Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses.
Abstract: Four bacterial strains, designated ST18(T), HM244, HM250 and DI49, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S rRNA gene sequence analysis, phenotypic characteristics and levels of DNA-DNA relatedness. Their DNA G+C content ranged from 36 to 38 mol%. Lactobacillus catenaformis, Lactobacillus vitulinus and Catenibacterium mitsuokai belong to cluster XVII of the Clostridium subphylum. Strain ST18(T) was most closely related to L. catenaformis ATCC 25536(T) in the phylogenetic tree, but these strains shared only 89.9 % (1336/1486 bp) 16S rRNA gene sequence similarity. L. catenaformis, L. vitulinus and C. mitsuokai are homofermentative bacteria, whereas ST18(T) produced CO(2) from glucose. Whereas the cell-wall peptidoglycan type of L. catenaformis and L. vitulinus wasL-Lys-L-Ala(3), that of C. mitsuokai and the subgroup represented by ST18(T) was A1gamma (L-Ala-D-Glu-meso-diaminopimelic acid). On the basis of 16S rRNA gene sequence divergence of more than 10 % from L. catenaformis as well as phenotypic characteristics, strains ST18(T), HM244, HM250 and DI49 are considered to represent a novel species of a new genus belonging to the Clostridium subphylum cluster XVII, for which the name Sharpea azabuensis gen. nov., sp. nov. is proposed. The type strain of Sharpea azabuensis is ST18(T) (=JCM 14210(T) =DSM 18934(T)).
Publication Date: 2008-12-09 PubMed ID: 19060040DOI: 10.1099/ijs.0.65543-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper centers around the discovery of a new gen. nov, sp. nov. bacterium named Sharpea azabuensis. The bacterium, which is strictly anaerobic and Gram-positive, was isolated from the faeces of thoroughbred horses in Japan.
Isolation and Characteristics of Bacterial Strains
- The paper begins with the identification of four bacterial strains, ST18(T), HM244, HM250, and DI49, that were isolated in Japan from the fresh faeces of thoroughbred horses.
- These strains exhibited characteristics such as being Gram-positive, strictly anaerobic, non-spore-forming, non-motile rods that formed in chains. They were also catalase-negative.
DNA and Phylogenetic Analysis
- The strains were grouped into the same subcluster based on DNA, phenotypic characteristics, and 16S rRNA gene sequence analysis.
- The DNA G+C content across the strains ranged between 36 to 38 mol%.
- The 16S rRNA gene sequence analysis highlighted that strain ST18(T) was closest to L. catenaformis ATCC 25536(T) in the phylogenetic tree. However, the sequence similarity was only 89.9%, indicating significant divergence.
Comparison with Other Bacteria
- Lactobacillus catenaformis, Lactobacillus vitulinus, and Catenibacterium mitsuokai belong to the Clostridium subphylum cluster XVII, similar to the new strains.
- These existing bacteria are homofermentative, while the new strain ST18(T) produces CO(2) from glucose.
- The research noted differences in cell-wall peptidoglycan type among the strains. L. catenaformis and L. vitulinus exhibited the L-Lys-L-Ala(3) type while C. mitsuokai and the subgroup represented by ST18(T) had the A1gamma (L-Ala-D-Glu-meso-diaminopimelic acid) type.
New Species Proposal
- The divergence of more than 10% from L. catenaformis in the 16S rRNA gene sequence, coupled with distinct phenotypic characteristics, suggested that strains ST18(T), HM244, HM250, and DI49 represent a new species within a new genus.
- The researchers proposed the name Sharpea azabuensis gen. nov., sp. nov. for this discovery. The type strain of Sharpea azabuensis is ST18(T).
Cite This Article
APA
Morita H, Shiratori C, Murakami M, Takami H, Toh H, Kato Y, Nakajima F, Takagi M, Akita H, Masaoka T, Hattori M.
(2008).
Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses.
