, Watson P. Statistics for Veterinary and Animal Science.. .
  28. Pusterla N, Madigan J E. Initial clinical impressions of the U.C. Davis large animal lift and its use in recumbent equine patients.. Schweiz Arch Tierheilkd 2006;148:161–166.
    pubmed: 16562727
  29. Saleem S, Ijaz M, Farooqi S H, Ghaffar A, Ali A, Iqbal K, Mehmood K, Zhang H. Equine granulocytic anaplasmosis 28 years later.. Microb Pathog 2018;119:1–8.
    pubmed: 29626656
  30. Saleem S, Ijaz M, Farooqi S H, Rashid M I, Khan A, Masud A, Aqib A I, Hussain K, Mehmood K, Zhang H. First molecular evidence of equine granulocytic anaplasmosis in Pakistan.. Acta Trop 2018;180:18–25.
    pubmed: 29306724
  31. Salinas-Mélendez J A, Galván de la Garza S, Riojas-Valdés V M, Wong González A, Avalos-Ramírez R. Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León.. Rev Latinoam Microbiol 2001;43:161–164.
    pubmed: 17061503
  32. Scarpulla M, Caristo M E, Macri G, Lillini E. Equine ehrlichiosis in Italy.. Ann N Y Acad Sci 2003;990:259–263.
    pubmed: 12860637
  33. Schvartz G, Epp T, Burgess H J, Chilton N B, Lohmann K L. Comparison between available serologic tests for detecting antibodies against Anaplasma phagocytophilum and Borrelia burgdorferi in horses in Canada.. J Vet Diagn Invest 2015;27:540–546.
    pubmed: 26069228
  34. Socoloski S N G, de Castro B G, Cordeiro M D, da Fonseca A H, Cepeda M B, Nicolino R R, Lopes L B. Epidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop-MT, Brazil.. Trop Anim Health Prod 2018;50:831–836.
    pubmed: 29388162
  35. Stefanciková A, Adaszek L, Pet'ko B, Winiarczyk S, Dudinák V. Serological evidence of Borrelia burgdorferi sensu lato in horses and cattle from Poland and diagnostic problems of Lyme borreliosis.. Ann Agric Environ Med 2008;15:37–43.
    pubmed: 18581977
  36. Thrusfield M, Ortega C, De Blas I, Noordhuizen J P, Frankena K. Win Episcope 2.0: improved epidemiological software for veterinary medicine.. Vet Rec 2001;148:567–572.
    pubmed: 11370882
  37. Tsachev I, Pantchev N, Marutsov P, Petrov V, Gundasheva D, Baymakova M. Serological evidence of Borrelia burgdorferi Anaplasma phagocytophilum and Ehrlichia spp. infections in horses from Southeastern Bulgaria.. Vector Borne Zoonotic Dis 2018;18:588–594.
    pubmed: 29969382
  38. Veronesi F, Laus F, Passamonti F, Tesei B, Piergili Fioretti D, Genchi C. Occurrence of Borrelia lusitaniae infection in horses.. Vet Microbiol 2012;160:535–538.
    pubmed: 22789679
  39. von Fricken M E, Sukhbaatar L, Boldbaatar B, Nymadawa P, Weppelmann T A, Baigalmaa B O, Anderson B D, Reller M E, Lantos P M, Gray G C. Estimated seroprevalence of Anaplasma spp. and spotted fever group Rickettsia exposure among herders and livestock in Mongolia.. Acta Trop 2018;177:179–185.
    pmc: PMC5671362pubmed: 29054570
  40. Wagner B, Erb H N. Dogs and horses with antibodies to outer-surface protein C as on-time sentinels for ticks infected with Borrelia burgdorferi in New York State in 2011.. Prev Vet Med 2012;107:275–279.
    pubmed: 22841496

Citations

This article has been cited 8 times.
  1. Ma W, Gao L, Wu X, Zhong L, Huang X, Yang R, Wu H, Zhu L, Ma W, Peng L, Li B, Song J, Luo S, Bao F, Liu A. Global prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum coinfection in wild and domesticated animals: A systematic review and meta-analysis. J Glob Health 2024 Dec 6;14:04231.
    doi: 10.7189/jogh.14.04231pubmed: 39641312google scholar: lookup
  2. Bogdan AM, Mitrea IL, Ionita M. Equine Granulocytic Anaplasmosis: A Systematic Review and Meta-Analysis on Clinico-Pathological Findings, Diagnosis, and Therapeutic Management. Vet Sci 2024 Jun 13;11(6).
    doi: 10.3390/vetsci11060269pubmed: 38922016google scholar: lookup
  3. Koutantou M, Drancourt M, Angelakis E. Prevalence of Lyme Disease and Relapsing Fever Borrelia spp. in Vectors, Animals, and Humans within a One Health Approach in Mediterranean Countries. Pathogens 2024 Jun 17;13(6).
    doi: 10.3390/pathogens13060512pubmed: 38921809google scholar: lookup
  4. Kašpárková N, Bártová E, Žákovská A, Budíková M, Sedlák K. Antibodies against Borrelia burgdorferi Sensu Lato in Clinically Healthy and Sick Horses: First Report from the Czech Republic. Microorganisms 2023 Jun 29;11(7).
    doi: 10.3390/microorganisms11071706pubmed: 37512879google scholar: lookup
  5. Abdelbaset AE, Kwak ML, Nonaka N, Nakao R. Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges. One Health 2023 Jun;16:100547.
    doi: 10.1016/j.onehlt.2023.100547pubmed: 37363219google scholar: lookup
  6. Zhang J, Ma H, Ai J, Qi T, Kang M, Li J, Sun Y. Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau. Animals (Basel) 2022 Oct 10;12(19).
    doi: 10.3390/ani12192723pubmed: 36230463google scholar: lookup
  7. Defaye B, Moutailler S, Pasqualini V, Quilichini Y. Distribution of Tick-Borne Pathogens in Domestic Animals and Their Ticks in the Countries of the Mediterranean Basin between 2000 and 2021: A Systematic Review. Microorganisms 2022 Jun 16;10(6).
    doi: 10.3390/microorganisms10061236pubmed: 35744755google scholar: lookup
  8. Villa L, Gazzonis AL, Allievi C, De Maria C, Persichetti MF, Caracappa G, Zanzani SA, Manfredi MT. Seroprevalence of Tick-Borne Infections in Horses from Northern Italy. Animals (Basel) 2022 Apr 12;12(8).
    doi: 10.3390/ani12080999pubmed: 35454246google scholar: lookup
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