Serum osteocalcin in donkeys as evaluated with an equine-specific radioimmunoassay.
Abstract: The purpose of this study was to validate an equine-specific osteocalcin (OC) radioimmunoassay (RIA) for use in donkeys and to establish age-related changes in serum OC concentrations in healthy donkeys. Serial dilutions of donkey serum showed parallelism with standard curves obtained with the equine-specific OC RIA. There was a tight linear regression between donkey serum OC values obtained with the equine specific OC RIA and a commercially available bovine-specific OC RIA. Serum OC levels of 27 healthy donkeys, analysed with the equine-specific OC RIA, showed a tight negative logarithmic regression with age. Least square means and standard error of serum OC values were 67 +/- 10 microg/l in 0.6-4-year-old donkeys (group 1), 32 +/- 9 microg/l in 5-9-year-old donkeys (group 2) and 15 +/- 11 microg/l in > 9-year-old donkeys (group 3). Serum OC values were significantly (p < 0.01) higher in group 1 than in groups 2 and 3. The equine-specific OC RIA may be a useful and practical tool to assess bone metabolism and skeletal diseases in donkeys.
Publication Date: 2004-02-01 PubMed ID: 19774757DOI: 10.1111/j.1439-0396.2004.00443.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigated the use of a horse-specific osteocalcin (OC) radioimmunoassay (RIA) in donkeys and identified age-related changes in OC levels in the blood of healthy donkeys.
Study Objective
- The primary objective of this research was to ascertain the validity of an equine-specific osteocalcin (OC) radioimmunoassay (RIA) for use with donkeys. Another goal was to categorize any age-related changes in serum osteocalcin concentrations in healthy donkeys.
Methods
- To carry out this experiment, the researchers used serial dilutions of donkey serum. These were compared with standard curves obtained from using the equine-specific OC RIA.
- A close linear regression was found between donkey serum OC values measured by the equine specific OC RIA and a commercial bovine-specific OC RIA, showing the compatibility of the two assays.
- The testers then analyzed serum OC levels of 27 healthy donkeys using the equine-specific OC RIA and their ages were taken into account.
Results
- The results highlighted a strong negative logarithmic regression with age in relation to serum OC levels in donkeys when analysed with the equine-specific OC RIA.
- It was found that younger donkeys (0.6-4 years old – group 1) had the highest levels of OC with average serum OC values of 67 +/- 10 microg/l. Mid-age donkeys (5-9 years old – group 2) had OC levels of 32 +/- 9 microg/l and older donkeys (> 9 years old – group 3) had the least amounts at 15 +/- 11 microg/l.
- The results showed that OC levels were significantly (p < 0.01) higher in the youngest age group (group 1) compared to groups 2 and 3.
Conclusion
- The study establishes that the equine-specific OC RIA is a potential useful tool for the assessment of bone metabolism and skeletal diseases in donkeys. This conclusion is based on the successful validation of the equine-specific RIA for use in donkeys, and the distinct age-specific differences in serum OC levels among the donkeys tested.
Cite This Article
APA
Carstanjen B, Amory H, Youssao I, Remy B.
(2004).
Serum osteocalcin in donkeys as evaluated with an equine-specific radioimmunoassay.
J Anim Physiol Anim Nutr (Berl), 88(1-2), 1-6.
https://doi.org/10.1111/j.1439-0396.2004.00443.x Publication
Researcher Affiliations
- Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
MeSH Terms
- Age Distribution
- Aging
- Animals
- Equidae / blood
- Female
- Male
- Osteocalcin / blood
- Radioimmunoassay / methods
- Radioimmunoassay / veterinary
- Reproducibility of Results
- Sex Characteristics
Citations
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