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Serum quality: an analysis of its components.

Abstract: Foetal and new born bovine sera, horse serum, human serum and human plasma, and protein solutions prepared from the by-products of human plasma fractionation have been analysed. Foetal bovine sera were found to have lower total protein (g/l) and % of gamma-globulin than the other sera studied while the potassium (mmol/1) was higher. Protease inhibitors could be detected in all specimens tested.
Publication Date: 1980-01-01 PubMed ID: 6154000
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  • Comparative Study
  • Journal Article

Summary

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This research article studies and analyzes the components of various types of sera: foetal and new born bovine (calf) serum, horse serum, human serum and human plasma, and protein solutions made from human plasma by-products.

Study of Different Types of Sera

The research conducted aimed at understanding the various components of different types of sera. The subjects of this study included:

  • Foetal and newborn bovine sera
  • Horse serum
  • Human serum and human plasma
  • Protein solutions prepared from by-products of human plasma fractionation

Comparison of Components in Different Sera

During the analysis, the research found differing levels of certain compounds in the sera.

  • Foetal bovine sera had lower total protein (g/l) and gamma-globulin percent than the other types of sera studied. In simpler terms, newborn calf blood had lower levels of total proteins and a specific type of protein (gamma-globulin) when compared to the others.
  • Potassium levels (mmol/1) were found to be higher in foetal bovine sera. This indicates that the blood of newborn calves contained more potassium than the other sera in the study.

Detection of Protease Inhibitors

All samples tested in this study were found to contain protease inhibitors. Protease inhibitors are substances that block the activity of proteases, enzymes that break down proteins. This means that, in all the different types of sera examined, elements were present that prevent the breakdown of proteins. This detail regarding the presence of protease inhibitors broadens our understanding of the constituents and functionality of these sera.

Cite This Article

APA
Macleod AJ, Drummond O. (1980). Serum quality: an analysis of its components. Dev Biol Stand, 46, 17-20.

Publication

ISSN: 0301-5149
NlmUniqueID: 0427140
Country: Switzerland
Language: English
Volume: 46
Pages: 17-20

Researcher Affiliations

Macleod, A J
    Drummond, O

      MeSH Terms

      • Animals
      • Blood Chemical Analysis
      • Blood Proteins / analysis
      • Cattle
      • Horses
      • Humans
      • Plasma / analysis
      • Potassium / analysis
      • Serum Albumin / analysis
      • alpha 1-Antitrypsin / analysis
      • gamma-Globulins / analysis

      Citations

      This article has been cited 1 times.
      1. Price PJ, Gregory EA. Relationship between in vitro growth promotion and biophysical and biochemical properties of the serum supplement. In Vitro 1982 Jun;18(6):576-84.
        doi: 10.1007/BF02810081pubmed: 7118138google scholar: lookup