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Reproduction in domestic animals = Zuchthygiene2020; 55(3); 374-383; doi: 10.1111/rda.13632

Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG.

Abstract: The aim of this study was to assess the efficacy of different doses of buserelin acetate and another GnRH agonist, triptorelin acetate, in saline solution in a single subcutaneous injection, to induce ovulation of growing pre-ovulatory follicle in mare and compare it with the classical treatment of a single injection of hCG. The study is split into 3 experiments over different breeding seasons in the same stud with a random distribution of treatment. The first one was to compare the injection of 6 mg of buserelin with 1,500 IU of hCG; the second one consisted of comparing different doses of buserelin (6 mg and 3 mg); and the third one compared three different doses of buserelin (3, 2 and 1 mg), 0.1 mg of triptorelin with 1,500 IU of hCG as a control group. The results of all experiments showed the same efficacy between all treatments with mares ovulating between 24 and 48 hr after injection: experiment 1: hCG (78% n = 41) and buserelin 6 mg (90% n = 50); experiment 2: buserelin 6 mg (78,1% n = 192) and buserelin 3 mg (78% n = 341); and experiment 3: hCG (87% n = 106), buserelin 3 mg (84,7% n = 137), buserelin 2 mg (82,7% n = 104), buserelin 1 mg (87% n = 54) and triptorelin 0.1 mg (84,7% n = 72). In conclusion, this study contributes to erasing the dogma that has been established since 1975 that a single injection in solution without any long-acting excipient of a GnRH agonist cannot induce ovulation in the mare. This study also shows that a injection of 0.1 mg of triptorelin in solution is a good alternative for ovulation induction and is comparable to small doses of buserelin acetate in solution (1 mg) and 1,500 IU of the gold standard trigger hCG, mainly in countries where human formulation of buserelin is not available.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-01-31 PubMed ID: 31930759DOI: 10.1111/rda.13632Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the effectiveness of different doses of the hormones buserelin and triptorelin, compared to a standard injection of hCG, in inducing ovulation in mares. The experiments show that a simple injection of buserelin or triptorelin leads to similar results to the traditional hCG treatment.

Research Objectives and Methodology

  • The primary aim of this study was to evaluate the effectiveness of different doses of the hormones buserelin acetate and triptorelin acetate, compared with the standard injection of hCG, in triggering ovulation in mares.
  • The researchers divided the study into three separate experiments, each conducted in different breeding seasons at the same stud farm with randomly designated treatment strategies.
  • The first experiment compared the impact of a 6 mg buserelin injection to a 1,500 IU hCG injection.
  • The second phase analyzed the effects of different doses of buserelin (3 mg and 6 mg).
  • The third experiment compared three different doses of buserelin (1 mg, 2 mg, and 3 mg), with 0.1 mg of triptorelin, and 1,500 IU hCG as the control group.

Research Findings

  • The results from all three experiments demonstrated similar efficacy among all the treatments, with the mares typically ovulating between 24 and 48 hours after injection.
  • In the first experiment, the ovulation rate was 78% with hCG and 90% with 6 mg of buserelin.
  • In the second experiment, 78.1% of mares ovulated with a 6 mg dose of buserelin, and 78% with a 3 mg dose.
  • For the third experiment, hCG had an 87% ovulation rate. Varying doses of buserelin resulted in ovulation rates of 84.7% (3 mg), 82.7% (2 mg), and 87% (1 mg), and the 0.1 mg dose of triptorelin resulted in an ovulation rate of 84.7%.

Conclusion and Implications

  • The findings of this study challenge the widely held belief since 1975 that a single injection without a long-term excipient of a GnRH agonist cannot trigger ovulation in mares.
  • The study also demonstrates that a single 0.1 mg injection of triptorelin can be an effective alternative to smaller doses of buserelin acetate and the “gold standard” hCG, particularly in regions where human buserelin is not readily available.

Cite This Article

APA
Dordas-Perpinyà M, Normandin L, Dhier T, Terris H, Cochard A, Frilley C, Huiban F, Bruyas JF. (2020). Single injection of triptorelin or buserelin acetate in saline solution induces ovulation in mares the same as a single injection of hCG. Reprod Domest Anim, 55(3), 374-383. https://doi.org/10.1111/rda.13632

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 3
Pages: 374-383

Researcher Affiliations

Dordas-Perpinyà, Marta
  • Theriogenology unit, Nantes Veterinary College, ONIRIS BP 40706, Nantes cedex, France.
Normandin, Laure
  • Haras de Hus, Petit Mars, France.
Dhier, Thibault
  • Haras de Hus, Petit Mars, France.
Terris, Hubert
  • Haras de Hus, Petit Mars, France.
Cochard, Anaïs
  • Theriogenology unit, Nantes Veterinary College, ONIRIS BP 40706, Nantes cedex, France.
Frilley, Camille
  • Theriogenology unit, Nantes Veterinary College, ONIRIS BP 40706, Nantes cedex, France.
Huiban, Fanny
  • Theriogenology unit, Nantes Veterinary College, ONIRIS BP 40706, Nantes cedex, France.
Bruyas, Jean-François
  • Theriogenology unit, Nantes Veterinary College, ONIRIS BP 40706, Nantes cedex, France.

MeSH Terms

  • Animals
  • Breeding
  • Buserelin / administration & dosage
  • Buserelin / pharmacology
  • Chorionic Gonadotropin / administration & dosage
  • Chorionic Gonadotropin / pharmacology
  • Female
  • Fertility Agents, Female / administration & dosage
  • Fertility Agents, Female / pharmacology
  • Horses / physiology
  • Injections, Subcutaneous / veterinary
  • Ovulation Induction / methods
  • Ovulation Induction / veterinary
  • Triptorelin Pamoate / administration & dosage
  • Triptorelin Pamoate / pharmacology

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Citations

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