Some properties of different skeletal muscle fiber types: comparison of reference bases.
Abstract: Several biochemical components of the white portion of the gastrocnemius (WGM), plantaris (PM), and soleus (SM) muscles of the rat and middle gluteal (MGM) muscle of the horse were compared based on wet and dry weight, protein, and total creatine concentrations ([TCr]). The water content was similar for the rat hindlimb muscles, however, the concentrations of protein, ATP, phosphocreatine (PCr), creatine, and glycogen ranked as SM less than PM less than WGM for all reference bases except total creatine. In contrast, concentrations of ATP, creatine, and PCr were similar in all muscles studied when expressed as ratios of [TCr]. Horse MGM had the lowest percent of water and protein per gram wet or dry weight but highest glycogen concentration of the muscles studied, irrespective of the reference base used to express concentrations. Coefficients of variation were lowest when muscle constituents were related to [TCr]. It is concluded that expressing muscle constituents relative to total creatine results in the smallest variation and is a good method for making comparisons between muscles of similar fiber composition. However, essential information concerning different types of muscle may be lost when this reference base is used.
Publication Date: 1987-04-01 PubMed ID: 3036755DOI: 10.1152/jappl.1987.62.4.1436Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on comparing the biochemical components of different types of skeletal muscle fibers in rats and horses, highlighting that using total creatine as a reference basis for comparison minimizes variation but may also obscure informative differences between muscle types.
Introduction to the Study
- The study undertaken by the researchers investigates the biochemical properties of different types of skeletal muscle fibers, using muscles from rats and horses to explore these comparisons.
- These muscles include the white portion of the gastrocnemius (WGM), plantaris (PM), and soleus (SM) muscles in rats, and the middle gluteal (MGM) muscle in horses.
- The biochemical components in focus are the following: wet and dry weight, protein, total creatine concentrations ([TCr]), water content, ATP, phosphocreatine (PCr), creatine, and glycogen.
Findings
- The water content was found to be similar across the rat hindlimb muscles (WGM, PM, and SM). However, concentrations of protein, ATP, PCr, creatine, and glycogen differed, ranking from lowest to highest in the order of SM < PM < WGM for all reference bases, except for total creatine.
- Contrasting the earlier conclusion, the concentrations of ATP, creatine, and PCr were found to be similar in all muscles when expressed as ratios of [TCr].
- The MGM muscle in horses had the lowest percentage of water and protein per gram of wet or dry weight, but the highest glycogen concentration among the muscles studied, irrespective of the reference base used.
Conclusion and Implications
- It was concluded that when muscle constituents were related to total creatine ([TCr]), the coefficients of variation were lowest, suggesting that using total creatine as a reference base is a robust method for comparing muscles with similar fiber compositions.
- Despite this fact, the researchers noted that essential information about different types of muscles might often be overlooked when this reference base (total creatine) is used, hinting at the potential need for multiple comparison methods or bases to truly account for the nuanced differences between muscle types.
Cite This Article
APA
Kelso TB, Hodgson DR, Visscher AR, Gollnick PD.
(1987).
Some properties of different skeletal muscle fiber types: comparison of reference bases.
J Appl Physiol (1985), 62(4), 1436-1441.
https://doi.org/10.1152/jappl.1987.62.4.1436 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Body Water / metabolism
- Creatine / metabolism
- Glycogen / metabolism
- Horses
- Male
- Muscle Proteins / metabolism
- Muscles / metabolism
- Osmolar Concentration
- Perchlorates
- Rats
- Rats, Inbred Strains
- Reference Values
Citations
This article has been cited 5 times.- Snow RJ, Murphy RM. Creatine and the creatine transporter: a review.. Mol Cell Biochem 2001 Aug;224(1-2):169-81.
- Chen J, Gollnick PD. Effect of exercise on hexokinase distribution and mitochondrial respiration in skeletal muscle.. Pflugers Arch 1994 Jun;427(3-4):257-63.
- Gollnick PD, Bertocci LA, Kelso TB, Witt EH, Hodgson DR. The effect of high-intensity exercise on the respiratory capacity of skeletal muscle.. Pflugers Arch 1990 Jan;415(4):407-13.
- Hodgson DR, Rose RJ, Kelso TB, McCutcheon LJ, Bayly WM, Gollnick PD. Respiratory and metabolic responses in the horse during moderate and heavy exercise.. Pflugers Arch 1990 Sep;417(1):73-8.
- Tate CA, Hyek MF, Taffet GE. The role of calcium in the energetics of contracting skeletal muscle.. Sports Med 1991 Sep;12(3):208-17.
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