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Archives of virology1996; 141(1); 1-12; doi: 10.1007/BF01718584

Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains.

Abstract: We have sequenced gene 5 encoding NSP1 for three human, two porcine, two bovine, one feline, and five equine rotavirus strains, and compared the nucleotide and deduced amino acid sequences with the published sequences for other various strains. Subgroup I human strains L26, 69M, and DS-1 were found to have a similar NSP1 sequence despite their different G serotypes, VP4 genotypes, and RNA patterns. The NSP1 sequence of the human strain K8 showed a high degree of homology to those of porcine strains OSU and YM. A high degree of homology was found among three equine strains (H2, FI-14, and FI23), but they differed from the other equine strains L338 and H1. The strain H1 was similar to the porcine strains. The feline strain Cat2 showed a high homology to bovine strains UK, RF, and A44. Thus, species-specific and interspecies relatedness of NSP1 sequences among human, porcine, bovine, feline and equine rotaviruses was found. Overall genomic relatedness of strains L26 and YM to various human and animal strains was also examined by RNA-RNA hybridization assay. The present and previous hybridization results showed that there is a good correlation in most strains between overall genomic property (or genogroup) and NSP1 sequence homology.
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Publication Date: 1996-01-01 PubMed ID: 8629937DOI: 10.1007/BF01718584Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper looks into the NSP1 gene sequences in various rotavirus strains found in humans, pigs, cows, cats, and horses. It discusses the shared similarities and differences between these strains, discovering a certain degree of species-specific and interspecies relatedness.

Study Methodology

  • The researchers sequenced the gene 5, which encodes NSP1, for several rotavirus strains found in humans, pigs, cows, cats, and horses.
  • They compared the nucleotide and amino acid sequences with published sequences of various other strains.
  • Additionally, an RNA-RNA hybridization assay was conducted to examine the overall genomic relatedness of select strains (L26 and YM) to various human and animal strains.

Findings of the Study

  • The analysis revealed that Subgroup I human strains L26, 69M, and DS-1 had similar NSP1 sequences, regardless of their differing G serotypes, VP4 genotypes, and RNA patterns.
  • The NSP1 sequence of human strain K8 showed high similarity to the porcine (pig) strains OSU and YM.
  • Among equine (horse) strains H2, FI-14, and FI23, a high degree of similarity was found, but they were different from other equine strains L338 and H1. In fact, strain H1 was more similar to the porcine strains.
  • The feline (cat) strain Cat2 showed significant similarity to bovine (cow) strains UK, RF, and A44.

Implications of the Findings

  • The study confirmed the existence of species-specific and interspecies relatedness in NSP1 sequences in rotaviruses across various species.
  • The RNA-RNA hybridization assay results reinforced that there’s a good correlation in most strains between overall genomic properties (or genogroup) and NSP1 sequence similarity.
  • These findings broaden our understanding of the interspecies transmission and genetic diversification of rotaviruses, which could enhance strategies for the development of vaccines and antiviral therapies.

Cite This Article

APA
Kojima K, Taniguchi K, Kobayashi N. (1996). Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains. Arch Virol, 141(1), 1-12. https://doi.org/10.1007/BF01718584

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 141
Issue: 1
Pages: 1-12

Researcher Affiliations

Kojima, K
  • Department of Hygiene, Sapporo Medical University School of Medicine, Japan.
Taniguchi, K
    Kobayashi, N

      MeSH Terms

      • Amino Acid Sequence
      • Animals
      • Base Sequence
      • Cats
      • Cattle
      • DNA Primers
      • Genes, Viral
      • Horses
      • Humans
      • Molecular Sequence Data
      • Phylogeny
      • Polymerase Chain Reaction
      • RNA, Double-Stranded / metabolism
      • RNA, Messenger / metabolism
      • Rotavirus / classification
      • Rotavirus / genetics
      • Rotavirus / isolation & purification
      • Sequence Homology, Amino Acid
      • Species Specificity
      • Swine
      • Viral Nonstructural Proteins / chemistry
      • Viral Nonstructural Proteins / genetics

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      Citations

      This article has been cited 19 times.
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