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Animals : an open access journal from MDPI2022; 12(15); doi: 10.3390/ani12151967

Shipping Temperature, Time and Media Effects on Equine Wharton’s Jelly and Adipose Tissue Derived Mesenchymal Stromal Cells Characteristics.

Abstract: To use Mesenchymal Stromal Cells (MSCs) in equine patients, isolation and expansion are performed in a laboratory. Cells are then sent back to the veterinary clinic. The main goal of storage conditions during cell transport is to preserve their biological properties and viability. The aim of this study was to evaluate the effects of storage solutions, temperature and time on the characteristics of equine adipose tissue and Wharton's jelly-derived MSCs. We compared two different storage solutions (plasma and 0.9% NaCl), two different temperatures (4 °C and room temperature) and three time frames (6, 24, 48 h). Cell viability, colony-forming units, trilineage differentiation, the expression of CD45 and CD90 antigens and adhesion potentials were evaluated. Despite the molecular characterization and differentiation potential were not influenced by storage conditions, viability, colony-forming units and adhesion potential are influenced in different way, depending on MSCs sources. Overall, this study found that, despite equine adipose tissue MSCs being usable after 24 h of storage, cells derived from Wharton's jelly need to be used within 6 h. Moreover, while for adipose cells the best conservation solutions seems to be plasma, the cell viability of Wharton's jelly MSCs declined in both saline and plasma solution, confirming their reduced resistance to conservation.
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Publication Date: 2022-08-03 PubMed ID: 35953956PubMed Central: PMC9367575DOI: 10.3390/ani12151967Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the impact of storage solutions, temperature, and duration on the quality of Mesenchymal Stromal Cells (MSCs) derived from equine fat tissue and Wharton’s jelly. The findings suggest that these cells can be effectively used within 24 hours if preserved in plasma, while those from Wharton’s jelly need to be used within 6 hours, regardless of the type of solution they are stored in.

Research Objectives and Approach

  • The study aimed to explore the effects of storage conditions on the properties and viability of Equine Mesenchymal Stromal Cells (MSCs) sourced from adipose tissue and Wharton’s jelly – a gelatinous substance in the umbilical cord.
  • Researchers compared the impact of two different storage solutions on the cells: plasma and saline (0.9% Sodium Chloride, NaCl), two temperature settings (4 degrees Celsius and room temperature), and three time durations (6, 24, and 48 hours).

MSCs Characteristics Evaluated

  • The viability of the cells was assessed to gauge the fraction of vivo (live) cells after storage.
  • The ability to form colony-forming units was evaluated. The capacity to form these units gives an indication of the number of individual cells, initially present, that can proliferate and develop into colonies.
  • The trilineage differentiation capacity was looked at to distinguish their potential to differentiate into three essential lineages: osteoblastic (bone cells), adipogenic (fat cells), and chondrogenic (cartilage cells).
  • The expressions of CD45 and CD90 antigens were assessed. The CD45 antigen is usually present on the surface of all white blood cells, and it’s absence in MSCs typically indicates their non-hematopoietic origin. On the other hand, CD90 is a marker widely found on the surface of MSCs.
  • The adhesion potential of the cells was evaluated for understanding the cells’ capability to stick onto surfaces or with each other.

Key Findings

  • The molecular characteristics and differentiation potential of these cells were found unaffected by the storage conditions.
  • However, cell viability, potential to form colony units, and adhesive capability were impacted, in different ways, based on the source of the MSCs.
  • Adipose tissue-derived MSCs maintained their usability up to 24 hours of storage, especially when preserved in plasma. However, MSCs from Wharton’s jelly need to be used within a time frame of 6 hours, irrespective of the preservation solution used.
  • The study confirmed the sensitivity of Wharton’s jelly-derived MSCs to preservation conditions, as indicated by their declining viability in both saline and plasma solutions.

Cite This Article

APA
Iacono E, Lanci A, Gugole P, Merlo B. (2022). Shipping Temperature, Time and Media Effects on Equine Wharton’s Jelly and Adipose Tissue Derived Mesenchymal Stromal Cells Characteristics. Animals (Basel), 12(15). https://doi.org/10.3390/ani12151967

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 15

Researcher Affiliations

Iacono, Eleonora
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
  • Interdepartmental Centre for Industrial Research in Health Sciences and Technologies, University of Bologna, Via Tolara di Sopra 41/E, Ozzano Emilia, 40064 Bologna, Italy.
Lanci, Aliai
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
Gugole, Penelope
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
Merlo, Barbara
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano Emilia, 40064 Bologna, Italy.
  • Interdepartmental Centre for Industrial Research in Health Sciences and Technologies, University of Bologna, Via Tolara di Sopra 41/E, Ozzano Emilia, 40064 Bologna, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 4 times.
  1. Iacono E, Merlo B. Stem Cells in Domestic Animals: Applications in Health and Production. Animals (Basel) 2022 Oct 13;12(20).
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  2. Kilbride P. Cold Chain Delivery in Cell Therapies. Adv Exp Med Biol 2025;1486:295-302.
    doi: 10.1007/978-3-031-97297-3_22pubmed: 41136848google scholar: lookup
  3. Capone A, Merlo B, Begni F, Iacono E. Equine Colostrum-Derived Mesenchymal Stromal Cells: A Potential Resource for Veterinary Regenerative Medicine. Vet Sci 2025 Jul 19;12(7).
    doi: 10.3390/vetsci12070681pubmed: 40711341google scholar: lookup
  4. Andreoli V, Berni P, Conti V, Ramoni R, Basini G, Grolli S. Mesenchymal Stromal Cells Derived from Canine Adipose Tissue: Evaluation of the Effect of Different Shipping Vehicles Used for Clinical Administration. Int J Mol Sci 2024 Mar 18;25(6).
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