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Small interfering RNA targeting bovine papillomavirus type 1 E2 induces apoptosis in equine sarcoid transformed fibroblasts.
Abstract: Equine sarcoids are skin tumours of horses caused by infection with BPV-1 or 2. Maintenance and replication of the viral genome depend upon the viral proteins E1 and E2. We examined the effects of an E2 specific siRNA on E2 and E1 viral gene expression, viral load and cell growth in BPV-1 transformed sarcoid-derived cells. Transfection with E2-siRNA caused a reduction in E2 and E1 mRNA expression as well as viral load, growth inhibition and decreased anchorage-independent growth. siRNA treated cells showed significantly higher apoptosis rates than control cells. Thus sequence specific targeting of E2 provides a powerful strategy to eliminate BPV-1 genomes and induce cell death in BPV-1 transformed cells.
Publication Date: 2009-07-15 PubMed ID: 19615418DOI: 10.1016/j.virusres.2009.06.019Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article investigates the effects of a specific small interfering RNA (siRNA) on the bovine papillomavirus type 1 (BPV-1), which causes equine sarcoids, a type of skin tumour in horses. The study finds that this type of siRNA can reduce viral load, inhibit cell growth, and induce cell death in BPV-1 infected cells.
Research Objective and Methodology
- The research aimed to study how an E2-specific siRNA affects the E1 and E2 viral gene expressions, viral load, and cell growth in BPV-1 transformed sarcoid cells.
- This study involved infecting cells derived from equine sarcoids with BPV-1. These cells were then treated with E2-specific siRNA.
- The effects of this treatment on the infected cells’ viral gene expression, viral load, and cell growth were then examined.
Key Findings
- The transfection of the cells with the E2-specific siRNA resulted in a decrease in the expression of both E1 and E2 mRNA.
- Similarly, viral load in the treated cells was lower than in untreated ones, implying the siRNA treatment was successful in reducing the amount of the virus in the cells.
- The growth of the affected cells was also inhibited, and there was a reduction in their ability to grow independently from a surface (anchorage-independent growth).
- Furthermore, the treated cells exhibited significantly higher levels of apoptosis (regulated cell death) compared to control cells.
Conclusion and Implications
- Based on these results, the research concludes that sequence-specific targeting of E2, done through the use of a specific siRNA, offers an effective strategy to both eliminate BPV-1 genomes and induce cell death in BPV-1-transformed cells.
- This study has implications for the treatment of BPV-1 or BPV-2 infections, potentially reducing the prevalence of equine sarcoids in horses and improving their health and lifespan.
- Further work needs to be done to field test this therapeutic strategy and determine its broad applicability and any potential side effects.
Cite This Article
APA
Gobeil PA, Yuan Z, Gault EA, Morgan IM, Campo MS, Nasir L.
(2009).
Small interfering RNA targeting bovine papillomavirus type 1 E2 induces apoptosis in equine sarcoid transformed fibroblasts.
Virus Res, 145(1), 162-165.
https://doi.org/10.1016/j.virusres.2009.06.019 Publication
Researcher Affiliations
- Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Garscube Campus, Scotland, UK.
MeSH Terms
- Animals
- Apoptosis
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / metabolism
- Cell Line, Tumor
- DNA-Binding Proteins / genetics
- DNA-Binding Proteins / metabolism
- Fibroblasts / physiology
- Fibroblasts / virology
- Gene Expression
- Genetic Therapy
- Genome, Viral
- Horses
- Papillomavirus Infections / metabolism
- Papillomavirus Infections / therapy
- Papillomavirus Infections / virology
- RNA Interference
- RNA, Small Interfering / genetics
- RNA, Small Interfering / metabolism
- RNA, Viral / genetics
- RNA, Viral / metabolism
- Viral Proteins / genetics
- Viral Proteins / metabolism
Citations
This article has been cited 3 times.- Tsai MM, Wang CS, Tsai CY, Huang HW, Chi HC, Lin YH, Lu PH, Lin KH. Potential Diagnostic, Prognostic and Therapeutic Targets of MicroRNAs in Human Gastric Cancer. Int J Mol Sci 2016 Jun 16;17(6).
- Finlay M, Yuan Z, Morgan IM, Campo MS, Nasir L. Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and show aberrant expression of p53. Vet Res 2012 Dec 4;43(1):81.
- Monod A, Koch C, Jindra C, Haspeslagh M, Howald D, Wenker C, Gerber V, Rottenberg S, Hahn K. CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts. Viruses 2023 Sep 17;15(9).
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