Serum alkaline phosphatase isoenzymes in the horse–variation with age, training and in different pathological conditions.
Abstract: Serum alkaline Phosphatase isoenzyme (ALP) activity was measured by agarose gel electrophoresis in horses, Swedish half-breds and trotters, of varying age and during different conditions of training. Thus the effects of aging and growing on the isoenzyme activities from birth up to about 3 years of age was studied. The influence of training during the first year of life on the ALP isoenzyme pattern was also evaluated. Furthermore the isoenzyme pattern in adult horses suffering from different kinds of diseases was studied.
In newborn and very young horses normally two different isoenzyme fractions appear in the serum: liver and bone ALP. During the first year of life the bone isoenzyme fraction slowly disappears and in normal cases only liver ALP could be detected after the first year of life by the separation technique used in this study. In horses with different kinds of infectious and inflammatory diseases the liver ALP is often increased, which is probably a secondary phenomenon and related to an increased liver cell metabolism most likely caused by a general stress on the organism in different disease conditions.
Publication Date: 1988-01-01 PubMed ID: 3128022DOI: 10.1111/j.1439-0442.1988.tb00002.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper presents a study on the variations in ALP (alkaline phosphatase isoenzyme) levels in horses due to factors like age, training, and different diseases. It was found that activity of this enzyme changes with age, with distinct patterns identified in newborn/young horses and adult horses. Additionally, it was observed that horses with specific diseases showed increased levels of ALP.
Research Methodology
- The research team used agarose gel electrophoresis to measure ALP activity in Swedish half-bred and trotting horses of various ages and training statuses.
- They studied the effects of age and growth on these isoenzyme activities from birth up to 3 years of age.
- Additionally, the influence of training on the ALP isoenzyme patterns in horses during their first year of life was studied.
- The team also looked at the isoenzyme patterns in adult horses suffering from different types of diseases.
Findings on ALP Isoenzyme Activity in Horses
- Researchers found that in newborn and very young horses, two isoenzyme fractions (liver and bone ALP) typically appear in the blood serum.
- During the first year of life, the bone isoenzyme fraction gradually disappears. Normal samples do not show traces of the bone isoenzyme fraction after that first year, based on the separation technique used in the study.
- Generally, only liver ALP could be detected after the first year of life in horses.
- The levels of liver ALP were found to increase in horses suffering from different kinds of infectious and inflammatory diseases.
Implications of Findings
- The increase in liver ALP observed in diseased horses was described as a secondary phenomenon related to increased liver cell metabolism, which is likely triggered by general stress on the organism in disease conditions.
- These findings suggest that ALP activity could be used as a health marker in horses, shedding light on the state of their liver functioning or overall health, particularly in the presence of disease.
Cite This Article
APA
Thorén-Tolling K.
(1988).
Serum alkaline phosphatase isoenzymes in the horse–variation with age, training and in different pathological conditions.
Zentralbl Veterinarmed A, 35(1), 13-23.
https://doi.org/10.1111/j.1439-0442.1988.tb00002.x Publication
Researcher Affiliations
MeSH Terms
- Aging
- Alkaline Phosphatase / blood
- Animals
- Bone and Bones / enzymology
- Horses / blood
- Horses / physiology
- Isoenzymes / blood
- Liver / enzymology
- Physical Conditioning, Animal
Citations
This article has been cited 8 times.- Muñoz-Prieto A, Cerón JJ, Rubio CP, Contreras-Aguilar MD, Pardo-Marín L, Ayala-de la Peña I, Martín-Cuervo M, Holm Henriksen IM, Arense-Gonzalo JJ, Tecles F, Hansen S. Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome. Animals (Basel) 2022 Nov 23;12(23).
- Strand E, Braathen LC, Hellsten MC, Huse-Olsen L, Bjornsdottir S. Radiographic closure time of appendicular growth plates in the Icelandic horse. Acta Vet Scand 2007 Jul 17;49(1):19.
- Lepage OM, Marcoux M. Comparison of serum osteocalcin concentration and alkaline phosphatase activity as indicators of bone activity in a foal following surgery. Can Vet J 1991 Apr;32(4):239-40.
- Lepage OM, Marcoux M, Tremblay A. Serum osteocalcin or bone Gla-protein, a biochemical marker for bone metabolism in horses: differences in serum levels with age. Can J Vet Res 1990 Apr;54(2):223-6.
- Ellison RS, Jacobs RM. The isoelectric focusing properties of serum alkaline phosphatase in disease and following prednisolone and phenylbutazone administration in the horse. Can J Vet Res 1990 Jan;54(1):126-31.
- Ellison RS, Jacobs RM. An attempt to determine the tissue origin of equine serum alkaline phosphatase by isoelectric focusing. Can J Vet Res 1990 Jan;54(1):119-25.
- Lepage OM, DesCôteaux L, Marcoux M, Tremblay A. Circadian rhythms of osteocalcin in equine serum. Correlation with alkaline phosphatase, calcium, phosphate and total protein levels. Can J Vet Res 1991 Jan;55(1):5-10.
- Harari S, Deretz S, Dumont Saint Priest B, Richard E, Ricard A. Comparison of blood parameters in two genetically different groups of horses for functional longevity in show jumping. Front Genet 2024;15:1455790.
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