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Acta veterinaria Scandinavica2016; 58(1); 38; doi: 10.1186/s13028-016-0215-6

Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction.

Abstract: It has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress. Oxidant-antioxidant imbalance is known to increase the conversion of deoxyguanosine to 8-hydroxy-2-deoxyguanosine (8-OHdG) in DNA. 8-OHdG can easily be measured using ELISA tests in serum or urine samples. In this study, we analysed serum 8-OHdG levels in horses with recurrent airway obstruction and in healthy controls. Results: The study material consisted of seven healthy horses and seven horses with symptomatic RAO. All horses were exposed to moldy hay and straw for 48 h to induce clinical exacerbation of RAO. The serum 8-OHdG levels were determined using the ELISA Highly Sensitive 8-OHdG kit. The difference between the levels of 8-OHdG in healthy and RAO-affected horses was significant. The median level of 8-OHdG was 0.044 ng/ml in the healthy controls versus 0.498 ng/ml in RAO horses (P = 0.0021). Conclusions: The results of the study strongly suggest that DNA damage coexists in the course of equine RAO. We therefore propose that future research should aim at the development of new drugs that target pro-inflammatory molecules, since DNA damage appears to be the result of chronic inflammation.
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Publication Date: 2016-06-07 PubMed ID: 27267076PubMed Central: PMC4895991DOI: 10.1186/s13028-016-0215-6Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examined the potential use of 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in horse’s serum as an indicator of DNA oxidative stress. This was tested in the context of horses suffering from recurrent airway obstruction (RAO), a condition known to increase oxidative stress.

Objective and Methodology

  • The authors of this research aimed to determine if serum levels of 8-OHdG – a substance produced when DNA undergoes damage due to oxidative stress – can act as an indicator of such stress in horses suffering from RAO.
  • The study included 14 horses, half of which were healthy and half of which suffered from symptomatic RAO.
  • All horses were exposed to hay and straw with mold – a factor known to exacerbate RAO symptoms – for a 48-hour period.
  • Following this period, the researchers measured serum 8-OHdG levels in all 14 horses using an ELISA Highly Sensitive 8-OHdG kit.

Findings and Conclusions

  • Analysis of the results indicated a significant difference between 8-OHdG levels in the two groups of horses.
  • The median level of 8-OHdG in the serum of healthy horses was 0.044 ng/ml, compared to a considerably higher 0.498 ng/ml in RAO-affected horses.
  • The significant difference in these concentrations led the researchers to conclude that DNA damage is present in horses suffering from RAO.
  • Finally, the authors recommend further research to develop drugs targeting pro-inflammatory molecules, as inflammation appears to be a significant contributor to the DNA damage observed in this study.

Cite This Article

APA
Niedzwiedz A, Borowicz H, Januszewska L, Markiewicz-Gorka I, Jaworski Z. (2016). Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction. Acta Vet Scand, 58(1), 38. https://doi.org/10.1186/s13028-016-0215-6

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 58
Issue: 1
Pages: 38
PII: 38

Researcher Affiliations

Niedzwiedz, Artur
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wrocław, Poland. artur.niedzwiedz@up.wroc.pl.
Borowicz, Hieronim
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wrocław, Poland.
Januszewska, Lidia
  • Department of Hygiene, Wroclaw Medical University, J. Mikulicza-Radeckiego 7, 50-435, Wrocław, Poland.
Markiewicz-Gorka, Iwona
  • Department of Hygiene, Wroclaw Medical University, J. Mikulicza-Radeckiego 7, 50-435, Wrocław, Poland.
Jaworski, Zbigniew
  • Department of Horse Breeding and Riding, University of Warmia and Mazury, Prawocheńskiego 2, 10-720, Olsztyn, Poland.

MeSH Terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Airway Obstruction / blood
  • Airway Obstruction / veterinary
  • Animals
  • Biomarkers / blood
  • DNA / chemistry
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Horse Diseases / blood
  • Horses
  • Inflammation / blood
  • Inflammation / veterinary
  • Oxidative Stress

