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Home / Equine Research Bank / Lab Chip / Smartphone-based multiplex 30-minute nucleic acid test of li...
Lab on a chip2020; 20(9); 1621-1627; doi: 10.1039/d0lc00304b

Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.

Abstract: Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
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Publication Date: 2020-04-26 PubMed ID: 32334422DOI: 10.1039/d0lc00304bGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a smartphone-based, fast, and efficient method for “point-of-care” detection of live virus particles specific to respiratory infections, similar to COVID-19, using horse samples as models.

Summary of Research

  • Authored by a group of researchers, this study sets out a method for the rapid, sensitive, and specific identification of infectious pathogens which has major implications for patient management and epidemic surveillance. The proposed system, which can be integrated with a smartphone, allows for the detection of live virus from nasal swab media.
  • The research team used a panel of equine (horse) respiratory infectious diseases as a model system. This was done due to the degree of correlation these diseases have with human diseases such as COVID-19.

Nucleic Acid Detection

  • According to the research, specific nucleic acid sequences of five pathogens were amplified by a process known as ‘loop-mediated isothermal amplification’ on a microfluidic chip. This basically means that the nucleic acid sequences of these pathogens were duplicated many times over, which makes it easier to detect them.
  • The detection process takes place at the end of the chemical reactions. This amplification and detection process is significantly faster with results being available in approximately 30 minutes, which is considerably quicker than traditional lab-based diagnostic tests.

Testing and Results

  • To validate their system, the researchers used pathogen-spiked horse nasal swab samples. These samples were correctly diagnosed with the smartphone-based system and demonstrated its effectiveness in identifying the presence of a pathogen.
  • The limit of detection – the smallest amount of pathogen that could be reliably detected – was found to be comparable to that of the traditional lab-based test, polymerase chain reaction (PCR). This means that this newer, faster testing system is as sensitive as the traditional, slower test.

Implications of the Research

  • The research demonstrates the capabilities of a sensitive, portable, and quick method for on-site virus detection using a smartphone-integrated system. The results can be available in substantially less time when compared to conventional laboratory diagnosis methods.
  • While the detection targeted equine diseases in this study, the same approach could be applied to human diseases such as COVID-19, thus having a significant impact on epidemic surveillance and patient care techniques.

Cite This Article

APA
Sun F, Ganguli A, Nguyen J, Brisbin R, Shanmugam K, Hirschberg DL, Wheeler MB, Bashir R, Nash DM, Cunningham BT. (2020). Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract. Lab Chip, 20(9), 1621-1627. https://doi.org/10.1039/d0lc00304b

Publication

Lab on a chip
ISSN: 1473-0189
NlmUniqueID: 101128948
Country: England
Language: English
Volume: 20
Issue: 9
Pages: 1621-1627

Researcher Affiliations

Sun, Fu
  • Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu.
Ganguli, Anurup
  • Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Nguyen, Judy
  • RAIN Incubator, Tacoma, Washington, USA.
Brisbin, Ryan
  • Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
Shanmugam, Krithika
  • Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
Hirschberg, David L
  • RAIN Incubator, Tacoma, Washington, USA and Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
Wheeler, Matthew B
  • Department of Animal Sciences, University of Illinois at Urbana-Champaign, Illinois, USA.
Bashir, Rashid
  • Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Nash, David M
  • Private equine veterinarian, Kentucky, USA.
Cunningham, Brian T
  • Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.

MeSH Terms

  • Animals
  • Betacoronavirus / isolation & purification
  • COVID-19 Testing
  • Clinical Laboratory Techniques / methods
  • Coronavirus Infections / diagnosis
  • Herpesvirus 1, Equid / isolation & purification
  • Herpesvirus 4, Equid / isolation & purification
  • Horse Diseases / diagnosis
  • Horse Diseases / microbiology
  • Horse Diseases / virology
  • Horses
  • Influenza A Virus, H3N8 Subtype / isolation & purification
  • Lab-On-A-Chip Devices
  • Mobile Applications
  • Molecular Diagnostic Techniques / methods
  • Nose / microbiology
  • Nose / virology
  • Nucleic Acid Amplification Techniques / methods
  • Point-of-Care Systems
  • Respiration Disorders / diagnosis
  • Respiration Disorders / microbiology
  • Respiration Disorders / veterinary
  • Respiration Disorders / virology
  • SARS-CoV-2
  • Smartphone
  • Streptococcus equi / isolation & purification

Grant Funding

  • 1534126 / National Science Foundation

Citations

This article has been cited 52 times.
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