Short- and long-term effect of oral administration of micellized natural vitamin E (D-α-tocopherol) on oxidative status in race horses under intense training.
Abstract: This study tested the effect of micellized vitamin E (D-α-tocopherol; 1,400 IU/d) administered 12 and 1 h orally before training for 1 d (ST-VitE) or 8 d (LT-VitE) compared with an unsupplemented control (CONTROL) on plasma α-tocopherol, thiobarbithuric acid-reactive substances (TBARS), total glutathione (GSHt), and trolox equivalent antioxidant capacity (TEAC) in 10 race horses. Different sampling times [immediately before training (BEF) and after intense training (END) or 8 h after recovery (+8h)] were investigated. Plasma α-tocopherol concentration was greater in the ST-VitE group than the CONTROL group at +8h (P < 0.05). Natural vitamin E supplementation increased plasma α-tocopherol (P < 0.001) in the LT-VitE group by approximately 1.6-fold at BEF, END, and +8h. In all groups, TBARS tended to be slightly greater (P = 0.087) immediately after training when compared with values BEF or +8h and the lowest TBARS values were observed at +8h in LT-VitE. Vitamin E supplementation did not affect the GSHt concentrations at BEF, END, or +8h. The TEAC values were modified by the vitamin E administration (P = 0.010). The greatest TEAC was found in the LT-VitE group at all sampling times and similar concentrations were reached in the ST-VitE group at +8h. The CONTROL group was not able to maintain TEAC after training (P < 0.001), indicating consumption of antioxidants (mainly vitamin E) and consequently oxidative stress because of the antioxidant system being overwhelmed by a reduced antioxidant supply. In conclusion, micellized natural vitamin E at 1,400 IU/d for 8 d efficiently increased plasma α-tocopherol concentration of race horses undergoing intense training conditions and maintained the general oxidative status.
Publication Date: 2013-01-07 PubMed ID: 23296828DOI: 10.2527/jas.2012-5125Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates the impact of micellized natural vitamin E administered orally on oxidative status in race horses under intense training, finding that this supplementation increased plasma α-tocopherol concentration efficiently and maintained good general oxidative status.
Study Overview
- The research focused on the effects of micellized vitamin E (a form of the vitamin which is made more bio-available through the process of micellization) on race horses under rigorous training conditions.
- The study evaluated the impact of a 1400 IU daily dose of micellized vitamin E, administered 12 and 1 hour(s) prior to training. Two supplementation durations were examined: one day (the ST-VitE group) and eight days (the LT-VitE group), in comparison to a control group that received no supplementation.
Evaluation & Results
- Three measurements were taken: immediately before training (marked as BEF); after intense training (marked as END); and eight hours after training recovery (marked as +8h).
- Multiple parameters were monitored, including plasma α-tocopherol (a form of vitamin E), thiobarbithuric acid-reactive substances (TBARS, markers of oxidative damage), total glutathione (a powerful antioxidant found in cells) and a measure of overall antioxidant capacity, Trolox Equivalent Antioxidant Capacity (TEAC).
- Results indicated that plasma α-tocopherol concentration was higher in the ST-VitE group than the control group at the +8h measurement. Furthermore, the LT-VitE group showed approximately a 1.6-fold increase in plasma α-tocopherol at all sampling times.
Effects on Oxidative Status
- The research found that TBARS levels tended to rise slightly after training in all groups, suggesting an increase in oxidative damage. However, the LT-VitE group had the lowest TBARS values at +8h, indicating the beneficial impact of longer-term vitamin E supplementation.
- Vitamin E supplementation was found not to significantly affect glutathione concentrations at any of the sampling times.
- However, the Trolox Equivalent Antioxidant Capacity (TEAC) values, an indicator of overall antioxidant power, increased significantly with vitamin E administration. TEAC was highest in the LT-VitE group at all sampling times, indicating a more effective antioxidant response with longer-term supplementation.
- The control group experienced a drop in TEAC after training, indicating higher oxidative stress due to insufficient antioxidant supply, mainly vitamin E.
Conclusion
- The conclusion of the research is that oral supplementation of micellized natural vitamin E at 1400 IU daily for eight days effectively increased the plasma α-tocopherol concentration of race horses under intense training.
- This study showed that such supplementation helped maintain the general oxidative status of these horses, thus countering oxidative stress associated with intense physical training.
Cite This Article
APA
Rey AI, Segura J, Arandilla E, López-Bote CJ.
(2013).
Short- and long-term effect of oral administration of micellized natural vitamin E (D-α-tocopherol) on oxidative status in race horses under intense training.
J Anim Sci, 91(3), 1277-1284.
https://doi.org/10.2527/jas.2012-5125 Publication
Researcher Affiliations
- Dpto. Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n., 28040 Madrid, Spain. anarey@vet.ucm.es
MeSH Terms
- Administration, Oral
- Animals
- Antioxidants / metabolism
- Dietary Supplements / analysis
- Female
- Glutathione / blood
- Horses / physiology
- Male
- Micelles
- Oxidative Stress / drug effects
- Physical Conditioning, Animal
- Spectrophotometry / veterinary
- Thiobarbituric Acid Reactive Substances / metabolism
- Time Factors
- alpha-Tocopherol / administration & dosage
- alpha-Tocopherol / blood
Citations
This article has been cited 6 times.- Alghuthaymi MA, Hassan AA, Kalia A, Sayed El Ahl RMH, El Hamaky AAM, Oleksak P, Kuca K, Abd-Elsalam KA. Antifungal Nano-Therapy in Veterinary Medicine: Current Status and Future Prospects. J Fungi (Basel) 2021 Jun 22;7(7).
- Nemec Svete A, Vovk T, Bohar Topolovec M, Kruljc P. Effects of Vitamin E and Coenzyme Q(10) Supplementation on Oxidative Stress Parameters in Untrained Leisure Horses Subjected to Acute Moderate Exercise. Antioxidants (Basel) 2021 Jun 3;10(6).
- Chariou PL, Ortega-Rivera OA, Steinmetz NF. Nanocarriers for the Delivery of Medical, Veterinary, and Agricultural Active Ingredients. ACS Nano 2020 Mar 24;14(3):2678-2701.
- Bai DP, Lin XY, Huang YF, Zhang XF. Theranostics Aspects of Various Nanoparticles in Veterinary Medicine. Int J Mol Sci 2018 Oct 24;19(11).
- Kusano K, Yamazaki M, Kiuchi M, Kaneko K, Koyama K. Reference range of blood biomarkers for oxidative stress in Thoroughbred racehorses (2-5 years old). J Equine Sci 2016;27(3):125-129.
- Danchuk O, Levchenko A, da Silva Mesquita R, Danchuk V, Cengiz S, Cengiz M, Grafov A. Meeting Contemporary Challenges: Development of Nanomaterials for Veterinary Medicine. Pharmaceutics 2023 Sep 15;15(9).
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