Serum level of cartilage oligomeric matrix protein (COMP) in equine osteoarthritis.
Abstract: This study was designed to assay and compare cartilage oligomeric matrix protein (COMP) in horse sera, in samples from normal and joint diseased horses, and to investigate the relationships between COMP in sera and synovial fluids (SF) with keratan sulphate (KS) data. Sera from 38 horses free of any joint pathology (controls) and from horses with aseptic joint disease (AJD horses, n = 40) were assayed for COMP and KS concentrations. Of the 78 horses in the study, 53 were also assayed for COMP and KS concentrations in SF. COMP and KS were measured by inhibition ELISA, using monoclonal antibodies 12C4 and 5D4, respectively. The COMP concentration in sera from AJD horses (mean +/- s.d. 10.7 +/- 7.4 microg/ml) was significantly (P<0.02) lower than in control sera (14.8 +/- 7.8 microg/ml). The joint disease sera also had significantly lower (P<0.01) KS levels (180.5 +/- 61.8 ng/ml) than controls (237.1 +/- 116.1 ng/ml). A significant correlation (r = 0.52, n = 53, P<0.001) was seen between serum and SF in COMP levels; no such relationship was seen in KS levels. It is possible that serum COMP concentration could be a more specific marker of equine joint disease than any other described to date.
Publication Date: 2002-10-03 PubMed ID: 12358001DOI: 10.2746/042516402776180205Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Biochemistry
- Biomarkers
- Cartilage
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Horses
- Inflammation
- Joint Health
- Monoclonal Antibodies
- Osteoarthritis
- Proteoglycans
- Serum
- Synovial Fluid
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research conducted a comparative examination of the concentrations of cartilage oligomeric matrix protein (COMP) in the blood serum of horses, specifically between those with healthy joints and those suffering from joint disease, and assessed the potential of COMP as a marker for equine joint disease.
Research Method
- The study involved a total of 78 horses, 38 of which showed no signs of joint pathology and served as control, and 40 that were diagnosed with aseptic joint disease (AJD).
- The researchers measured the quantity of COMP and keratan sulfate (KS), another biomarker, in both the blood serum and synovial fluids (the fluid in the joint space) of the horses.
- The measuring of COMP and KS was done through inhibition Enzyme-Linked ImmunoSorbent Assay (ELISA), a procedure that establishes the concentration of molecules, using distinctive monoclonal antibodies.
Findings and Interpretation
- Results indicated that horses with AJD had significantly lesser COMP in their serum as compared to healthy horses.
- There was also lower presence of KS in the serum of horses with joint disease compared to the control group.
- There was a notable correlation between the COMP levels in serum and synovial fluids, suggesting the fluctuations of COMP in serum mirror those in the joint space.
- No substantial correlation was found between the KS levels in serum and synovial fluids.
Significance and Implication
- Based on the relationships identified between serum and synovial fluid COMP levels, and the difference in COMP levels between healthy and diseased horses, it is inferred that COMP concentration in the serum could indicate the state of joint health in horses.
- This discovery suggests that blood serum COMP concentration could be utilized as an indicator or marker for equine joint disease, potentially more accurately than other markers currently in use.
Cite This Article
APA
Misumi K, Vilim V, Hatazoe T, Murata T, Fujiki M, Oka T, Sakamoto H, Carter SD.
(2002).
Serum level of cartilage oligomeric matrix protein (COMP) in equine osteoarthritis.
Equine Vet J, 34(6), 602-608.
https://doi.org/10.2746/042516402776180205 Publication
Researcher Affiliations
- Department of Veterinary Medicine, Kagoshima University, Japan.
MeSH Terms
- Animals
- Antibodies, Monoclonal / analysis
- Biomarkers / blood
- Blotting, Western / veterinary
- Case-Control Studies
- Electrophoresis, Polyacrylamide Gel / veterinary
- Enzyme-Linked Immunosorbent Assay / veterinary
- Extracellular Matrix Proteins / blood
- Glycoproteins / blood
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Joint Diseases / blood
- Joint Diseases / diagnosis
- Joint Diseases / veterinary
- Keratan Sulfate / blood
- Matrilin Proteins
- Osteoarthritis / blood
- Osteoarthritis / diagnosis
- Osteoarthritis / veterinary
- Synovial Fluid / chemistry
Citations
This article has been cited 9 times.- Smith R, Önnerfjord P, Holmgren K, di Grado S, Dudhia J. Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease.. Int J Mol Sci 2020 Mar 20;21(6).
- Turlo AJ, Cywinska A, Frisbie DD. Revisiting predictive biomarkers of musculoskeletal injury in thoroughbred racehorses: longitudinal study in polish population.. BMC Vet Res 2019 Feb 26;15(1):66.
- Ekman S, Lindahl A, Rüetschi U, Jansson A, Björkman K, Abrahamsson-Aurell K, Björnsdóttir S, Löfgren M, Hultén LM, Skiöldebrand E. Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses.. Equine Vet J 2019 Sep;51(5):674-680.
- Lin EA, Liu CJ. The emerging roles of ADAMTS-7 and ADAMTS-12 matrix metalloproteinases.. Open Access Rheumatol 2009;1:121-131.
- Zhang Y, Wei F, Liu CJ. Overexpression of ADAMTS-7 leads to accelerated initiation and progression of collagen-induced arthritis in mice.. Mol Cell Biochem 2015 Jun;404(1-2):171-9.
- Liu CJ. Progranulin: a promising therapeutic target for rheumatoid arthritis.. FEBS Lett 2011 Dec 1;585(23):3675-80.
- Lin EA, Liu CJ. The role of ADAMTSs in arthritis.. Protein Cell 2010 Jan;1(1):33-47.
- Kong L, Tian Q, Guo F, Mucignat MT, Perris R, Sercu S, Merregaert J, Di Cesare PE, Liu CJ. Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth.. Matrix Biol 2010 May;29(4):276-86.
- Liu CJ, Kong W, Ilalov K, Yu S, Xu K, Prazak L, Fajardo M, Sehgal B, Di Cesare PE. ADAMTS-7: a metalloproteinase that directly binds to and degrades cartilage oligomeric matrix protein.. FASEB J 2006 May;20(7):988-90.
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