Sevoflurane inhibits equine myeloperoxidase release and activity in vitro.
Abstract: To investigate the effects of the volatile anaesthetic sevoflurane on the release of total and active myeloperoxidase (MPO) by non-stimulated and stimulated polymorphonuclear neutrophils (PMNs) in whole blood from healthy horses. Methods: In vitro experimental study. Methods: Adult healthy horses. Methods: Samples of whole venous blood were collected and incubated in air or in air plus 2.3% or 4.6% sevoflurane for 1 hour. PMNs were stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP), with a combination of cytochalasin B (CB) and fMLP or with phorbol myristate acetate (PMA). Total and active MPO contents released by PMNs in blood were measured by enzyme-linked immunosorbent assay (ELISA) and specific immunological extraction followed by enzymatic detection (SIEFED) respectively. Additional experiments were performed to assess the effect of sevoflurane on the peroxidase and chlorination cycles of purified equine MPO using Amplex Red and 3'-(p-aminophenyl) fluorescein as fluorogenic substrates respectively. Results: As compared with air alone, 1 hour exposure of whole blood to 4.6% sevoflurane in air significantly inhibited the release of total and active MPO by unstimulated and both fMLP- and CB + fMLP-stimulated PMNs but not by PMA-stimulated PMNs. Although 2.3% sevoflurane had no effect on total MPO release by unstimulated and stimulated PMNs, it significantly reduced the release of active MPO by unstimulated and fMLP-stimulated PMNs. Additionally, sevoflurane reversibly inhibited the activity of MPO, especially the peroxidase cycle of the enzyme. Conclusions: Although our experimental study was not designed to assess the effects of sevoflurane in vivo, this inhibition of MPO release and activity may have relevance for anaesthetized horses and deserves further studies to examine the clinical importance of these findings.
© 2012 The Authors. Veterinary Anaesthesia and Analgesia. © 2012 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesiologists.
Publication Date: 2012-10-11 PubMed ID: 23057425DOI: 10.1111/j.1467-2995.2012.00786.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The study explores how the anaesthetic sevoflurane affects the release and activity of myeloperoxidase, an enzyme in white blood cells, in healthy horses. The findings suggest that sevoflurane can inhibit the release and activity of this enzyme, which could potentially have implications during horse anaesthesia.
Experiment Design and Methodology
- The research team conducted an in vitro experimental study using blood samples from healthy adult horses.
- The blood samples were incubated in air or with two different concentrations of sevoflurane (2.3% and 4.6%) for an hour.
- The white blood cells, specifically polymorphonuclear neutrophils (PMNs), in these samples were then stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP), a combination of cytochalasin B (CB) and fMLP, or with phorbol myristate acetate (PMA).
- The team measured both the total and active myeloperoxidase (MPO) enzymes released by these cells using enzyme-linked immunosorbent assay (ELISA) and specific immunological extraction followed by enzymatic detection (SIEFED) respectively.
- Additional tests were done to see how sevoflurane affected the cycles of the MPO enzyme using specific substrates.
Key Results
- Compared with air alone, a one-hour exposure to 4.6% sevoflurane proved significant in inhibiting the release of both total and active MPO. This was observed in both unstimulated PMNs and those stimulated using fMLP and CB + fMLP. The effect was not registered with PMA-stimulated PMNs.
- At the lower concentration of 2.3%, sevoflurane had no effect on the total MPO release but reduced the release of active MPO in unstimulated and fMLP-stimulated PMNs.
- Sevoflurane also inhibited the activity of the MPO enzyme, indicating its potential effect on the overall function of the enzyme.
Potential Implications
- The results of this particular study suggest that sevoflurane could likely affect the release and activity of the MPO enzyme during anesthesia, which needs further study and clinical validation.
- Although designed as an experimental in vitro study, this research suggests that the use of sevoflurane in anesthetic procedures in horses might have biological effects that could impact the overall effectiveness or safety of anesthesia in this animal.
Cite This Article
APA
Minguet G, de la Rebière G, Franck T, Joris J, Serteyn D, Sandersen C.
(2012).
Sevoflurane inhibits equine myeloperoxidase release and activity in vitro.
Vet Anaesth Analg, 40(2), 166-175.
https://doi.org/10.1111/j.1467-2995.2012.00786.x Publication
Researcher Affiliations
- Department of Anaesthesia and Intensive Care Medicine, CHU de Liège, Domaine Universitaire du Sart-Tilman, Liège, Belgium. gminguet@chu.ulg.ac.be
MeSH Terms
- Animals
- Horses / blood
- Methyl Ethers / pharmacology
- Neutrophils / drug effects
- Neutrophils / enzymology
- Peroxidase / antagonists & inhibitors
- Platelet Aggregation Inhibitors / pharmacology
- Sevoflurane
Citations
This article has been cited 1 times.- Minguet G, Franck T, Joris J, Serteyn D. Sevoflurane modulates the release of reactive oxygen species, myeloperoxidase, and elastase in human whole blood: Effects of different stimuli on neutrophil response to volatile anesthetic in vitro.. Int J Immunopathol Pharmacol 2017 Dec;30(4):362-370.
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