References

This article includes 26 references
  1. Léguillette R. Recurrent airway obstruction--heaves.. Vet Clin North Am Equine Pract 2003 Apr;19(1):63-86, vi.
    doi: 10.1016/S0749-0739(02)00067-6pubmed: 12747662google scholar: lookup
  2. Bowler RP, Crapo JD. Oxidative stress in allergic respiratory diseases.. J Allergy Clin Immunol 2002 Sep;110(3):349-56.
    doi: 10.1067/mai.2002.126780pubmed: 12209079google scholar: lookup
  3. Deaton CM, Marlin DJ, Smith NC, Smith KC, Newton RJ, Gower SM, Cade SM, Roberts CA, Harris PA, Schroter RC, Kelly FJ. Breath condensate hydrogen peroxide correlates with both airway cytology and epithelial lining fluid ascorbic acid concentration in the horse.. Free Radic Res 2004 Feb;38(2):201-8.
    doi: 10.1080/10715160310001638047pubmed: 15104214google scholar: lookup
  4. Deaton CM, Marlin DJ, Deaton L, Smith NC, Harris PA, Schroter RC, Kelly FJ. Comparison of the antioxidant status in tracheal and bronchoalveolar epithelial lining fluids in recurrent airway obstruction.. Equine Vet J 2006 Sep;38(5):417-22.
    doi: 10.2746/042516406778400556pubmed: 16986601google scholar: lookup
  5. Kirschvink N, Marlin D, Delvaux F, Leemans J, Clercx C, Sparkes A, Gustin P. Collection of exhaled breath condensate and analysis of hydrogen peroxide as a potential marker of lower airway inflammation in cats.. Vet J 2005 May;169(3):385-96.
    doi: 10.1016/j.tvjl.2004.03.002pubmed: 15848781google scholar: lookup
  6. Art T, Kirschvink N, Smith N, Lekeux P. Indices of oxidative stress in blood and pulmonary epithelium lining fluid in horses suffering from recurrent airway obstruction.. Equine Vet J 1999 Sep;31(5):397-401.
    doi: 10.1111/j.2042-3306.1999.tb03839.xpubmed: 10505955google scholar: lookup
  7. Kirschvink N, Smith N, Fiévez L, Bougnet V, Art T, Degand G, Marlin D, Roberts C, Génicot B, Lindsey P, Lekeux P. Effect of chronic airway inflammation and exercise on pulmonary and systemic antioxidant status of healthy and heaves-affected horses.. Equine Vet J 2002 Sep;34(6):563-71.
    doi: 10.2746/042516402776180223pubmed: 12357995google scholar: lookup
  8. Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis.. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2009 Apr;27(2):120-39.
    doi: 10.1080/10590500902885684pubmed: 19412858google scholar: lookup
  9. Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics.. Clin Chim Acta 2004 Jan;339(1-2):1-9.
    doi: 10.1016/j.cccn.2003.09.010pubmed: 14687888google scholar: lookup
  10. Soini Y, Haapasaari KM, Vaarala MH, Turpeenniemi-Hujanen T, Kärjä V, Karihtala P. 8-hydroxydeguanosine and nitrotyrosine are prognostic factors in urinary bladder carcinoma.. Int J Clin Exp Pathol 2011 Mar;4(3):267-75.
    pmc: PMC3071659pubmed: 21487522
  11. Niedźwiedź A, Jaworski Z. Recurrent respiratory disorders in Polish Konik horses-clinical and laboratory findings. Bull Vet Inst Pulawy 2014;58:93–97.
  12. Wysocka B, Kluciński W. Usefulness of the assessment of discharge accumulation in the lower airways and tracheal septum thickening in the differential diagnosis of recurrent airway obstruction (RAO) and inflammatory airway disease (IAD) in the horse.. Pol J Vet Sci 2014;17(2):247-53.
    pubmed: 24988850doi: 10.2478/pjvs-2014-0035google scholar: lookup
  13. Fernandez NJ, Hecker KG, Gilroy CV, Warren AL, Léguillette R. Reliability of 400-cell and 5-field leukocyte differential counts for equine bronchoalveolar lavage fluid.. Vet Clin Pathol 2013 Mar;42(1):92-8.
    doi: 10.1111/vcp.12013pubmed: 23289790google scholar: lookup
  14. Niedzwiedz A, Jaworski Z. Oxidant-antioxidant status in the blood of horses with symptomatic recurrent airway obstruction (RAO).. J Vet Intern Med 2014 Nov-Dec;28(6):1845-52.
    doi: 10.1111/jvim.12452pmc: PMC4895620pubmed: 25231643google scholar: lookup
  15. Varani J, Ward PA. Mechanisms of neutrophil-dependent and neutrophil-independent endothelial cell injury.. Biol Signals 1994 Jan-Feb;3(1):1-14.
    doi: 10.1159/000109521pubmed: 7920971google scholar: lookup
  16. Ricciardolo FL, Di Stefano A, Sabatini F, Folkerts G. Reactive nitrogen species in the respiratory tract.. Eur J Pharmacol 2006 Mar 8;533(1-3):240-52.
    doi: 10.1016/j.ejphar.2005.12.057pubmed: 16464450google scholar: lookup
  17. Toussaint M, Fievez L, Desmet CJ, Pirottin D, Farnir F, Bureau F, Lekeux P. Increased hypoxia-inducible factor 1α expression in lung cells of horses with recurrent airway obstruction.. BMC Vet Res 2012 May 23;8:64.
    doi: 10.1186/1746-6148-8-64pmc: PMC3536633pubmed: 22621400google scholar: lookup
  18. Pacholewska A, Jagannathan V, Drögemüller M, Klukowska-Rötzler J, Lanz S, Hamza E, Dermitzakis ET, Marti E, Leeb T, Gerber V. Impaired Cell Cycle Regulation in a Natural Equine Model of Asthma.. PLoS One 2015;10(8):e0136103.
    doi: 10.1371/journal.pone.0136103pmc: PMC4546272pubmed: 26292153google scholar: lookup
  19. Araneda OF, Tuesta M. Lung oxidative damage by hypoxia.. Oxid Med Cell Longev 2012;2012:856918.
    doi: 10.1155/2012/856918pmc: PMC3433143pubmed: 22966417google scholar: lookup
  20. Miyata T, Takizawa S, van Ypersele de Strihou C. Hypoxia. 1. Intracellular sensors for oxygen and oxidative stress: novel therapeutic targets.. Am J Physiol Cell Physiol 2011 Feb;300(2):C226-31.
    doi: 10.1152/ajpcell.00430.2010pubmed: 20980551google scholar: lookup
  21. Fimognari C. Role of Oxidative RNA Damage in Chronic-Degenerative Diseases.. Oxid Med Cell Longev 2015;2015:358713.
    doi: 10.1155/2015/358713pmc: PMC4452857pubmed: 26078805google scholar: lookup
  22. Zeyrek D, Cakmak A, Atas A, Kocyigit A, Erel O. DNA damage in children with asthma bronchiale and its association with oxidative and antioxidative measurements.. Pediatr Allergy Immunol 2009 Jun;20(4):370-6.
    doi: 10.1111/j.1399-3038.2008.00780.xpubmed: 18801009google scholar: lookup
  23. Hasbal C, Aksu BY, Himmetoglu S, Dincer Y, Koc EE, Hatipoglu S, Akcay T. DNA damage and glutathione level in children with asthma bronchiale: effect of antiasthmatic therapy.. Pediatr Allergy Immunol 2010 Jun;21(4 Pt 2):e674-8.
    doi: 10.1111/j.1399-3038.2009.00959.xpubmed: 19840299google scholar: lookup
  24. Al-Afaleg NO, Al-Senaidy A, El-Ansary A. Oxidative stress and antioxidant status in Saudi asthmatic patients.. Clin Biochem 2011 Jun;44(8-9):612-7.
    doi: 10.1016/j.clinbiochem.2011.01.016pubmed: 21320478google scholar: lookup
  25. Bründler P, Frey CF, Gottstein B, Nussbaumer P, Neuhaus S, Gerber V. Lower shedding of strongylid eggs by Warmblood horses with recurrent airway obstruction compared to unrelated healthy horses.. Vet J 2011 Nov;190(2):e12-e15.
    doi: 10.1016/j.tvjl.2010.12.029pubmed: 21315626google scholar: lookup
  26. Neuhaus S, Bruendler P, Frey CF, Gottstein B, Doherr MG, Gerber V. Increased parasite resistance and recurrent airway obstruction in horses of a high-prevalence family.. J Vet Intern Med 2010 Mar-Apr;24(2):407-13.
    doi: 10.1111/j.1939-1676.2009.0465.xpubmed: 20102498google scholar: lookup