Int J Syst Evol Microbiol, 58(Pt 12), 2682-2686.
https://doi.org/10.1099/ijs.0.65543-0 Publication
Researcher Affiliations
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
MeSH Terms
- Anaerobiosis
- Animals
- Feces / microbiology
- Horses / microbiology
- Lactobacillaceae / classification
- Lactobacillaceae / genetics
- Lactobacillaceae / physiology
- Molecular Sequence Data
- Phylogeny
- RNA, Ribosomal, 16S / genetics
- Species Specificity
Citations
This article has been cited 15 times.- Ilina LA, Filippova VA, Brazhnik EA, Dubrovin AV, Yildirim EA, Dunyashev TP, Laptev GY, Novikova NI, Sobolev DV, Yuzhakov AA, Laishev KA. The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (Rangifer tarandus L.) from the Russian Arctic Zone: Regional and Seasonal Effects. Animals (Basel) 2021 Mar 22;11(3).
- Amin N, Seifert J. Dynamic progression of the calf's microbiome and its influence on host health. Comput Struct Biotechnol J 2021;19:989-1001.
- Kim JS, Choe H, Lee YR, Kim KM, Park DS. Intestinibaculum porci gen. nov., sp. nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine. J Microbiol 2019 May;57(5):381-387.
- Zuo G, Hao B. On monospecific genera in prokaryotic taxonomy. Synth Syst Biotechnol 2017 Sep;2(3):226-235.
- Deusch S, Camarinha-Silva A, Conrad J, Beifuss U, Rodehutscord M, Seifert J. A Structural and Functional Elucidation of the Rumen Microbiome Influenced by Various Diets and Microenvironments. Front Microbiol 2017;8:1605.
- Liu H, Hou C, Wang G, Jia H, Yu H, Zeng X, Thacker PA, Zhang G, Qiao S. Lactobacillus reuteri I5007 Modulates Intestinal Host Defense Peptide Expression in the Model of IPEC-J2 Cells and Neonatal Piglets. Nutrients 2017 May 31;9(6).
- Tanizawa Y, Fujisawa T, Kaminuma E, Nakamura Y, Arita M. DFAST and DAGA: web-based integrated genome annotation tools and resources. Biosci Microbiota Food Health 2016;35(4):173-184.
- Kamke J, Kittelmann S, Soni P, Li Y, Tavendale M, Ganesh S, Janssen PH, Shi W, Froula J, Rubin EM, Attwood GT. Rumen metagenome and metatranscriptome analyses of low methane yield sheep reveals a Sharpea-enriched microbiome characterised by lactic acid formation and utilisation. Microbiome 2016 Oct 19;4(1):56.
- Tanner SA, Chassard C, Zihler Berner A, Lacroix C. Synergistic effects of Bifidobacterium thermophilum RBL67 and selected prebiotics on inhibition of Salmonella colonization in the swine proximal colon PolyFermS model. Gut Pathog 2014;6(1):44.
- Kittelmann S, Pinares-Patiño CS, Seedorf H, Kirk MR, Ganesh S, McEwan JC, Janssen PH. Two different bacterial community types are linked with the low-methane emission trait in sheep. PLoS One 2014;9(7):e103171.
- Malmuthuge N, Griebel PJ, Guan le L. Taxonomic identification of commensal bacteria associated with the mucosa and digesta throughout the gastrointestinal tracts of preweaned calves. Appl Environ Microbiol 2014 Mar;80(6):2021-8.
- Petri RM, Schwaiger T, Penner GB, Beauchemin KA, Forster RJ, McKinnon JJ, McAllister TA. Changes in the rumen epimural bacterial diversity of beef cattle as affected by diet and induced ruminal acidosis. Appl Environ Microbiol 2013 Jun;79(12):3744-55.
- Chen CC, Pilla R, Toresson L, Sung CH, Blake AB, Correa Lopes B, Turck J, Jergens AE, Summers SC, Unterer S, Ishii PE, Giaretta PR, Tolbert MK, Suchodolski JS. Microbial Gene Profiling and Targeted Metabolomics in Fecal Samples of Dogs With Chronic Enteropathy With or Without Increased Dysbiosis Index. J Vet Intern Med 2025 Sep-Oct;39(5):e70199.
- Carmichael MN, Dycus MM, Lourenco JM, Welch CB, Davis DB, Krause TR, Rothrock MJ, Fluharty FL, Pringle TD, Callaway TR. Ruminal Microbiome Differences in Angus Steers with Differing Feed Efficiencies during the Feedlot Finishing Phase. Microorganisms 2024 Mar 7;12(3).
- Cheng X, Du X, Liang Y, Degen AA, Wu X, Ji K, Gao Q, Xin G, Cong H, Yang G. Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs. Front Microbiol 2023;14:1264840.
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