Serum markers of lamellar basement membrane degradation and lamellar histopathological changes in horses affected with laminitis.
Abstract: In order better to evaluate the extent to which degradation of the lamellar basement membrane (LBM) by matrix metalloproteinases (MMP) occurs in equine laminitis, we determined the concentration of type IV collagen and laminin in normal and laminitic horses, using specific immunoassays. Blood samples were obtained from both the jugular and the cephalic veins of horses (n = 10) before and after the induction of acute alimentary laminitis by carbohydrate overload. Jugular and cephalic venous blood samples were also obtained from horses affected with naturally occurring laminitis (n = 16) and nonlaminitic controls (n = 8). The serum collagen IV concentration was not changed following the induction of laminitis in the experimental group. Serum collagen IV concentration was increased in jugular venous blood obtained from cases of naturally occurring laminitis (mean +/- s.e. 218.04 +/- 18.59 ng/ml) compared with nonlaminitic controls (157.50 +/- 10.93 ng/ml) (P<0.05). Serum collagen IV concentration was also increased in jugular venous blood obtained from severely laminitic horses (219.50 +/- 18.18 ng/ml) compared with nonlaminitic controls (157.50 +/- 10.93 ng/ml) (P<0.05). A difference in serum concentration of collagen IV was not identified based on chronicity of naturally occurring laminitis. Serum laminin concentration did not differ between laminitic and nonlaminitic horses. Differences in serum laminin concentration were not identified based on sampling location (jugular or cephalic vein), severity of laminitic pain, or chronicity of spontaneous laminitis. In conclusion, the circulating concentration of collagen IV was increased in horses affected with naturally occurring laminitis. The potential role for serum collagen IV assay for characterisation of equine laminitis warrants further investigation.
Publication Date: 2000-11-28 PubMed ID: 11093618DOI: 10.2746/042516400777584695Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study aimed to measure the degradation of a certain structural element in horse hooves, called the lamellar basement membrane (LBM), in horses affected by laminitis. The researchers used specific tests to measure the levels of type IV collagen and laminin in both healthy and laminitic horses.
Objectives and Methodology
- This research aimed to assess the extent of degradation of the LBM—a thin sheet-like structure important for maintaining tissue structure—in horses affected by laminitis, a painful and disabling condition affecting horse’s feet.
- The research focused on the role of matrix metalloproteinases (MMPs), enzymes that play a significant role in the degradation of the LBM.
- The researchers measured the concentration of two proteins—type IV collagen and laminin, which are key components of the LBM—in both healthy nonlaminitic and laminitic horses.
- Their methodology involved sampling blood from 10 horses, before and after inducing laminitis through a carbohydrate overload, as well as from 16 horses displaying naturally occurring laminitis and 8 healthy controls.
Results of the Study
- The concentration of collagen IV, a component of the LBM, did not change after inducing laminitis experimentally.
- However, increased collagen IV levels were noted in the blood from jugular veins of naturally affected laminitic horses, compared to control horses.
- Interestingly, the chronicity of naturally occurring laminitis didn’t affect the collagen IV concentration in a significant manner.
- The concentration of another LBM component, laminin, showed no differences between laminitic and nonlaminitic horses.
- No significant differences were observed in laminin concentration due to the location of blood sampling, severity of laminitic pain, or chronicity of spontaneous laminitis.
Conclusions
- The findings suggest an increased presence of collagen IV in the blood circulation of horses suffering from naturally occurring laminitis.
- The results thus indicate a potential role for the assay or examination of serum (blood) collagen IV in the evaluation, and possibly diagnosis, of equine laminitis.
- The researchers, therefore, recommend further investigation of the role of serum collagen IV assay in characterising equine laminitis.
Cite This Article
APA
Johnson PJ, Kreeger JM, Keeler M, Ganjam VK, Messer NT.
(2000).
Serum markers of lamellar basement membrane degradation and lamellar histopathological changes in horses affected with laminitis.
Equine Vet J, 32(6), 462-468.
https://doi.org/10.2746/042516400777584695 Publication
Researcher Affiliations
- Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
MeSH Terms
- Animals
- Basement Membrane / pathology
- Biomarkers
- Collagen / blood
- Female
- Foot Diseases / pathology
- Foot Diseases / veterinary
- Hoof and Claw
- Horse Diseases / blood
- Horse Diseases / pathology
- Horses
- Immunoassay / veterinary
- Lameness, Animal / pathology
- Laminin / blood
- Male
Citations
This article has been cited 7 times.- Yang Q, Lopez MJ. Ultrastructural morphology is distinct among primary progenitor cell isolates from normal, inflamed, and cryopreserved equine hoof tissue and CD105(+)K14(+) progenitor cells.. In Vitro Cell Dev Biol Anim 2019 Sep;55(8):641-655.
- Wang L, Pawlak EA, Johnson PJ, Belknap JK, Alfandari D, Black SJ. Expression and activity of collagenases in the digital laminae of horses with carbohydrate overload-induced acute laminitis.. J Vet Intern Med 2014 Jan-Feb;28(1):215-22.
- Wang L, Pawlak EA, Johnson PJ, Belknap JK, Eades S, Stack S, Cousin H, Black SJ. Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae.. PLoS One 2013;8(2):e56025.
- Wang L, Pawlak E, Johnson PJ, Belknap JK, Alfandari D, Black SJ. Effects of cleavage by a disintegrin and metalloproteinase with thrombospondin motifs-4 on gene expression and protein content of versican and aggrecan in the digital laminae of horses with starch gruel-induced laminitis.. Am J Vet Res 2012 Jul;73(7):1047-56.
- Pawlak E, Wang L, Johnson PJ, Nuovo G, Taye A, Belknap JK, Alfandari D, Black SJ. Distribution and processing of a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecan, versican, and hyaluronan in equine digital laminae.. Am J Vet Res 2012 Jul;73(7):1035-46.
- Hayden MR, Habibi J, Joginpally T, Karuparthi PR, Sowers JR. Ultrastructure Study of Transgenic Ren2 Rat Aorta - Part 1: Endothelium and Intima.. Cardiorenal Med 2012 Feb;2(1):66-82.
- Coyne MJ, Cousin H, Loftus JP, Johnson PJ, Belknap JK, Gradil CM, Black SJ, Alfandari D. Cloning and expression of ADAM-related metalloproteases in equine laminitis.. Vet Immunol Immunopathol 2009 Jun 15;129(3-4):231-41.
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