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Home / Equine Research Bank / Acta Vet Scand / Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidativ...
Acta veterinaria Scandinavica2016; 58(1); 38; doi: 10.1186/s13028-016-0215-6

Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction.

Abstract: It has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress. Oxidant-antioxidant imbalance is known to increase the conversion of deoxyguanosine to 8-hydroxy-2-deoxyguanosine (8-OHdG) in DNA. 8-OHdG can easily be measured using ELISA tests in serum or urine samples. In this study, we analysed serum 8-OHdG levels in horses with recurrent airway obstruction and in healthy controls. Results: The study material consisted of seven healthy horses and seven horses with symptomatic RAO. All horses were exposed to moldy hay and straw for 48 h to induce clinical exacerbation of RAO. The serum 8-OHdG levels were determined using the ELISA Highly Sensitive 8-OHdG kit. The difference between the levels of 8-OHdG in healthy and RAO-affected horses was significant. The median level of 8-OHdG was 0.044 ng/ml in the healthy controls versus 0.498 ng/ml in RAO horses (P = 0.0021). Conclusions: The results of the study strongly suggest that DNA damage coexists in the course of equine RAO. We therefore propose that future research should aim at the development of new drugs that target pro-inflammatory molecules, since DNA damage appears to be the result of chronic inflammation.
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Publication Date: 2016-06-07 PubMed ID: 27267076PubMed Central: PMC4895991DOI: 10.1186/s13028-016-0215-6Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examined the potential use of 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in horse’s serum as an indicator of DNA oxidative stress. This was tested in the context of horses suffering from recurrent airway obstruction (RAO), a condition known to increase oxidative stress.

Objective and Methodology

  • The authors of this research aimed to determine if serum levels of 8-OHdG – a substance produced when DNA undergoes damage due to oxidative stress – can act as an indicator of such stress in horses suffering from RAO.
  • The study included 14 horses, half of which were healthy and half of which suffered from symptomatic RAO.
  • All horses were exposed to hay and straw with mold – a factor known to exacerbate RAO symptoms – for a 48-hour period.
  • Following this period, the researchers measured serum 8-OHdG levels in all 14 horses using an ELISA Highly Sensitive 8-OHdG kit.

Findings and Conclusions

  • Analysis of the results indicated a significant difference between 8-OHdG levels in the two groups of horses.
  • The median level of 8-OHdG in the serum of healthy horses was 0.044 ng/ml, compared to a considerably higher 0.498 ng/ml in RAO-affected horses.
  • The significant difference in these concentrations led the researchers to conclude that DNA damage is present in horses suffering from RAO.
  • Finally, the authors recommend further research to develop drugs targeting pro-inflammatory molecules, as inflammation appears to be a significant contributor to the DNA damage observed in this study.

Cite This Article

APA
Niedzwiedz A, Borowicz H, Januszewska L, Markiewicz-Gorka I, Jaworski Z. (2016). Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction. Acta Vet Scand, 58(1), 38. https://doi.org/10.1186/s13028-016-0215-6

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 58
Issue: 1
Pages: 38
PII: 38

Researcher Affiliations

Niedzwiedz, Artur
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wrocław, Poland. artur.niedzwiedz@up.wroc.pl.
Borowicz, Hieronim
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wrocław, Poland.
Januszewska, Lidia
  • Department of Hygiene, Wroclaw Medical University, J. Mikulicza-Radeckiego 7, 50-435, Wrocław, Poland.
Markiewicz-Gorka, Iwona
  • Department of Hygiene, Wroclaw Medical University, J. Mikulicza-Radeckiego 7, 50-435, Wrocław, Poland.
Jaworski, Zbigniew
  • Department of Horse Breeding and Riding, University of Warmia and Mazury, Prawocheńskiego 2, 10-720, Olsztyn, Poland.

MeSH Terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Airway Obstruction / blood
  • Airway Obstruction / veterinary
  • Animals
  • Biomarkers / blood
  • DNA / chemistry
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Horse Diseases / blood
  • Horses
  • Inflammation / blood
  • Inflammation / veterinary
  • Oxidative Stress

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Citations

This article has been cited 4 times.
  1. Boni R, Cecchini Gualandi S. Relationship between Oxidative Stress and Endometritis: Exploiting Knowledge Gained in Mares and Cows.. Animals (Basel) 2022 Sep 13;12(18).
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  2. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
    doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
  3. Zhang H, He J, Zhao Y, Wu Q, Zou T, Sun J, Zhu H, Wang X, Sun F, Xing J, Tang K. Effect of glutathione S-transferase gene polymorphisms on semen quality in patients with idiopathic male infertility.. J Int Med Res 2021 Dec;49(12):3000605211061045.
    doi: 10.1177/03000605211061045pubmed: 34871528google scholar: lookup
  4. Davis KU, Sheats MK. The Role of Neutrophils in the Pathophysiology of Asthma in Humans and Horses.. Inflammation 2021 Apr;44(2):450-465.
    doi: 10.1007/s10753-020-01362-2pubmed: 33150539google scholar: lookup
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