Serum keratan sulphate as a cartilage metabolic marker in horses: the effect of exercise.
Abstract: Keratan sulphate (KS) concentration in sera from resting horses and horses training daily on a racetrack was measured by an inhibition enzyme-linked immunosorbent assay (ELISA) using anti-equine KS antibody 1/14/16H9. For the in-training horses, serum KS concentrations in 2-year-old-horses was significantly higher than 3- or 4-year-old-horses. A higher concentration of serum KS was found in the in-training group than in the long-term resting group in 2-year-old-horses. Serum KS concentration increased remarkably immediately after training in healthy horses, and at 1, 5, 9 and 24 h after training remained at similar levels to the pre-training concentration. The results suggest that serum KS concentration could represent the situation of joint loading, induced by daily racetrack training, affecting the metabolic activities in joint cartilage.
Publication Date: 2002-06-19 PubMed ID: 12069261DOI: 10.1046/j.1439-0442.2002.00434.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research study assesses the impact of daily exercise on the concentration of keratan sulphate, a cartilage metabolic marker, in the blood of horses, revealing critical information for understanding the effect of rigorous physical activity on joint health in these animals.
Understanding the Objective
The primary intent of the research was to ascertain the effect of daily training or exercise on the concentration of keratan sulphate (KS), a biochemical marker associated with cartilage metabolism, in the blood serum of horses. This was done in order to understand the influence of intense physical activity, such as daily racetrack training, on their joint health.
Methodology Implemented
- The researchers conducted an inhibition enzyme-linked immunosorbent assay (ELISA) to measure the concentration of KS in the blood serum from both resting horses and those involved in daily training on a racetrack.
- The assay was performed using an anti-equine KS antibody. KS concentrations were evaluated in horses of different ages- 2, 3, and 4 years.
Key Findings
- The study discovered that the concentration of KS in the blood serum of horses training daily was significantly higher in the 2-year-old horses as compared to the 3- or 4-year-old horses.
- Furthermore, in the 2-year-old category, the active horses showed a higher concentration of serum KS than their resting counterparts.
- Moreover, the concentration of KS in the blood serum of the active horses increased significantly immediately after the exercise routine, but eventually returned to its pre-training level within 1 to 24 hours of the exercise.
Conclusions Drawn
The research concluded that blood serum concentration of keratan sulphate could be a potential indicator of the joint loading in horses resulting from daily racetrack training. Essentially, regular and intense physical activity seems to influence the metabolic activities in their joint cartilage, which might have implications on their joint health.
Cite This Article
APA
Okumura M, Kim GH, Tagami M, Haramaki S, Fujinaga T.
(2002).
Serum keratan sulphate as a cartilage metabolic marker in horses: the effect of exercise.
J Vet Med A Physiol Pathol Clin Med, 49(4), 195-197.
https://doi.org/10.1046/j.1439-0442.2002.00434.x Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. okumuram@vetmed.hokudai.ac.jp
MeSH Terms
- Age Factors
- Animals
- Biomarkers / blood
- Cartilage, Articular / metabolism
- Enzyme-Linked Immunosorbent Assay / methods
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Horses / blood
- Keratan Sulfate / analysis
- Keratan Sulfate / blood
- Male
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 3 times.- Matheson A, Regmi SC, Jay GD, Schmidt TA, Scott WM. The Effect of Intense Exercise on Equine Serum Proteoglycan-4/Lubricin.. Front Vet Sci 2020;7:599287.
- Much ML, Leatherwood JL, Martinez RE, Silvers BL, Basta CF, Gray LF, Bradbery AN. Evaluation of an oral joint supplement on gait kinematics and biomarkers of cartilage metabolism and inflammation in mature riding horses.. Transl Anim Sci 2020 Jul;4(3):txaa150.
- Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses.. Oxid Med Cell Longev 2012;2012:920932.
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