Single Nucleotide Polymorphism in Interleukin-6 and Interleukin-8 Genes of Equines.
Abstract: In equines, muscle exercise highly affects the expression of genes which are associated with secretion of cytokines. Myokines are the group of cytokines that are produced by skeletal muscles during exercise. Interleukin-6 (IL-6) and Interleukin-8 (IL-8) are among such myokines. This study was therefore taken up in 42 animals of Marwari, Kathiawari, Manipuri and Zanskari horses; and Poitou and Halari donkeys to find out the Single Nucleotide Polymorphisms (SNPs) in the IL-6 and IL-8 genes. Sequence analysis of exon 2 of IL-6 gene revealed an intronic SNP (C>T) at position Equcab3.0:4:54607583 in Poitou donkey. No SNP was detected in the equines covered in the study for IL-6 exon 5, exon 6 and their flanking sequences. However, in exon 1 of the IL-8 gene of all 4 horse breeds and 2 donkey breeds, a non-synonymous SNP (G > C) leading to a change in amino acid from leucine (L) to phenylalanine (F) (p. Leu10> p. Phe10) was observed at position EquCab3.0:3:63720817. Another nucleotide substitution was T>C in the intron 1-2 of IL-8 gene in all equids studied at position EquCab3.0:3:63720770. In addition to above two substitutions, in the 5' UTR of IL-8 gene in Poitou donkey, in place of guanosine (G), heterozygous condition S (C/G) was observed at position EquCab3.0:3: 63720883. This paper presents four SNPs with one non-synonymous SNP leading to a change in IL-8 protein sequence in equines.
Copyright © 2022. Published by Elsevier Inc.
Publication Date: 2022-06-28 PubMed ID: 35777577DOI: 10.1016/j.jevs.2022.104058Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers investigated the single nucleotide polymorphism (SNPs) of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) genes in different breeds of horses and donkeys. They discovered four SNPs, including one non-synonymous SNP which led to a change in the IL-8 protein sequence.
Study Overview
This study aimed to discover Single Nucleotide Polymorphisms (SNPs) in the IL-6 and IL-8 genes of various horse and donkey breeds.
- Subjects: The study was conducted using 42 animals from Marwari, Kathiawari, Manipuri, and Zanskari horse breeds, and Poitou and Halari donkey breeds.
- Focus of Investigation: Researchers focused on the expression of genes associated with the production of cytokines, specifically IL-6 and IL-8, which are produced by skeletal muscles during exercise.
Findings
The investigation revealed SNPs in both the IL-6 and IL-8 genes with varying occurrence among the different breeds of equines studied.
- IL-6 Gene: Sequence analysis of exon 2 of the IL-6 gene revealed an intronic SNP (C>T) at position Equcab3.0:4:54607583 in Poitou donkey. However, no SNP was detected in the IL-6 exon 5, exon 6 and their flanking sequences for any of the equines covered.
- IL-8 Gene: In exon 1 of the IL-8 gene of all the breeds, a non-synonymous SNP (G > C) which led to a change in amino acid from leucine (L) to phenylalanine (F) (p. Leu10 > p. Phe10) was observed at position EquCab3.0:3:63720817. Another nucleotide substitution (T>C) was found in the intron 1-2 of IL-8 gene in all equids studied at position EquCab3.0:3:63720770. A third change was found in the 5′ UTR of IL-8 gene in Poitou donkey, where the guanosine (G) was changed to a heterozygous condition S (C/G) at position EquCab3.0:3: 63720883.
Conclusion
In total, the research paper presents four SNPs with one non-synonymous SNP leading to a change in the IL-8 protein’s sequence in equines. These findings add to our understanding of genetic variation in equines related to cytokine production, particularly in response to muscle exercise.
Cite This Article
APA
Kumar S, Ashraf M, Pannu U, Mehta SC.
(2022).
Single Nucleotide Polymorphism in Interleukin-6 and Interleukin-8 Genes of Equines.
J Equine Vet Sci, 117, 104058.
https://doi.org/10.1016/j.jevs.2022.104058 Publication
Researcher Affiliations
- College of Veterinary and Animal Science, RAJUVAS, Bikaner, India.
- College of Veterinary and Animal Science, RAJUVAS, Bikaner, India.
- College of Veterinary and Animal Science, RAJUVAS, Bikaner, India.
- Principal Scientist, ICAR-National Research Center on Equines, Equine Production Campus, Bikaner, India. Electronic address: scmehta64@gmail.com.
MeSH Terms
- 5' Untranslated Regions
- Animals
- Equidae / genetics
- Guanosine
- Horses / genetics
- Interleukin-6 / genetics
- Interleukin-8 / genetics
- Leucine / genetics
- Nucleotides
- Phenylalanine / genetics
- Physical Conditioning, Animal
- Polymorphism, Single Nucleotide / genetics
Citations
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