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The Canadian veterinary journal = La revue veterinaire canadienne2013; 53(12); 1266-1272;

Simple tube centrifugation for processing platelet-rich plasma in the horse.

Abstract: This study evaluated the quality and bacteriologic safety of platelet-rich plasma (PRP) produced by 3 simple, inexpensive tube centrifugation methods and a commercial system. Citrated equine blood collected from 26 normal horses was processed by 4 methods: blood collection tubes centrifuged at 1200 and 2000 × g, 50-mL conical tube, and a commercial system. White blood cell (WBC), red blood cell (RBC), and platelet counts and mean platelet volume (MPV) were determined for whole blood and PRP, and aerobic and anaerobic cultures were performed. Mean platelet concentrations ranged from 1.55- to 2.58-fold. The conical method yielded the most samples with platelet concentrations greater than 2.5-fold and within the clinically acceptable range of > 250,000 platelets/μL. White blood cell counts were lowest with the commercial system and unacceptably high with the blood collection tubes. The conical tube method may offer an economically feasible and comparatively safe alternative to commercial PRP production systems. Centrifugation en tube pour le traitement du plasma riche en plaquettes chez le cheval. Cette étude a évalué la qualité et l’innocuité bactériologique du plasma riche en plaquettes (PRP) produit par 3 méthodes simples et économiques de centrifugation en tube et un système commercial. Du sang équin citraté prélevé de 26 chevaux normaux a été traité à l’aide de 4 méthodes : des tubes de prélèvement du sang centrifugés à 1200 et à 2000 × g, un tube conique de 50 ml et un système commercial. La numération des globules blancs, des globules rouges et des plaquettes ainsi que le volume moyen des plaquettes ont été déterminés pour le sang total et le PRP et des cultures bactériennes en aérobiose et en anaérobiose ont été réalisées. Les concentrations moyennes des plaquettes s’échelonnaient d’un ordre de grandeur variant de 1,55 à 2,58. La méthode conique a donné le plus d’échantillons avec des concentrations de plaquettes supérieures à un ordre de 2,5 et dans la fourchette cliniquement acceptable de > 250 000 plaquettes/μL. La numération des globules blancs était la plus basse avec le système commercial et était trop élevée par la méthode avec les tubes de prélèvement du sang. La méthode du tube conique peut offrir une méthode de remplacement abordable et d’innocuité comparable aux systèmes de production commerciaux de PRP.(Traduit par Isabelle Vallières).
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Publication Date: 2013-06-05 PubMed ID: 23729823PubMed Central: PMC3500116
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  • Journal Article
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study assessed the quality and safety of platelet-rich plasma (PRP) produced from horse blood using different centrifugation techniques and found that the simpler, low-cost conical tube method can serve as an economically-feasible and comparably safe alternative to commercial systems for PRP production.

Research Methodology

  • The researchers conducted an experiment on citrated equine blood collected from 26 healthy horses.
  • The blood was processed using four different methods: blood collection tubes centrifuged at 1200 and 2000 × g, a 50-mL conical tube, and a commercial system.
  • The researchers evaluated the resultant platelet-rich plasma (PRP) for its quality and safety. They determined the red blood cell (RBC), white blood cell (WBC), and platelet counts and the mean platelet volume (MPV) for both the whole blood and the PRP obtained.
  • Bacteriologic safety was tested by performing aerobic and anaerobic cultures.

Research Findings

  • The average concentrations of platelets in the PRP samples ranged between 1.55 and 2.58-fold.
  • The conical method consistently produced PRP samples with platelet concentrations greater than 2.5-fold and within the clinically acceptable range of over 250,000 platelets/μL.
  • The commercial method achieved the lowest white blood cell counts, whereas the blood collection tubes method produced unacceptably high counts.

Conclusions

  • The study found the conical tube method to be potentially an economically feasible and comparatively safe alternative to the commercially available systems for PRP production.
  • This simplicity and cost-effectiveness can be important for veterinary medicine, providing an accessible treatment option.

Recommendations

  • Further research may be needed to optimize the procedure and further test for its safety and effectiveness across different scenarios.

Cite This Article

APA
Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. (2013). Simple tube centrifugation for processing platelet-rich plasma in the horse. Can Vet J, 53(12), 1266-1272.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 53
Issue: 12
Pages: 1266-1272

Researcher Affiliations

Fontenot, Robin L
  • Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0442, USA.
Sink, Carolyn A
    Werre, Stephen R
      Weinstein, Nicole M
        Dahlgren, Linda A

          MeSH Terms

          • Animals
          • Centrifugation / methods
          • Centrifugation / veterinary
          • Erythrocyte Count / methods
          • Erythrocyte Count / veterinary
          • Female
          • Horse Diseases / blood
          • Horse Diseases / therapy
          • Horses / blood
          • Leukocyte Count / methods
          • Leukocyte Count / veterinary
          • Male
          • Platelet Count / methods
          • Platelet Count / veterinary
          • Platelet-Rich Plasma / cytology

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          Citations

          This article has been cited 12 times.
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