Citations

This article has been cited 6 times.
  1. Boni R, Cecchini Gualandi S. Relationship between Oxidative Stress and Endometritis: Exploiting Knowledge Gained in Mares and Cows. Animals (Basel) 2022 Sep 13;12(18).
    doi: 10.3390/ani12182403pubmed: 36139263google scholar: lookup
  2. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing. Animals (Basel) 2022 Mar 16;12(6).
    doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
  3. Zhang H, He J, Zhao Y, Wu Q, Zou T, Sun J, Zhu H, Wang X, Sun F, Xing J, Tang K. Effect of glutathione S-transferase gene polymorphisms on semen quality in patients with idiopathic male infertility. J Int Med Res 2021 Dec;49(12):3000605211061045.
    doi: 10.1177/03000605211061045pubmed: 34871528google scholar: lookup
  4. Davis KU, Sheats MK. The Role of Neutrophils in the Pathophysiology of Asthma in Humans and Horses. Inflammation 2021 Apr;44(2):450-465.
    doi: 10.1007/s10753-020-01362-2pubmed: 33150539google scholar: lookup
  5. Lendl L, Barton AK. Equine Asthma Diagnostics: Review of Influencing Factors and Difficulties in Diagnosing Subclinical Disease. Animals (Basel) 2024 Dec 4;14(23).
    doi: 10.3390/ani14233504pubmed: 39682469google scholar: lookup
  6. Palmisano M, Kulp J, Bender S, Stefanovski D, Robinson M, Johnson A. Measurement of 8-hydroxy-2'-deoxyguanosine in serum and cerebrospinal fluid of horses with neuroaxonal degeneration and other causes of proprioceptive ataxia. J Vet Intern Med 2024 Mar-Apr;38(2):1207-1213.
    doi: 10.1111/jvim.16988pubmed: 38205913google scholar: lookup
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