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European journal of histochemistry : EJH2010; 54(1); e2; doi: 10.4081/ejh.2010.e2

Shared Y chromosome repetitive DNA sequences in stallion and donkey as visualized using whole-genomic comparative hybridization.

Abstract: The genome of stallion (Spanish breed) and donkey (Spanish endemic Zamorano-Leonés) were compared using whole comparative genomic in situ hybridization (W-CGH) technique, with special reference to the variability observed in the Y chromosome. Results show that these diverging genomes still share some highly repetitive DNA families localized in pericentromeric regions and, in the particular case of the Y chromosome, a sub-family of highly repeated DNA sequences, greatly expanded in the donkey genome, accounts for a large part of the chromatin in the stallion Y chromosome.
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Publication Date: 2010-01-28 PubMed ID: 20353909PubMed Central: PMC3167294DOI: 10.4081/ejh.2010.e2Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research compares the genomes of a stallion and a donkey, focusing on the variability in the Y chromosome. They find that despite divergence, the genomes still share some highly repetitive DNA sequences, especially in regards to the Y chromosome.

Background of the Research

  • The researchers aimed to compare the genomes of stallions and donkeys, two animals that belong to the same equine family but have unique genetic profiles.
  • The principle technique used was Whole Comparative Genomic in situ Hybridization (W-CGH), a method that allows for large-scale genomic comparison at the chromosomal level.
  • The study paid particular attention to differences and similarities in the Y chromosome, the sex-determining chromosome in most mammalian species, between the two animals.

Findings of the Research

  • The genomes of the Spanish breed stallion and the Spanish endemic Zamorano-Leonés donkey, despite their divergence, still share several highly repetitive DNA sequences. These are sequences of nucleotides that are repeated in the genome, often playing a role in the structure and function of the chromosomes.
  • These shared sequences are primarily found in the pericentromeric regions – the part of a chromosome close to the centromere – the location where sister chromatids are held together during cell division.
  • In the case of the Y chromosome, a sub-family of highly repetitive DNA sequences that is significantly expanded in the donkey genome forms a large part of the chromatin in the stallion’s Y chromosome. Chromatin is a complex of DNA and proteins that forms chromosomes within the nucleus of eukaryotic cells.

Implication of the Findings

  • These findings shed light on the genetic similarities and differences between stallions and donkeys, despite their diverging genomes. Understanding the shared portions of a genome can help in many genetic studies including evolution, speciation, hybridization and conservation.
  • The revelation of the shared highly repetitive DNA sequences, especially on the Y chromosome, could also enrich current understanding on male infertility in equine species and contribute to further breeding and conservation practices.
  • The finding brings a more precise understanding of the structure and function of genomes, especially sex chromosomes, which can provide a foundation for future genetic studies within and across species.

Cite This Article

APA
Gosálvez J, Crespo F, Vega-Pla JL, López-Fernández C, Cortés-Gutiérrez EI, Devila-Rodriguez MI, Mezzanotte R. (2010). Shared Y chromosome repetitive DNA sequences in stallion and donkey as visualized using whole-genomic comparative hybridization. Eur J Histochem, 54(1), e2. https://doi.org/10.4081/ejh.2010.e2

Publication

European journal of histochemistry : EJH
ISSN: 2038-8306
NlmUniqueID: 9207930
Country: Italy
Language: English
Volume: 54
Issue: 1
Pages: e2
PII: e2

Researcher Affiliations

Gosálvez, J
  • Department of Biology, Genetics Unit, Universidad Autonoma de Madrid, Madrid, spain. jaime.gosalvez@uam.es
Crespo, F
    Vega-Pla, J L
      López-Fernández, C
        Cortés-Gutiérrez, E I
          Devila-Rodriguez, M I
            Mezzanotte, R

              MeSH Terms

              • Animals
              • Comparative Genomic Hybridization
              • DNA Probes
              • Equidae / genetics
              • Horses / genetics
              • In Situ Hybridization, Fluorescence
              • Male
              • Repetitive Sequences, Nucleic Acid / genetics
              • Y Chromosome / genetics

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              Citations

              This article has been cited 5 times.
              1. Casali C, Siciliani S, Zannino L, Biggiogera M. Histochemistry for nucleic acid research: 60 years in the European Journal of Histochemistry. Eur J Histochem 2022 Apr 20;66(2).
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