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Scientific reports2019; 9(1); 15072; doi: 10.1038/s41598-019-51413-4

Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation.

Abstract: This study aimed to determine the effect of bull seminal plasma (SP) and sperm on endometrial function. Bovine endometrial explants were incubated with: ejaculated sperm with or without SP, epididymal sperm, or SP alone. Neither ejaculated nor epididymal sperm induced differential expression of IL1A, IL1B, IL6, IL8, PTGES2, TNFA, and LIF. Interestingly, SP had a detrimental effect on endometrial RNA integrity. Addition of an RNase inactivation reagent to SP blocked this effect, evidencing a role for a SP-RNase. Because bulls deposit the ejaculate in the vagina, we hypothesized that the bovine endometrium is more sensitive to SP-RNase than vaginal and cervical tissues (which come into contact with SP during mating), or to endometrium from intrauterine ejaculators (such as the horse). In addition, due to differences in SP-RNase abundance depending on SP collection method (i.e., with an artificial vagina, AV, or by electroejaculation, EE), this effect was also tested. Bull SP, collected by AV, degrades RNA of mare endometrium, and bovine vagina, cervix and endometrium. However, stallion SP or bull SP collected by EE did not elicit this effect. Thus, results do not support a role for SP in modulating endometrial function to establish pregnancy in cattle.
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Publication Date: 2019-10-21 PubMed ID: 31636362PubMed Central: PMC6803643DOI: 10.1038/s41598-019-51413-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article is about the impact of bull seminal plasma (SP) and sperm on endometrial function, with a finding that certain SP-RNase can deteriorate the endometrial RNA integrity.

Research Objectives and Methods

  • The main aim of the study was to assess the influence of bull seminal plasma (SP) and sperm on the function of the endometrium. It was postulated that the interaction of the SP with the endometrial tissue may play a role in the establishment of pregnancy.
  • The experiment was conducted using bovine endometrial explants. These explants were exposed to a variety of conditions — ejaculated sperm with or without SP, epididymal sperm, or just SP. The methodology facilitated the observation of specific reactions to these differing scenarios.

Key Findings

  • The study’s results revealed that neither the ejaculated nor the epididymal sperm led to differential expression of a series of inflammatory markers and proteins, which included IL1A, IL1B, IL6, IL8, PTGES2, TNFA, and LIF. This points to the lack of effect of these sperm types on the endometrial function.
  • However, it was found that the SP had a damaging effect on the integrity of the endometrial RNA, suggesting a potentially harmful interaction. This disruptive effect could be blocked by the addition of an RNase inactivation reagent to SP, illustrating a role for SP-RNase in this RNA degradation process.
  • The researchers then theorized that because bulls deposit their ejaculate in the vagina, the bovine endometrium may be more sensitive to SP-RNase than are the vaginal and cervical tissues. This was possibly due to these tissues’ direct exposure to SP during mating.
  • The study also explored the variable effects due to SP-RNase abundance, which was dependent on the method of SP collection—either an artificial vagina (AV) or by electroejaculation (EE). It concluded that bull SP collected by AV does deteriorate the RNA of the mare endometrium and bovine vagina, cervix, and endometrium; however, stallion SP or bull SP collected by EE did not cause this effect.

Conclusion

  • In conclusion, the results of the study do not support the hypothesis that SP plays a role in modulating endometrial function to establish pregnancy in cattle, as it primarily demonstrated a detrimental effect on endometrial RNA integrity. This insight can contribute to our understanding of fertility in cattle and potentially aid in developing more effective breeding practices.

Cite This Article

APA
Fernandez-Fuertes B, Sánchez JM, Bagés-Arnal S, McDonald M, Yeste M, Lonergan P. (2019). Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation. Sci Rep, 9(1), 15072. https://doi.org/10.1038/s41598-019-51413-4

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 9
Issue: 1
Pages: 15072
PII: 15072

Researcher Affiliations

Fernandez-Fuertes, Beatriz
  • Department of Biology, Faculty of Sciences, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain. beatriz.fernandez@udg.edu.
Sánchez, José María
  • School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Bagés-Arnal, Sandra
  • School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
McDonald, Michael
  • School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Yeste, Marc
  • Department of Biology, Faculty of Sciences, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.
Lonergan, Pat
  • School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.

MeSH Terms

  • Animals
  • Cattle
  • Endometrium / metabolism
  • Female
  • Gene Expression Regulation
  • Horses
  • Male
  • RNA Stability
  • Ribonucleases / metabolism
  • Semen / metabolism
  • Species Specificity
  • Specimen Handling / methods

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 4 times.
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