Single nucleotide polymorphisms of interleukin-1 beta related genes and their associations with infection in the horse.
Abstract: In previous work, we found significant associations of horse chromosome 15 (ECA15) microsatellite markers HMSO1 and HTG06 with two horse infections, Rhodococcus equi and Lawsonia intracellularis, respectively. Interleukin-1 beta subunit and interleukin-1 receptor antagonist encoding genes (IL1B and IL1RN) could be considered as candidate genes underlying the associations reported. Therefore, we identified single nucleotide polymorphisms (SNPs) within three interleukin-1 beta functionally related genes: IL1B, IL1RN and Casp1 (interleukin-1 beta converting enzyme/caspasel encoding gene). Using appropriate restriction fragment length polymorphism (PCR-RFLP) and/or single strand conformation polymorphism (PCR-SSCP) markers, their associations with the two infections by genotyping foals from the original study were tested. In addition, the physical localization of one of the two closely located genes, IL1RN, was re-assessed by fluorescence in-situ hybridization (FISH). A statistically significant association between an intronic SNP of the Casp1 gene with R. equi infection was found. The IL1RN gene was localized to 15q13-q14 in agreement with its originally reported physical position.
Publication Date: 2008-09-27 PubMed ID: 18817325DOI: 10.1159/000317184Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the identification of specific Single Nucleotide Polymorphisms (SNPs) in genes related to interleukin-1-beta and their connection to the infectious diseases Rhodococcus equi and Lawsonia intracellularis in horses. It found a statistically significant association between a SNP in the Casp1 gene and R. equi infection in horses.
Identification of Single Nucleotide Polymorphisms
- The authors first discovered the connection between certain horse infections, specifically Rhodococcus equi and Lawsonia intracellularis, and marker genes on horse chromosome 15 (ECA15).
- Their findings led them to consider genes IL1B and IL1RN, encoding for interleukin-1 beta subunit and interleukin-1 receptor antagonist respectively, as potential candidates behind these associations.
- In this study, they identified SNPs within three genes that are functionally related to interleukin-1-beta: IL1B, IL1RN, and Casp1, also known as the caspase-1 encoding gene.
Association with Infections
- The identified SNPs were tested for their association with the aforementioned infections. This was done by genotyping foals from the original study using PCR-RFLP (Restriction Fragment Length Polymorphism) and/or PCR-SSCP (Single Strand Conformation Polymorphism) markers.
- The result of this testing showed a statistically significant association between an intronic SNP of the Casp1 gene and R. equi infection.
Physical Localization of Genes
- The study also reassessed the physical localization of the IL1RN gene, one of the two genes closely located, using fluorescence in-situ hybridization (FISH) technique.
- The FISH method affirmed the original location of the IL1RN gene at 15q13-q14 on horse chromosome 15, consistent with the previously reported physical position.
Conclusions
- This research adds important knowledge to description of the genetic background of equine health, specifically the relationship between specific SNPs and susceptibility to certain infections.
- By discovering the SNP within the Casp1 gene and its association with R. equi infection, there is a potential to use this information for predicting and mitigating the disease
- Finding the connection between IL1-related gene SNPs and horse infections could further lead to therapeutic strategies to address these infectious diseases.
Cite This Article
APA
Horin P, Osickova J, Necesankova M, Matiasovic J, Musilova P, Kubickova S, Hubertova D, Vyskocil M, Rubes J.
(2008).
Single nucleotide polymorphisms of interleukin-1 beta related genes and their associations with infection in the horse.
Dev Biol (Basel), 132, 347-351.
https://doi.org/10.1159/000317184 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Base Sequence
- Chromosome Mapping / veterinary
- DNA Primers
- Horse Diseases / genetics
- Horses / genetics
- Interleukin-1beta / genetics
- Polymorphism, Single Nucleotide
Citations
This article has been cited 3 times.- Maddahi N, Sadeghi M, Sarghale AJ, Saatchi M, Davar Siar MK, Kholghi M. Identification of candidate gene networks affecting the number of somatic cells count and milk production in Iranian Holstein cows using Genome-wide association study. Sci Rep 2025 Sep 1;15(1):32168.
- Alim MA, Dong T, Xie Y, Wu XP, Zhang Y, Zhang S, Sun DX. Effect of polymorphisms in the CSN3 (κ-casein) gene on milk production traits in Chinese Holstein Cattle. Mol Biol Rep 2014 Nov;41(11):7585-93.
- Jiang L, Liu J, Sun D, Ma P, Ding X, Yu Y, Zhang Q. Genome wide association studies for milk production traits in Chinese Holstein population. PLoS One 2010 Oct 27;5(10):e13661.
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