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Home / Equine Research Bank / J Vet Med Sci / Serum protein expression in Equine Glandular Gastric Disease...
The Journal of veterinary medical science2019; 81(3); 418-424; doi: 10.1292/jvms.18-0679

Serum protein expression in Equine Glandular Gastric Disease (EGGD) induced by phenylbutazone.

Abstract: Equine Glandular Gastric Disease (EGGD) is a common disease in sport horses. This disease might be associated with usage of nonsteroidal anti-inflammatory drugs (NSAIDs) for treating inflammatory diseases. Although gastroscopy has been an effective method for diagnosis, but a less invasive, and inexpensive method is preferred. This study used proteomic technology to identify candidate serum proteins that might be used as markers of NSAIDs induced EGGD. Five Thoroughbred horses were given high doses of NSAID, phenylbutazone to treat lameness. The experiment was divided into three periods: (i) Pre-EGGD period, (ii) during EGGD period, and (iii) Post-EGGD period. Gastroscopy were used to diagnose EGGD, serum was collected to perform gel electrophoresis (1D SDS-PAGE) and mass spectrometry (LC-MS) in order to identify serum proteins in each group. The candidate serum proteins were computationally predicted for the interaction between phenylbutazone and proteins, tissue specific expression, and association to gastric ulceration. After EGGD induction, all horses showed clinical signs of colic with marked congestion and erosion appearing in the mucosa of the glandular stomach whereas no change was observed in the mucosa of non-glandular stomach. Our proteomic results identified 14 proteins that might be used as EGGD markers. These proteins were highly expressed in the glandular stomach and some proteins were associated with phenylbutazone or ulcer development. However, confirmation of these candidate marker proteins is required with specific antibodies in the larger horse population before they can be considered for application in the field.
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Publication Date: 2019-01-23 PubMed ID: 30674748PubMed Central: PMC6451916DOI: 10.1292/jvms.18-0679Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper studies the connection between nonsteroidal anti-inflammatory drugs (NSAIDs) and Equine Glandular Gastric Disease (EGGD) in sport horses, using proteomic technologies to identify possible biomarkers for the disease.

Introduction

  • The study is framed around the prevalence of Equine Glandular Gastric Disease (EGGD) in sport horses, and how this condition may be associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs) for treating inflammatory diseases.
  • The research identifies a need for a less invasive and less expensive method of diagnosing EGGD, as the established method, gastroscopy, may not be ideal in all cases.
  • To this end, the study sets out to use proteomic technology in order to identify candidate proteins in the serum of the horses that could function as markers of NSAID-induced EGGD.

Methodology

  • The researchers used five Thoroughbred horses, treating them with high doses of the NSAID, phenylbutazone, used for lameness.
  • They structured the experiment to happen in three periods – pre-EGGD, during EGGD, and post-EGGD – using gastroscopy to diagnose the disease and collecting serum at each stage.
  • They then carried out gel electrophoresis (1D SDS-PAGE) and mass spectrometry (LC-MS) on the serum samples to identify the proteins present during each stage.
  • In order to identify possible marker proteins, they predicted computationally the interaction between phenylbutazone and the proteins, the tissue specific expression, and the association to gastric ulceration.

Results

  • After inducing EGGD, all horses showed symptoms of colic, with visible congestion and erosion in the stomach’s glandular mucosa; the non-glandular stomach mucosa remained unaffected.
  • The proteomic analysis identified 14 proteins that could potentially be used as markers for EGGD.
  • These proteins were prominently expressed in the glandular stomach, and some of them were associated either with phenylbutazone, or with the development of ulcers.

Conclusion

  • The study concluded by acknowledging that, while these 14 identified proteins could serve as EGGD markers, further research is required to confirm these findings.
  • This additional research would involve testing the candidate marker proteins with specific antibodies in a larger horse population.
  • Only after this additional validation could these candidate marker proteins potentially be used as diagnostic tools in real-world applications.

Cite This Article

APA
Tesena P, Yingchutrakul Y, Roytrakul S, Wongtawan T, Angkanaporn K. (2019). Serum protein expression in Equine Glandular Gastric Disease (EGGD) induced by phenylbutazone. J Vet Med Sci, 81(3), 418-424. https://doi.org/10.1292/jvms.18-0679

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 81
Issue: 3
Pages: 418-424

Researcher Affiliations

Tesena, Parichart
  • Graduate Program in Animal Physiology, Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  • Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom 73170, Thailand.
Yingchutrakul, Yodying
  • Proteomics Research Laboratory, Genome Technology Research Unit, National Center for Genetics Engineering and Biotechnology, Pathum Thani 12120, Thailand.
Roytrakul, Sittiruk
  • Proteomics Research Laboratory, Genome Technology Research Unit, National Center for Genetics Engineering and Biotechnology, Pathum Thani 12120, Thailand.
Wongtawan, Tuempong
  • Department of Pre-clinical and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom 73170, Thailand.
Angkanaporn, Kris
  • Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

MeSH Terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / toxicity
  • Blood Proteins / genetics
  • Blood Proteins / metabolism
  • Horse Diseases / chemically induced
  • Horses
  • Male
  • Phenylbutazone / toxicity
  • Stomach Diseases / chemically induced

Conflict of Interest Statement

The authors declare no conflict of interest.

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