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Home / Equine Research Bank / Equine Vet J / Short-term induced hyperinsulinaemia and dexamethasone chall...
Equine veterinary journal2023; doi: 10.1111/evj.14012

Short-term induced hyperinsulinaemia and dexamethasone challenge do not affect circulating total adiponectin concentrations in insulin-sensitive ponies.

Abstract: Hypoadiponectinaemia is a risk factor for endocrinopathic laminitis, but the directionality and nature of its association with insulin dysregulation is unclear. Objective: To investigate the effects of short-term induced hyperinsulinaemia and dexamethasone challenge on circulating [total adiponectin] and whole blood expression of adiponectin (AdipoR1 and AdipoR2), insulin, and insulin-like growth factor 1 (IGF-1) receptors in insulin-sensitive ponies. Methods: In vivo experiment. Methods: Six never-laminitic, insulin-sensitive, native-breed UK ponies first underwent a dexamethasone challenge (0.08 mg/kg i.v.) with blood samples collected every 15 min over 3 h. After a 14-day washout period, hyperinsulinaemia was induced for 9 h via a euglycaemic-hyperinsulinaemic clamp (EHC), with blood samples collected every 30 min. Serum [insulin], plasma [total adiponectin], and plasma [IGF-1] were measured using validated assays and receptor gene expression was assessed via quantitative polymerase chain reaction (qPCR). Finally, whole blood was incubated with 10-1000 ng/mL dexamethasone for 3 h at 37°C to investigate its direct effects on gene expression. Results: There were no adverse effects observed during either protocol. Dexamethasone challenge did not alter circulating [insulin] or [total adiponectin] at any time-point, but significantly upregulated AdipoR1 and IGF-1R expression at 150 and 180 min. Ex vivo incubation of whole blood with dexamethasone did not alter expression of the genes examined. There was no change in [total adiponectin] or expression of the genes examined associated with EHC-induced hyperinsulinemia. Conclusions: This was a small sample size that included only native-breed ponies; total adiponectin was measured rather than high-molecular-weight adiponectin. Conclusions: Short-term induced hyperinsulinaemia and dexamethasone challenge did not affect circulating [total adiponectin] in insulin-sensitive ponies. However, dexamethasone administration was associated with upregulation of two receptors linked to adiponectin signalling, suggesting that a physiological response occurred possibly to counteract dexamethasone-associated changes in tissue insulin sensitivity.
© 2023 MARS Horsecare and The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2023-10-06 PubMed ID: 37800859DOI: 10.1111/evj.14012Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the impact of short-term induced hyperinsulinaemia and dexamethasone challenge on insulin-sensitive ponies, particularly focusing on its effects on total adiponectin concentrations. It found no significant changes in total adiponectin levels, but a noted upregulation of two receptors associated with adiponectin signalling post dexamethasone administration.

Research Objective and Methodology

  • The primary objective of this research was to examine how short-term induced hyperinsulinaemia and a dexamethasone challenge influence circulating total adiponectin concentrations and the whole blood expression of certain receptors in insulin-sensitive ponies. The focus was on adiponectin, insulin, and insulin-like growth factor 1 (IGF-1) receptors.
  • For the research, six insulin-sensitive, never-laminitic, native-breed UK ponies were under experiment. Initially, these ponies underwent a dexamethasone challenge. Post a 14-day recovery period, hyperinsulinaemia was induced.
  • Serum insulin, plasma total adiponectin, and plasma IGF-1 concentrations were measured using validated assays, while receptor gene expression was evaluated through quantitative polymerase chain reaction (qPCR).
  • The effect of dexamethasone on gene expression was also investigated by incubating whole blood with the substance.

Research Findings

  • There were no adverse effects observed during the procedure, confirming the safety of both protocols.
  • The dexamethasone challenge did not cause any alteration in circulating insulin or total adiponectin levels at any measured time-point. Fluctuations were only seen in the upregulation of AdipoR1 and IGF-1R expression at 150 and 180 minutes.
  • There was no impact on total adiponectin levels or the expression of the examined genes due to hyperinsulinemia induced by EHC – euglycaemic-hyperinsulinaemic clamp.
  • Ex vivo dexamethasone incubation of whole blood showed no impact on the genes’ expression.

Conclusions

  • Despite the small sample size and focus on native-breed ponies only, the study provided some insights about the short-term induced hyperinsulinaemia and dexamethasone challenge on insulin-sensitive ponies.
  • No impact on circulating total adiponectin levels was seen. However, dexamethasone administration coincided with the upregulation of two receptor links to adiponectin signalling, suggesting a potential physiological response that could counter dexamethasone-associated changes in tissue insulin sensitivity.

Cite This Article

APA
Barnabé MA, Elliott J, Harris PA, Menzies-Gow NJ. (2023). Short-term induced hyperinsulinaemia and dexamethasone challenge do not affect circulating total adiponectin concentrations in insulin-sensitive ponies. Equine Vet J. https://doi.org/10.1111/evj.14012

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English

Researcher Affiliations

Barnabé, Marine A
  • Department of Clinical Sciences and Services, Royal Veterinary College, Hertfordshire, UK.
Elliott, Jonathan
  • Department of Comparative Biomedical Sciences, Royal Veterinary College, Hertfordshire, UK.
Harris, Patricia A
  • Equine Studies Group, Waltham Petcare Science Institute, Leicestershire, UK.
Menzies-Gow, Nicola J
  • Department of Clinical Sciences and Services, Royal Veterinary College, Hertfordshire, UK.

Grant Funding

  • Royal Veterinary College Mellon Fund
  • WALTHAM Petcare Science Institute

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