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Home / Equine Research Bank / Infect Immun / Species specificity of plasminogen activation and acquisitio...
Infection and immunity1999; 67(12); 6487-6495; doi: 10.1128/IAI.67.12.6487-6495.1999

Species specificity of plasminogen activation and acquisition of surface-associated proteolytic activity by group C streptococci grown in plasma.

Abstract: Our laboratory previously demonstrated that group C streptococcal isolates from humans and horses secrete streptokinases that preferentially activate plasminogens reflecting the origin of the isolates. To analyze the significance of these findings, series of streptokinase-producing Streptococcus equisimilis isolates recovered from humans and horses were examined. Southern blot analysis revealed that chromosomal DNA of the streptococcal isolates from humans reacted exclusively with a skc(hu) probe and that chromosomal DNA of streptococcal isolates from horses reacted preferentially with an skc(eq) probe in a distinct pattern. The streptococcal isolates were examined for the ability to acquire surface-bound plasmin-like activity when grown in the presence of human or equine plasma. Each of eight isolates from humans acquired significant enzymatic activity only when grown in the presence of human plasma, while each of eight isolates from horses acquired activity only when grown in the presence of equine plasma. Analysis of bacterial and host protein requirements indicated critical roles for streptokinase, activatable plasminogen, and fibrinogen. These requirements may explain why certain streptococcal isolates cause disease only in a limited number of mammalian hosts.
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Publication Date: 1999-11-24 PubMed ID: 10569767PubMed Central: PMC97059DOI: 10.1128/IAI.67.12.6487-6495.1999Google Scholar: Lookup
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  • Non-U.S. Gov't
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how different strains of group C streptococci bacteria, stemming from humans and horses, produce particular streptokinases that specifically activate plasminogens corresponding to their origin. The study uncovers substantial roles of streptokinase, fibrinogen, and activatable plasminogen in the bacteria’s ability to cause disease, leading to potential understanding of its host specificity.

Methodology and Findings

  • Initially, streptokinase-producing Streptococcus equisimilis isolates from humans and horses were analysed to understand the implications of earlier findings.
  • Southern blot analysis, a method for detecting specific DNA sequences in DNA samples, was performed and it indicated that human and horse streptococcal isolates reacted exclusively with their corresponding probes, skc(hu) and skc(eq) respectively, illustrating a unique pattern.
  • The study then proceeded to assess the ability of these bacterial isolates to acquire surface-bound plasmin-like activity, a crucial proteolytic function, when grown in human or equine plasma.
  • The results demonstrated that human isolates obtained significant enzymatic activity only when introduced to human plasma. Similarly, the horse isolates reacted significantly only with equine plasma.

Significance of Bacterial and Host Protein Requirements

  • The paper concludes by highlighting the crucial roles of streptokinase, activatable plasminogen, and fibrinogen in these processes.
  • Streptokinase, a bacterial enzyme, interacts with plasminogen, a host protein, to form a complex that activates other plasminogen molecules, leading to the degradation of fibrin, a protein formed during blood clotting.
  • The host-specific activity of streptokinases produced by different bacterial isolates suggest their potential role in determining the host range, i.e., why certain bacterial isolates cause disease only in specific hosts.

Conclusions and Implications

  • These findings offer insight into the mechanism behind host-specificity of bacterial infections – more specifically, how interactions between bacterial and host proteins could influence the host range of infection-causing bacteria.
  • Understanding these interactions may provide crucial knowledge about the pathogenesis of streptococcal infections and can help design better treatment strategies.
  • The study also reveals the potential of southern blot analysis as an effective tool in studying host-pathogen interaction and infection biology.

Cite This Article

APA
Schroeder B, Boyle MD, Sheerin BR, Asbury AC, Lottenberg R. (1999). Species specificity of plasminogen activation and acquisition of surface-associated proteolytic activity by group C streptococci grown in plasma. Infect Immun, 67(12), 6487-6495. https://doi.org/10.1128/IAI.67.12.6487-6495.1999

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 67
Issue: 12
Pages: 6487-6495

Researcher Affiliations

Schroeder, B
  • Division of Hematology and Oncology, Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA.
Boyle, M D
    Sheerin, B R
      Asbury, A C
        Lottenberg, R

          MeSH Terms

          • Animals
          • Blood
          • Culture Media
          • Fibrinogen / metabolism
          • Horse Diseases / microbiology
          • Horses
          • Humans
          • Nucleic Acid Hybridization
          • Plasminogen / metabolism
          • Plasminogen Activators / metabolism
          • Species Specificity
          • Streptococcal Infections / microbiology
          • Streptococcal Infections / veterinary
          • Streptococcus / classification
          • Streptococcus / enzymology
          • Streptococcus / genetics
          • Streptococcus / growth & development
          • Streptococcus / isolation & purification
          • Streptokinase / genetics
          • Streptokinase / metabolism

          Grant Funding

          • AI43474 / NIAID NIH HHS
          • HL41898 / NHLBI NIH HHS

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          This article has been cited 9 times.
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